Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production

Biological pretreatment of lignocellulosic biomass is considered to be energy-efficient and cost-effective. In the present study, sorghum husk was biologically pretreated with a white-rot fungus <em>Phanerochaete chrysosporium </em>(MTCC 4955) under submerged static condition. Ligninolyt...

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Main Authors: Pankajkumar R. Waghmare, Rahul V. Khandare, Byong-Hun Jeon, Sanjay P. Govindwar
Format: Article
Language:English
Published: Alpha Creation Enterprise 2018-09-01
Series:Biofuel Research Journal
Subjects:
Online Access:http://www.biofueljournal.com/article_68378_92132cce3e76540bc9460dfcf384ee9b.pdf
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author Pankajkumar R. Waghmare
Rahul V. Khandare
Byong-Hun Jeon
Sanjay P. Govindwar
author_facet Pankajkumar R. Waghmare
Rahul V. Khandare
Byong-Hun Jeon
Sanjay P. Govindwar
author_sort Pankajkumar R. Waghmare
collection DOAJ
description Biological pretreatment of lignocellulosic biomass is considered to be energy-efficient and cost-effective. In the present study, sorghum husk was biologically pretreated with a white-rot fungus <em>Phanerochaete chrysosporium </em>(MTCC 4955) under submerged static condition. Ligninolytic enzymes like lignin peroxidase (0.843 U/mL) and manganese peroxidase (0.389 U/mL) played an important role in the biological pretreatment of sorghum husk. Activities of different hydrolytic enzymes such as endoglucanase (57.25 U/mL), exoglucanase (4.76 U/mL), filter paperase (0.580 U/mL), glucoamylase (153.38 U/mL), and xylanase (88.14 U/mL) during biological pretreatment of sorghum husk by <em>P. chrysosporium </em>were evaluated. Enzymatic hydrolysis of untreated sorghum husk and biologically pretreated sorghum husk produced 20.07 and 103.0 mg/g reducing sugars, respectively. This result showed a significant increase in reducing sugar production in the biologically pretreated sorghum husk as compared to its untreated counterpart. Biologically pretreated sorghum husk hydrolysate was further fermented for 48 h using <em>Saccharomyces cerevisiae</em> (KCTC 7296), <em>Pachysolen tannophilus</em> (MTCC 1077), and their co-culture resulting in ethanol yields of 2.113, 1.095, and 2.348%, respectively. The surface characteristics of the substrate were evaluated after the delignification and hydrolysis, using FTIR, XRD, and SEM, confirming the effectiveness of the biological pretreatment process.
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spelling doaj.art-7077885f5989405e98ca37ede20e1f672024-03-26T15:13:22ZengAlpha Creation EnterpriseBiofuel Research Journal2292-87822292-87822018-09-015384685310.18331/BRJ2018.5.3.468378Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol productionPankajkumar R. Waghmare0Rahul V. Khandare1Byong-Hun Jeon2Sanjay P. Govindwar3Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur 416004, Maharashtra, India.|State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China.Amity Institute of Biotechnology, Amity University, Mumbai, India 410206.Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul 04763, South Korea.Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur 416004, Maharashtra, India.|Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul 04763, South Korea.Biological pretreatment of lignocellulosic biomass is considered to be energy-efficient and cost-effective. In the present study, sorghum husk was biologically pretreated with a white-rot fungus <em>Phanerochaete chrysosporium </em>(MTCC 4955) under submerged static condition. Ligninolytic enzymes like lignin peroxidase (0.843 U/mL) and manganese peroxidase (0.389 U/mL) played an important role in the biological pretreatment of sorghum husk. Activities of different hydrolytic enzymes such as endoglucanase (57.25 U/mL), exoglucanase (4.76 U/mL), filter paperase (0.580 U/mL), glucoamylase (153.38 U/mL), and xylanase (88.14 U/mL) during biological pretreatment of sorghum husk by <em>P. chrysosporium </em>were evaluated. Enzymatic hydrolysis of untreated sorghum husk and biologically pretreated sorghum husk produced 20.07 and 103.0 mg/g reducing sugars, respectively. This result showed a significant increase in reducing sugar production in the biologically pretreated sorghum husk as compared to its untreated counterpart. Biologically pretreated sorghum husk hydrolysate was further fermented for 48 h using <em>Saccharomyces cerevisiae</em> (KCTC 7296), <em>Pachysolen tannophilus</em> (MTCC 1077), and their co-culture resulting in ethanol yields of 2.113, 1.095, and 2.348%, respectively. The surface characteristics of the substrate were evaluated after the delignification and hydrolysis, using FTIR, XRD, and SEM, confirming the effectiveness of the biological pretreatment process.http://www.biofueljournal.com/article_68378_92132cce3e76540bc9460dfcf384ee9b.pdfSorghum huskPhanerochaete chrysosporiumDelignificationBiological pretreatmentEnzymatic hydrolysisEthanol production
spellingShingle Pankajkumar R. Waghmare
Rahul V. Khandare
Byong-Hun Jeon
Sanjay P. Govindwar
Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production
Biofuel Research Journal
Sorghum husk
Phanerochaete chrysosporium
Delignification
Biological pretreatment
Enzymatic hydrolysis
Ethanol production
title Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production
title_full Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production
title_fullStr Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production
title_full_unstemmed Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production
title_short Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production
title_sort enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production
topic Sorghum husk
Phanerochaete chrysosporium
Delignification
Biological pretreatment
Enzymatic hydrolysis
Ethanol production
url http://www.biofueljournal.com/article_68378_92132cce3e76540bc9460dfcf384ee9b.pdf
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AT byonghunjeon enzymatichydrolysisofbiologicallypretreatedsorghumhuskforbioethanolproduction
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