Enhanced Polyphenols Recovery from Grape Pomace: A Comparison of Pressurized and Atmospheric Extractions with Deep Eutectic Solvent Aqueous Mixtures
Deep eutectic solvents (DES) are emerging as potent polyphenol extractors under normal atmospheric conditions. Yet, their effectiveness in hot pressurized liquid extraction (HPLE) must be studied more. We explored the ability of various water/DES and water/hydrogen bond donors (HBDs) mixtures in bot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/7/1446 |
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| author | Nils Leander Huamán-Castilla Nicolás Gajardo-Parra José R. Pérez-Correa Roberto I. Canales Maximiliano Martínez-Cifuentes Gabriela Contreras-Contreras María Salomé Mariotti-Celis |
| author_facet | Nils Leander Huamán-Castilla Nicolás Gajardo-Parra José R. Pérez-Correa Roberto I. Canales Maximiliano Martínez-Cifuentes Gabriela Contreras-Contreras María Salomé Mariotti-Celis |
| author_sort | Nils Leander Huamán-Castilla |
| collection | DOAJ |
| description | Deep eutectic solvents (DES) are emerging as potent polyphenol extractors under normal atmospheric conditions. Yet, their effectiveness in hot pressurized liquid extraction (HPLE) must be studied more. We explored the ability of various water/DES and water/hydrogen bond donors (HBDs) mixtures in both atmospheric solid liquid extraction (ASLE) and HPLE (50%, 90 °C) for isolating specific polyphenol families from Carménère grape pomace. We assessed extraction yields based on total polyphenols, antioxidant capacity, and recovery of targeted polyphenols. The HBDs ethylene glycol and glycerol outperformed DES in atmospheric and pressurized extractions. Ethylene glycol exhibited a higher affinity for phenolic acids and flavonols, while flavanols preferred glycerol. Quantum chemical computations indicated that a high-water content in DES mixtures led to the formation of new hydrogen bonds, thereby reducing polyphenol-solvent interactions. HPLE was found to be superior to ASLE across all tested solvents. The elevated pressure in HPLE has caused significant improvement in the recovery of flavanols (17–89%), phenolic acids (17–1000%), and flavonols (81–258%). Scanning electron microscopy analysis of post-extraction residues suggested that high pressures collapse the plant matrix, thus easing polyphenol release. |
| first_indexed | 2024-03-11T01:21:38Z |
| format | Article |
| id | doaj.art-70784b9adbbe4adf833a701897af3e85 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T01:21:38Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-70784b9adbbe4adf833a701897af3e852023-11-18T18:06:05ZengMDPI AGAntioxidants2076-39212023-07-01127144610.3390/antiox12071446Enhanced Polyphenols Recovery from Grape Pomace: A Comparison of Pressurized and Atmospheric Extractions with Deep Eutectic Solvent Aqueous MixturesNils Leander Huamán-Castilla0Nicolás Gajardo-Parra1José R. Pérez-Correa2Roberto I. Canales3Maximiliano Martínez-Cifuentes4Gabriela Contreras-Contreras5María Salomé Mariotti-Celis6Escuela de Ingeniería Agroindustrial, Universidad Nacional de Moquegua, Prolongación Calle Ancash s/n, Moquegua 18001, PeruChemical and Bioprocess Engineering Department, School of Engineering, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, P.O. Box 306, Santiago 7820436, ChileChemical and Bioprocess Engineering Department, School of Engineering, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, P.O. Box 306, Santiago 7820436, ChileChemical and Bioprocess Engineering Department, School of Engineering, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, P.O. Box 306, Santiago 7820436, ChileDepartamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Edmundo Larenas 129, Concepción 4070371, ChileEscuela de Nutrición y Dietética, Facultad de Medicina, Universidad Finis Terrae, Santiago 7501015, ChileEscuela de Nutrición y Dietética, Facultad de Medicina, Universidad Finis Terrae, Santiago 7501015, ChileDeep eutectic solvents (DES) are emerging as potent polyphenol extractors under normal atmospheric conditions. Yet, their effectiveness in hot pressurized liquid extraction (HPLE) must be studied more. We explored the ability of various water/DES and water/hydrogen bond donors (HBDs) mixtures in both atmospheric solid liquid extraction (ASLE) and HPLE (50%, 90 °C) for isolating specific polyphenol families from Carménère grape pomace. We assessed extraction yields based on total polyphenols, antioxidant capacity, and recovery of targeted polyphenols. The HBDs ethylene glycol and glycerol outperformed DES in atmospheric and pressurized extractions. Ethylene glycol exhibited a higher affinity for phenolic acids and flavonols, while flavanols preferred glycerol. Quantum chemical computations indicated that a high-water content in DES mixtures led to the formation of new hydrogen bonds, thereby reducing polyphenol-solvent interactions. HPLE was found to be superior to ASLE across all tested solvents. The elevated pressure in HPLE has caused significant improvement in the recovery of flavanols (17–89%), phenolic acids (17–1000%), and flavonols (81–258%). Scanning electron microscopy analysis of post-extraction residues suggested that high pressures collapse the plant matrix, thus easing polyphenol release.https://www.mdpi.com/2076-3921/12/7/1446grape pomacepolyphenolsantioxidant capacitydeep eutectic solventshot pressurized liquid extractionatmospheric extraction |
| spellingShingle | Nils Leander Huamán-Castilla Nicolás Gajardo-Parra José R. Pérez-Correa Roberto I. Canales Maximiliano Martínez-Cifuentes Gabriela Contreras-Contreras María Salomé Mariotti-Celis Enhanced Polyphenols Recovery from Grape Pomace: A Comparison of Pressurized and Atmospheric Extractions with Deep Eutectic Solvent Aqueous Mixtures Antioxidants grape pomace polyphenols antioxidant capacity deep eutectic solvents hot pressurized liquid extraction atmospheric extraction |
| title | Enhanced Polyphenols Recovery from Grape Pomace: A Comparison of Pressurized and Atmospheric Extractions with Deep Eutectic Solvent Aqueous Mixtures |
| title_full | Enhanced Polyphenols Recovery from Grape Pomace: A Comparison of Pressurized and Atmospheric Extractions with Deep Eutectic Solvent Aqueous Mixtures |
| title_fullStr | Enhanced Polyphenols Recovery from Grape Pomace: A Comparison of Pressurized and Atmospheric Extractions with Deep Eutectic Solvent Aqueous Mixtures |
| title_full_unstemmed | Enhanced Polyphenols Recovery from Grape Pomace: A Comparison of Pressurized and Atmospheric Extractions with Deep Eutectic Solvent Aqueous Mixtures |
| title_short | Enhanced Polyphenols Recovery from Grape Pomace: A Comparison of Pressurized and Atmospheric Extractions with Deep Eutectic Solvent Aqueous Mixtures |
| title_sort | enhanced polyphenols recovery from grape pomace a comparison of pressurized and atmospheric extractions with deep eutectic solvent aqueous mixtures |
| topic | grape pomace polyphenols antioxidant capacity deep eutectic solvents hot pressurized liquid extraction atmospheric extraction |
| url | https://www.mdpi.com/2076-3921/12/7/1446 |
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