A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors
Abstract An insulator-based dielectrophoresis (iDEP) is a label-free method that has been extensively utilized for manipulation of nanoparticles, cells, and biomolecules. Here, we present a new iDEP approach that can rapidly trap nanoparticles at the close proximity of a glass nanopipette’s tip by a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-25026-2 |
| _version_ | 1818843403426725888 |
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| author | Leilei Shi Ankit Rana Leyla Esfandiari |
| author_facet | Leilei Shi Ankit Rana Leyla Esfandiari |
| author_sort | Leilei Shi |
| collection | DOAJ |
| description | Abstract An insulator-based dielectrophoresis (iDEP) is a label-free method that has been extensively utilized for manipulation of nanoparticles, cells, and biomolecules. Here, we present a new iDEP approach that can rapidly trap nanoparticles at the close proximity of a glass nanopipette’s tip by applying 10 V/cm direct current (DC) across the pipette’s length. The trapping mechanism was systemically studied using both numerical modeling and experimental observations. The results showed that the particle trapping was determined to be controlled by three dominant electrokinetic forces including dielectrophoretic, electrophoretic and electroosmotic force. Furthermore, the effect of the ionic strength, the pipette’s geometry, and the applied electric field on the entrapment efficiency was investigated. To show the application of our device in biomedical sciences, we demonstrated the successful entrapment of fluorescently tagged liposomes and unlabeled plasma-driven exosomes from the PBS solution. Also, to illustrate the selective entrapment capability of our device, 100 nm liposomes were extracted from the PBS solution containing 500 nm polystyrene particles at the tip of the pipette as the voltage polarity was reversed. |
| first_indexed | 2024-12-19T04:57:19Z |
| format | Article |
| id | doaj.art-7079644c2a114d5dacd1585e84e37da4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:57:19Z |
| publishDate | 2018-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7079644c2a114d5dacd1585e84e37da42022-12-21T20:35:12ZengNature PortfolioScientific Reports2045-23222018-04-018111210.1038/s41598-018-25026-2A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donorsLeilei Shi0Ankit Rana1Leyla Esfandiari2Department of Electrical Engineering and Computer Science, College of Engineering and Applied Science, University of CincinnatiDepartment of Electrical Engineering and Computer Science, College of Engineering and Applied Science, University of CincinnatiDepartment of Electrical Engineering and Computer Science, College of Engineering and Applied Science, University of CincinnatiAbstract An insulator-based dielectrophoresis (iDEP) is a label-free method that has been extensively utilized for manipulation of nanoparticles, cells, and biomolecules. Here, we present a new iDEP approach that can rapidly trap nanoparticles at the close proximity of a glass nanopipette’s tip by applying 10 V/cm direct current (DC) across the pipette’s length. The trapping mechanism was systemically studied using both numerical modeling and experimental observations. The results showed that the particle trapping was determined to be controlled by three dominant electrokinetic forces including dielectrophoretic, electrophoretic and electroosmotic force. Furthermore, the effect of the ionic strength, the pipette’s geometry, and the applied electric field on the entrapment efficiency was investigated. To show the application of our device in biomedical sciences, we demonstrated the successful entrapment of fluorescently tagged liposomes and unlabeled plasma-driven exosomes from the PBS solution. Also, to illustrate the selective entrapment capability of our device, 100 nm liposomes were extracted from the PBS solution containing 500 nm polystyrene particles at the tip of the pipette as the voltage polarity was reversed.https://doi.org/10.1038/s41598-018-25026-2 |
| spellingShingle | Leilei Shi Ankit Rana Leyla Esfandiari A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors Scientific Reports |
| title | A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors |
| title_full | A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors |
| title_fullStr | A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors |
| title_full_unstemmed | A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors |
| title_short | A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors |
| title_sort | low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors |
| url | https://doi.org/10.1038/s41598-018-25026-2 |
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