Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning
Vertebrate embryonic patterning depends on signaling from Nodal, a TGFβ superfamily member. There are three Nodal orthologs in zebrafish; southpaw directs left-right asymmetries, while squint and cyclops function earlier to pattern mesendoderm. TGFβ member Vg1 is implicated in mesoderm formation but...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/28635 |
| _version_ | 1811200242207424512 |
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| author | Jose L Pelliccia Granton A Jindal Rebecca D Burdine |
| author_facet | Jose L Pelliccia Granton A Jindal Rebecca D Burdine |
| author_sort | Jose L Pelliccia |
| collection | DOAJ |
| description | Vertebrate embryonic patterning depends on signaling from Nodal, a TGFβ superfamily member. There are three Nodal orthologs in zebrafish; southpaw directs left-right asymmetries, while squint and cyclops function earlier to pattern mesendoderm. TGFβ member Vg1 is implicated in mesoderm formation but the role of the zebrafish ortholog, Growth differentiation factor 3 (Gdf3), has not been fully explored. We show that zygotic expression of gdf3 is dispensable for embryonic development, while maternally deposited gdf3 is required for mesendoderm formation and dorsal-ventral patterning. We further show that Gdf3 can affect left-right patterning at multiple stages, including proper development of regional cell morphology in Kupffer’s vesicle and the establishment of southpaw expression in the lateral plate mesoderm. Collectively, our data indicate that gdf3 is critical for robust Nodal signaling at multiple stages in zebrafish embryonic development. |
| first_indexed | 2024-04-12T02:01:17Z |
| format | Article |
| id | doaj.art-7079c9c1e46c40409ec16d150efa8f6c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:01:17Z |
| publishDate | 2017-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7079c9c1e46c40409ec16d150efa8f6c2022-12-22T03:52:40ZengeLife Sciences Publications LtdeLife2050-084X2017-11-01610.7554/eLife.28635Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterningJose L Pelliccia0https://orcid.org/0000-0002-8452-2126Granton A Jindal1https://orcid.org/0000-0003-2803-9831Rebecca D Burdine2https://orcid.org/0000-0001-6620-5015Department of Molecular Biology, Princeton University, Princeton, United StatesDepartment of Molecular Biology, Princeton University, Princeton, United States; Department of Chemical and Biological Engineering, Princeton University, Princeton, United States; The Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, United StatesDepartment of Molecular Biology, Princeton University, Princeton, United StatesVertebrate embryonic patterning depends on signaling from Nodal, a TGFβ superfamily member. There are three Nodal orthologs in zebrafish; southpaw directs left-right asymmetries, while squint and cyclops function earlier to pattern mesendoderm. TGFβ member Vg1 is implicated in mesoderm formation but the role of the zebrafish ortholog, Growth differentiation factor 3 (Gdf3), has not been fully explored. We show that zygotic expression of gdf3 is dispensable for embryonic development, while maternally deposited gdf3 is required for mesendoderm formation and dorsal-ventral patterning. We further show that Gdf3 can affect left-right patterning at multiple stages, including proper development of regional cell morphology in Kupffer’s vesicle and the establishment of southpaw expression in the lateral plate mesoderm. Collectively, our data indicate that gdf3 is critical for robust Nodal signaling at multiple stages in zebrafish embryonic development.https://elifesciences.org/articles/28635Gdf3Vg1Nodalleft-right patterningKupffer's vesiclemesendoderm |
| spellingShingle | Jose L Pelliccia Granton A Jindal Rebecca D Burdine Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning eLife Gdf3 Vg1 Nodal left-right patterning Kupffer's vesicle mesendoderm |
| title | Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning |
| title_full | Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning |
| title_fullStr | Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning |
| title_full_unstemmed | Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning |
| title_short | Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning |
| title_sort | gdf3 is required for robust nodal signaling during germ layer formation and left right patterning |
| topic | Gdf3 Vg1 Nodal left-right patterning Kupffer's vesicle mesendoderm |
| url | https://elifesciences.org/articles/28635 |
| work_keys_str_mv | AT joselpelliccia gdf3isrequiredforrobustnodalsignalingduringgermlayerformationandleftrightpatterning AT grantonajindal gdf3isrequiredforrobustnodalsignalingduringgermlayerformationandleftrightpatterning AT rebeccadburdine gdf3isrequiredforrobustnodalsignalingduringgermlayerformationandleftrightpatterning |