Inhibitory effects and oxidative damages in Cladophora sp. (Cladophoraceae) exposed to berberine
Cladophora members are present in seawater and freshwater ecosystems worldwide. Their growth poses a serious threat to water environment, fisheries, production and living. In order to explore safe and ecological treatment methods, the inhibitory effects and oxidative damages (48 and 96 h) in Cladoph...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422003532 |
| _version_ | 1811185923800432640 |
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| author | Yongtao Tang Luping Wang Liangjie Zhao Chen Qian Feng Lun Chen Wang Hui Zheng Boping Tang Yongxu Cheng Xusheng Guo |
| author_facet | Yongtao Tang Luping Wang Liangjie Zhao Chen Qian Feng Lun Chen Wang Hui Zheng Boping Tang Yongxu Cheng Xusheng Guo |
| author_sort | Yongtao Tang |
| collection | DOAJ |
| description | Cladophora members are present in seawater and freshwater ecosystems worldwide. Their growth poses a serious threat to water environment, fisheries, production and living. In order to explore safe and ecological treatment methods, the inhibitory effects and oxidative damages (48 and 96 h) in Cladophora sp. (Cladophoraceae) exposed to 0–0.30 g L−1 berberine were investigated. Results showed that the LC 50 of berberine to Cladophora was 0.147 g L−1 and 0.063 g L−1 at 48 h and 96 h, respectively. Malondialdehyde and total protein contents first increased and then decreased sharply with increasing exposure concentrations of berberine. The Cladophora cells accelerated protein synthesis when their cell membranes were subjected to oxidative damage. Superoxide dismutase activity was down-regulated slightly after exposure to low berberine concentration (0.05 g L−1) and strongly when the Cladophora cells suffered from great oxidative damage. Total antioxidant capacity (T-AOC) first decreased sharply and then increased with increasing exposure concentrations of berberine. The decrease in T-AOC indicated the enzymatic antioxidants were continuously inhibited with increasing exposure concentrations of berberine. The increase in T-AOC indicated the secretion of non-enzymatic antioxidants was continuously strengthened with increasing exposure concentrations of berberine. Cladophora DNA also underwent oxidative damage due to berberine. Low concentrations of berberine activated its repair mechanisms, but high concentrations could cause irreparable damage. Therefore, these results suggested that berberine could inhibit the growth of Cladophora by oxidative damage, and Cladophora responded physiologically to this stress accordingly. |
| first_indexed | 2024-04-11T13:38:46Z |
| format | Article |
| id | doaj.art-707a02b62f614432b3d8d086a5caed27 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-11T13:38:46Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-707a02b62f614432b3d8d086a5caed272022-12-22T04:21:23ZengElsevierAquaculture Reports2352-51342022-12-0127101357Inhibitory effects and oxidative damages in Cladophora sp. (Cladophoraceae) exposed to berberineYongtao Tang0Luping Wang1Liangjie Zhao2Chen Qian3Feng Lun4Chen Wang5Hui Zheng6Boping Tang7Yongxu Cheng8Xusheng Guo9College of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China; Key Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, ChinaKey Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, ChinaKey Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKoruk Lake Fisheries Enterprise, Delingha 817000, Qinghai, ChinaYancheng Teachers University, Yancheng 224300, Jiangsu, ChinaKey Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Correspondence to: College of Fisheries and Life Science, Shanghai Ocean University, 999# Huchenghuan Road, Pudong District, Shanghai 201306, PR China.College of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China; Correspondence to: College of Fisheries, Xinyang Agriculture and Forestry University, No.1# Beihuan Road, Xinyang city, Henan 464000, PR China.Cladophora members are present in seawater and freshwater ecosystems worldwide. Their growth poses a serious threat to water environment, fisheries, production and living. In order to explore safe and ecological treatment methods, the inhibitory effects and oxidative damages (48 and 96 h) in Cladophora sp. (Cladophoraceae) exposed to 0–0.30 g L−1 berberine were investigated. Results showed that the LC 50 of berberine to Cladophora was 0.147 g L−1 and 0.063 g L−1 at 48 h and 96 h, respectively. Malondialdehyde and total protein contents first increased and then decreased sharply with increasing exposure concentrations of berberine. The Cladophora cells accelerated protein synthesis when their cell membranes were subjected to oxidative damage. Superoxide dismutase activity was down-regulated slightly after exposure to low berberine concentration (0.05 g L−1) and strongly when the Cladophora cells suffered from great oxidative damage. Total antioxidant capacity (T-AOC) first decreased sharply and then increased with increasing exposure concentrations of berberine. The decrease in T-AOC indicated the enzymatic antioxidants were continuously inhibited with increasing exposure concentrations of berberine. The increase in T-AOC indicated the secretion of non-enzymatic antioxidants was continuously strengthened with increasing exposure concentrations of berberine. Cladophora DNA also underwent oxidative damage due to berberine. Low concentrations of berberine activated its repair mechanisms, but high concentrations could cause irreparable damage. Therefore, these results suggested that berberine could inhibit the growth of Cladophora by oxidative damage, and Cladophora responded physiologically to this stress accordingly.http://www.sciencedirect.com/science/article/pii/S2352513422003532BerberineCladophoraInhibitory effectsOxidative damageStress |
| spellingShingle | Yongtao Tang Luping Wang Liangjie Zhao Chen Qian Feng Lun Chen Wang Hui Zheng Boping Tang Yongxu Cheng Xusheng Guo Inhibitory effects and oxidative damages in Cladophora sp. (Cladophoraceae) exposed to berberine Aquaculture Reports Berberine Cladophora Inhibitory effects Oxidative damage Stress |
| title | Inhibitory effects and oxidative damages in Cladophora sp. (Cladophoraceae) exposed to berberine |
| title_full | Inhibitory effects and oxidative damages in Cladophora sp. (Cladophoraceae) exposed to berberine |
| title_fullStr | Inhibitory effects and oxidative damages in Cladophora sp. (Cladophoraceae) exposed to berberine |
| title_full_unstemmed | Inhibitory effects and oxidative damages in Cladophora sp. (Cladophoraceae) exposed to berberine |
| title_short | Inhibitory effects and oxidative damages in Cladophora sp. (Cladophoraceae) exposed to berberine |
| title_sort | inhibitory effects and oxidative damages in cladophora sp cladophoraceae exposed to berberine |
| topic | Berberine Cladophora Inhibitory effects Oxidative damage Stress |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422003532 |
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