Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats
<i>Background and Objectives:</i> Diabetic peripheral neuropathy (DPN) affects approximately half of patients with diabetes mellitus (DM), contributing to falls and fractures. Oxidative stress, which is linked to DM-induced hyperglycemia, has been implicated in the onset of DPN. Although...
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MDPI AG
2024-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/60/3/480 |
| _version_ | 1797240070114115584 |
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| author | Koji Nonaka Junichi Akiyama Satsuki Une |
| author_facet | Koji Nonaka Junichi Akiyama Satsuki Une |
| author_sort | Koji Nonaka |
| collection | DOAJ |
| description | <i>Background and Objectives:</i> Diabetic peripheral neuropathy (DPN) affects approximately half of patients with diabetes mellitus (DM), contributing to falls and fractures. Oxidative stress, which is linked to DM-induced hyperglycemia, has been implicated in the onset of DPN. Although exercise is recommended for patients with DM, its effect on DPN remains unclear. Therefore, this study aimed to investigate the effect of exercise on DPN and the mechanisms involved. <i>Material and Methods</i>: Thirty male Wistar rats were divided into control, streptozotocin (STZ)-induced diabetic (DM), and STZ-induced diabetic/exercise (DM + Ex) groups. Diabetes was induced using STZ injection. Rats in the DM + Ex groups underwent six weeks of treadmill exercise. Sciatic nerve parameters, which included motor nerve conduction velocity (MNCV), antioxidant enzymes (catalase, glutathione peroxidase [GPx], and superoxide dismutase [SOD]), oxidative stress markers (malondialdehyde [MDA] and 4-hydroxy-2-nonenal [4HNE]), and neurotrophic factors (brain-derived neurotrophic factor [BDNF] and nerve growth factor [NGF]), were examined. <i>Results:</i> Exercise alleviated DM-induced decreases in MNCV in rats. Although exercise did not significantly affect antioxidant enzyme activity, 4HNE levels increased significantly, indicating increased oxidative stress. Additionally, exercise did not significantly affect DM-induced increases in NGF and BDNF levels in rats. <i>Conclusions:</i> Exercise may prevent DPN in rats with DM, possibly through nonantioxidant mechanisms. |
| first_indexed | 2024-04-24T18:01:35Z |
| format | Article |
| id | doaj.art-707c62b129aa49cd9307d6e9f8d564f5 |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-04-24T18:01:35Z |
| publishDate | 2024-03-01 |
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| series | Medicina |
| spelling | doaj.art-707c62b129aa49cd9307d6e9f8d564f52024-03-27T13:53:47ZengMDPI AGMedicina1010-660X1648-91442024-03-0160348010.3390/medicina60030480Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic RatsKoji Nonaka0Junichi Akiyama1Satsuki Une2Faculty of Health Sciences, Naragakuen University, Nara 631-8524, Nara, JapanDepartment of Physical Therapy, School of Health Care and Social Welfare, Kibi International University, Takahashi 716-8505, Okayama, JapanFaculty of Education, Kagawa University, Takamatsu 760-8521, Kagawa, Japan<i>Background and Objectives:</i> Diabetic peripheral neuropathy (DPN) affects approximately half of patients with diabetes mellitus (DM), contributing to falls and fractures. Oxidative stress, which is linked to DM-induced hyperglycemia, has been implicated in the onset of DPN. Although exercise is recommended for patients with DM, its effect on DPN remains unclear. Therefore, this study aimed to investigate the effect of exercise on DPN and the mechanisms involved. <i>Material and Methods</i>: Thirty male Wistar rats were divided into control, streptozotocin (STZ)-induced diabetic (DM), and STZ-induced diabetic/exercise (DM + Ex) groups. Diabetes was induced using STZ injection. Rats in the DM + Ex groups underwent six weeks of treadmill exercise. Sciatic nerve parameters, which included motor nerve conduction velocity (MNCV), antioxidant enzymes (catalase, glutathione peroxidase [GPx], and superoxide dismutase [SOD]), oxidative stress markers (malondialdehyde [MDA] and 4-hydroxy-2-nonenal [4HNE]), and neurotrophic factors (brain-derived neurotrophic factor [BDNF] and nerve growth factor [NGF]), were examined. <i>Results:</i> Exercise alleviated DM-induced decreases in MNCV in rats. Although exercise did not significantly affect antioxidant enzyme activity, 4HNE levels increased significantly, indicating increased oxidative stress. Additionally, exercise did not significantly affect DM-induced increases in NGF and BDNF levels in rats. <i>Conclusions:</i> Exercise may prevent DPN in rats with DM, possibly through nonantioxidant mechanisms.https://www.mdpi.com/1648-9144/60/3/480diabetes mellitusperipheral neuropathyexerciseoxidative stressantioxidant enzymesneurotrophic factors |
| spellingShingle | Koji Nonaka Junichi Akiyama Satsuki Une Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats Medicina diabetes mellitus peripheral neuropathy exercise oxidative stress antioxidant enzymes neurotrophic factors |
| title | Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats |
| title_full | Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats |
| title_fullStr | Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats |
| title_full_unstemmed | Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats |
| title_short | Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats |
| title_sort | exercise may increase oxidative stress in the sciatic nerve in streptozotocin induced diabetic rats |
| topic | diabetes mellitus peripheral neuropathy exercise oxidative stress antioxidant enzymes neurotrophic factors |
| url | https://www.mdpi.com/1648-9144/60/3/480 |
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