Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of <i>Saccharomyces pastorianus</i> from <i>Dekkera</i> spp.
Traceability analysis, such as identification and discrimination of yeasts used for fermentation, is important for ensuring manufacturing efficiency and product safety during brewing. However, conventional methods based on morphological and physiological properties have disadvantages such as time co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/8/272 |
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| author | Kazumichi Yokota Asae Takeo Hiroko Abe Yuji Kurokawa Muneaki Hashimoto Kazuaki Kajimoto Masato Tanaka Sanae Murayama Yoshihiro Nakajima Masateru Taniguchi Masatoshi Kataoka |
| author_facet | Kazumichi Yokota Asae Takeo Hiroko Abe Yuji Kurokawa Muneaki Hashimoto Kazuaki Kajimoto Masato Tanaka Sanae Murayama Yoshihiro Nakajima Masateru Taniguchi Masatoshi Kataoka |
| author_sort | Kazumichi Yokota |
| collection | DOAJ |
| description | Traceability analysis, such as identification and discrimination of yeasts used for fermentation, is important for ensuring manufacturing efficiency and product safety during brewing. However, conventional methods based on morphological and physiological properties have disadvantages such as time consumption and low sensitivity. In this study, the resistive pulse method (RPM) was employed to discriminate between <i>Saccharomyces pastorianus</i> and <i>Dekkera anomala</i> and <i>S. pastorianus</i> and <i>D. bruxellensis</i> by measuring the ionic current response of cells flowing through a microsized pore. The height and shape of the pulse signal were used for the simultaneous measurement of the size, shape, and surface charge of individual cells. Accurate discrimination of <i>S. pastorianus</i> from <i>Dekkera</i> spp. was observed with a recall rate of 96.3 ± 0.8%. Furthermore, budding <i>S. pastorianus</i> was quantitatively detected by evaluating the shape of the waveform of the current ionic blockade. We showed a proof-of-concept demonstration of RPM for the detection of contamination of <i>Dekkera</i> spp. in <i>S. pastorianus</i> and for monitoring the fermentation of <i>S. pastorianus</i> through the quantitative detection of budding cells. |
| first_indexed | 2024-03-10T08:58:28Z |
| format | Article |
| id | doaj.art-707d4957795043adace270ddb07e227c |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T08:58:28Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-707d4957795043adace270ddb07e227c2023-11-22T06:57:40ZengMDPI AGBiosensors2079-63742021-08-0111827210.3390/bios11080272Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of <i>Saccharomyces pastorianus</i> from <i>Dekkera</i> spp.Kazumichi Yokota0Asae Takeo1Hiroko Abe2Yuji Kurokawa3Muneaki Hashimoto4Kazuaki Kajimoto5Masato Tanaka6Sanae Murayama7Yoshihiro Nakajima8Masateru Taniguchi9Masatoshi Kataoka10Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, JapanInstitute for Future Beverages, Research & Development Division, Kirin Holdings Company, Limited. 1-17-1, Namamugi, Tsurumi-ku, Yokohama, Kanagawa 230-8628, JapanHealth and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, JapanInstitute for Future Beverages, Research & Development Division, Kirin Holdings Company, Limited. 1-17-1, Namamugi, Tsurumi-ku, Yokohama, Kanagawa 230-8628, JapanHealth and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, JapanHealth and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, JapanHealth and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, JapanHealth and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, JapanHealth and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, JapanTraceability analysis, such as identification and discrimination of yeasts used for fermentation, is important for ensuring manufacturing efficiency and product safety during brewing. However, conventional methods based on morphological and physiological properties have disadvantages such as time consumption and low sensitivity. In this study, the resistive pulse method (RPM) was employed to discriminate between <i>Saccharomyces pastorianus</i> and <i>Dekkera anomala</i> and <i>S. pastorianus</i> and <i>D. bruxellensis</i> by measuring the ionic current response of cells flowing through a microsized pore. The height and shape of the pulse signal were used for the simultaneous measurement of the size, shape, and surface charge of individual cells. Accurate discrimination of <i>S. pastorianus</i> from <i>Dekkera</i> spp. was observed with a recall rate of 96.3 ± 0.8%. Furthermore, budding <i>S. pastorianus</i> was quantitatively detected by evaluating the shape of the waveform of the current ionic blockade. We showed a proof-of-concept demonstration of RPM for the detection of contamination of <i>Dekkera</i> spp. in <i>S. pastorianus</i> and for monitoring the fermentation of <i>S. pastorianus</i> through the quantitative detection of budding cells.https://www.mdpi.com/2079-6374/11/8/272resistive pulse methodyeastdiscriminationbrewer<i>Saccharomyces pastorianus</i><i>Dekkera</i> spp. |
| spellingShingle | Kazumichi Yokota Asae Takeo Hiroko Abe Yuji Kurokawa Muneaki Hashimoto Kazuaki Kajimoto Masato Tanaka Sanae Murayama Yoshihiro Nakajima Masateru Taniguchi Masatoshi Kataoka Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of <i>Saccharomyces pastorianus</i> from <i>Dekkera</i> spp. Biosensors resistive pulse method yeast discrimination brewer <i>Saccharomyces pastorianus</i> <i>Dekkera</i> spp. |
| title | Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of <i>Saccharomyces pastorianus</i> from <i>Dekkera</i> spp. |
| title_full | Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of <i>Saccharomyces pastorianus</i> from <i>Dekkera</i> spp. |
| title_fullStr | Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of <i>Saccharomyces pastorianus</i> from <i>Dekkera</i> spp. |
| title_full_unstemmed | Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of <i>Saccharomyces pastorianus</i> from <i>Dekkera</i> spp. |
| title_short | Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of <i>Saccharomyces pastorianus</i> from <i>Dekkera</i> spp. |
| title_sort | application of micropore device for accurate easy and rapid discrimination of i saccharomyces pastorianus i from i dekkera i spp |
| topic | resistive pulse method yeast discrimination brewer <i>Saccharomyces pastorianus</i> <i>Dekkera</i> spp. |
| url | https://www.mdpi.com/2079-6374/11/8/272 |
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