Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin
MYB superfamily is one of the most abundant families in plants, and plays critical role in plant growth, development, metabolism regulation, and stress response. Curcuma wenyujin is the main source plant of three traditional Chinese medicines, which are widely used in clinical treatment due to its d...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-04-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.894928/full |
| _version_ | 1818278793721151488 |
|---|---|
| author | Qiuhui Wei Qiuhui Wei Yuyang Liu Yuyang Liu Kaer Lan Kaer Lan Xin Wei Xin Wei Tianyuan Hu Tianyuan Hu Rong Chen Rong Chen Shujuan Zhao Shujuan Zhao Xiaopu Yin Xiaopu Yin Tian Xie Tian Xie |
| author_facet | Qiuhui Wei Qiuhui Wei Yuyang Liu Yuyang Liu Kaer Lan Kaer Lan Xin Wei Xin Wei Tianyuan Hu Tianyuan Hu Rong Chen Rong Chen Shujuan Zhao Shujuan Zhao Xiaopu Yin Xiaopu Yin Tian Xie Tian Xie |
| author_sort | Qiuhui Wei |
| collection | DOAJ |
| description | MYB superfamily is one of the most abundant families in plants, and plays critical role in plant growth, development, metabolism regulation, and stress response. Curcuma wenyujin is the main source plant of three traditional Chinese medicines, which are widely used in clinical treatment due to its diverse pharmacological activities. In present study, 88 CwMYBs were identified and analyzed in C. wenyujin, including 43 MYB-related genes, 42 R2R3-MYB genes, two 3R-MYB genes, and one 4R-MYB gene. Forty-three MYB-related proteins were classified into several types based on conserved domains and specific motifs, including CCA1-like type, R-R type, Myb-CC type, GARP-like type, and TBR-like type. The analysis of motifs in MYB DBD and no-MYB regions revealed the relevance of protein structure and function. Comparative phylogeny analysis divided 42 R2R3-MYB proteins into 19 subgroups and provided a reference for understanding the functions of some CwMYBs based on orthologs of previously characterized MYBs. Expression profile analysis of CwMYB genes revealed the differentially expressed genes responding to various abiotic stresses. Four candidate MYB genes were identified by combining the results of phylogeny analysis and expression analysis. CwMYB10, CwMYB18, CwMYB39, and CwMYB41 were significantly induced by cold, NaCl, and MeJA stress treatments. CwMYB18 and CwMYB41 were proved as regulators with activity of transcriptional activation, whereas CwMYB39 and CwMYB10 were not. They may participate in the response to abiotic stresses through different mechanisms in C. wenyujin. This study was the first step toward understanding the CwMYB family and the response to abiotic stresses in C. wenyujin. |
| first_indexed | 2024-12-12T23:23:05Z |
| format | Article |
| id | doaj.art-707ee77267c14f5eb6b2ff33f5017554 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-12T23:23:05Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-707ee77267c14f5eb6b2ff33f50175542022-12-22T00:08:13ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-04-011310.3389/fgene.2022.894928894928Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujinQiuhui Wei0Qiuhui Wei1Yuyang Liu2Yuyang Liu3Kaer Lan4Kaer Lan5Xin Wei6Xin Wei7Tianyuan Hu8Tianyuan Hu9Rong Chen10Rong Chen11Shujuan Zhao12Shujuan Zhao13Xiaopu Yin14Xiaopu Yin15Tian Xie16Tian Xie17School of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, ChinaMYB superfamily is one of the most abundant families in plants, and plays critical role in plant growth, development, metabolism regulation, and stress response. Curcuma wenyujin is the main source plant of three traditional Chinese medicines, which are widely used in clinical treatment due to its diverse pharmacological activities. In present study, 88 CwMYBs were identified and analyzed in C. wenyujin, including 43 MYB-related genes, 42 R2R3-MYB genes, two 3R-MYB genes, and one 4R-MYB gene. Forty-three MYB-related proteins were classified into several types based on conserved domains and specific motifs, including CCA1-like type, R-R type, Myb-CC type, GARP-like type, and TBR-like type. The analysis of motifs in MYB DBD and no-MYB regions revealed the relevance of protein structure and function. Comparative phylogeny analysis divided 42 R2R3-MYB proteins into 19 subgroups and provided a reference for understanding the functions of some CwMYBs based on orthologs of previously characterized MYBs. Expression profile analysis of CwMYB genes revealed the differentially expressed genes responding to various abiotic stresses. Four candidate MYB genes were identified by combining the results of phylogeny analysis and expression analysis. CwMYB10, CwMYB18, CwMYB39, and CwMYB41 were significantly induced by cold, NaCl, and MeJA stress treatments. CwMYB18 and CwMYB41 were proved as regulators with activity of transcriptional activation, whereas CwMYB39 and CwMYB10 were not. They may participate in the response to abiotic stresses through different mechanisms in C. wenyujin. This study was the first step toward understanding the CwMYB family and the response to abiotic stresses in C. wenyujin.https://www.frontiersin.org/articles/10.3389/fgene.2022.894928/fullMYB familyphylogeny analysisexpression patterntranscriptional activationC. wenyujin |
| spellingShingle | Qiuhui Wei Qiuhui Wei Yuyang Liu Yuyang Liu Kaer Lan Kaer Lan Xin Wei Xin Wei Tianyuan Hu Tianyuan Hu Rong Chen Rong Chen Shujuan Zhao Shujuan Zhao Xiaopu Yin Xiaopu Yin Tian Xie Tian Xie Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin Frontiers in Genetics MYB family phylogeny analysis expression pattern transcriptional activation C. wenyujin |
| title | Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin |
| title_full | Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin |
| title_fullStr | Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin |
| title_full_unstemmed | Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin |
| title_short | Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin |
| title_sort | identification and analysis of myb gene family for discovering potential regulators responding to abiotic stresses in curcuma wenyujin |
| topic | MYB family phylogeny analysis expression pattern transcriptional activation C. wenyujin |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.894928/full |
| work_keys_str_mv | AT qiuhuiwei identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT qiuhuiwei identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT yuyangliu identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT yuyangliu identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT kaerlan identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT kaerlan identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT xinwei identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT xinwei identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT tianyuanhu identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT tianyuanhu identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT rongchen identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT rongchen identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT shujuanzhao identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT shujuanzhao identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT xiaopuyin identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT xiaopuyin identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT tianxie identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin AT tianxie identificationandanalysisofmybgenefamilyfordiscoveringpotentialregulatorsrespondingtoabioticstressesincurcumawenyujin |