Intra-articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis
Abstract Background Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. We evaluated the safety and preliminary efficacy of administering SVF and PRP intra-articularly into patie...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-06-01
|
| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-017-1242-4 |
| _version_ | 1818386082000011264 |
|---|---|
| author | Himanshu Bansal Kristin Comella Jerry Leon Poonam Verma Diwaker Agrawal Prasad Koka Thomas Ichim |
| author_facet | Himanshu Bansal Kristin Comella Jerry Leon Poonam Verma Diwaker Agrawal Prasad Koka Thomas Ichim |
| author_sort | Himanshu Bansal |
| collection | DOAJ |
| description | Abstract Background Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. We evaluated the safety and preliminary efficacy of administering SVF and PRP intra-articularly into patients with osteoarthritis grade 1 and 2. Methods A total of ten patients underwent a local tumescent liposuction procedure to remove approximately 100 ml of fat tissue from the abdomen. SVF was isolated using an enzyme digestion and resuspended in PRP for intra-articular injection in the knee. The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score and six-minute walk distance (6MWD) were used to evaluate clinical effects and included measure of patient’s subjective assessment of pain, joint mobility, and physical disability. WOMAC score, 6MWD and laboratory tests were repeated at 3 and 6 months and 1, 1.5 and 2 years. XRAY and MRI were completed at 1 year. Results The average total WOMAC score was 64 at baseline and significantly reduced to 52 at 3 months, 46 at 6 months, 42 at 1 year, 38 at 1.5 years, and 41 at 2 years. Patients walked an average of 1310 feet at baseline and demonstrated a statistically significant improvement at 3 and 6 months and 1, 1.5, and 2 years post treatment. Cartilage thickness as determined by MRI improved by at least 0.2 mm in six patients, was unchanged in two patients and decreased by at least 0.2 mm in two patients. Conclusions Overall, all of the patients were pleased with the treatment results. They reported a reduction in pain levels, especially after 3 months. More importantly, the procedure demonstrated a strong safety profile with no severe adverse events or complications reported. Trial registration NCT03089762; Name of registry: http://www.clinicaltrials.gov |
| first_indexed | 2024-12-14T03:48:23Z |
| format | Article |
| id | doaj.art-7082cb8ce3e94b40865bf77a1223fefc |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-14T03:48:23Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-7082cb8ce3e94b40865bf77a1223fefc2022-12-21T23:18:17ZengBMCJournal of Translational Medicine1479-58762017-06-0115111110.1186/s12967-017-1242-4Intra-articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritisHimanshu Bansal0Kristin Comella1Jerry Leon2Poonam Verma3Diwaker Agrawal4Prasad Koka5Thomas Ichim6Mother Cell Spinal Injury & Stem Cell Research, Anupam HospitalUS Stem Cell, IncAdvance Health Institute MayaguezRegennMed Research and Therapeutics LLPMercy Medical CentreDepartment of Virology and Immunology, Haffkine InstituteRegenerative Medicine InstituteAbstract Background Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. We evaluated the safety and preliminary efficacy of administering SVF and PRP intra-articularly into patients with osteoarthritis grade 1 and 2. Methods A total of ten patients underwent a local tumescent liposuction procedure to remove approximately 100 ml of fat tissue from the abdomen. SVF was isolated using an enzyme digestion and resuspended in PRP for intra-articular injection in the knee. The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score and six-minute walk distance (6MWD) were used to evaluate clinical effects and included measure of patient’s subjective assessment of pain, joint mobility, and physical disability. WOMAC score, 6MWD and laboratory tests were repeated at 3 and 6 months and 1, 1.5 and 2 years. XRAY and MRI were completed at 1 year. Results The average total WOMAC score was 64 at baseline and significantly reduced to 52 at 3 months, 46 at 6 months, 42 at 1 year, 38 at 1.5 years, and 41 at 2 years. Patients walked an average of 1310 feet at baseline and demonstrated a statistically significant improvement at 3 and 6 months and 1, 1.5, and 2 years post treatment. Cartilage thickness as determined by MRI improved by at least 0.2 mm in six patients, was unchanged in two patients and decreased by at least 0.2 mm in two patients. Conclusions Overall, all of the patients were pleased with the treatment results. They reported a reduction in pain levels, especially after 3 months. More importantly, the procedure demonstrated a strong safety profile with no severe adverse events or complications reported. Trial registration NCT03089762; Name of registry: http://www.clinicaltrials.govhttp://link.springer.com/article/10.1186/s12967-017-1242-4Stromal vascular fraction (SVF)Adipose derived stromal/stem cells (ADSCs)Stem cellsAdipose tissueConnective tissueOsteoarthritis |
| spellingShingle | Himanshu Bansal Kristin Comella Jerry Leon Poonam Verma Diwaker Agrawal Prasad Koka Thomas Ichim Intra-articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis Journal of Translational Medicine Stromal vascular fraction (SVF) Adipose derived stromal/stem cells (ADSCs) Stem cells Adipose tissue Connective tissue Osteoarthritis |
| title | Intra-articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis |
| title_full | Intra-articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis |
| title_fullStr | Intra-articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis |
| title_full_unstemmed | Intra-articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis |
| title_short | Intra-articular injection in the knee of adipose derived stromal cells (stromal vascular fraction) and platelet rich plasma for osteoarthritis |
| title_sort | intra articular injection in the knee of adipose derived stromal cells stromal vascular fraction and platelet rich plasma for osteoarthritis |
| topic | Stromal vascular fraction (SVF) Adipose derived stromal/stem cells (ADSCs) Stem cells Adipose tissue Connective tissue Osteoarthritis |
| url | http://link.springer.com/article/10.1186/s12967-017-1242-4 |
| work_keys_str_mv | AT himanshubansal intraarticularinjectioninthekneeofadiposederivedstromalcellsstromalvascularfractionandplateletrichplasmaforosteoarthritis AT kristincomella intraarticularinjectioninthekneeofadiposederivedstromalcellsstromalvascularfractionandplateletrichplasmaforosteoarthritis AT jerryleon intraarticularinjectioninthekneeofadiposederivedstromalcellsstromalvascularfractionandplateletrichplasmaforosteoarthritis AT poonamverma intraarticularinjectioninthekneeofadiposederivedstromalcellsstromalvascularfractionandplateletrichplasmaforosteoarthritis AT diwakeragrawal intraarticularinjectioninthekneeofadiposederivedstromalcellsstromalvascularfractionandplateletrichplasmaforosteoarthritis AT prasadkoka intraarticularinjectioninthekneeofadiposederivedstromalcellsstromalvascularfractionandplateletrichplasmaforosteoarthritis AT thomasichim intraarticularinjectioninthekneeofadiposederivedstromalcellsstromalvascularfractionandplateletrichplasmaforosteoarthritis |