RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS
Polyhydroxyalkanoates (PHAs) are intracellular material accumulated by several bacteria. Commercial production of PHAs faces the issue of high production cost especially substrate cost and recovery/separation cost. An alternative to reduce the production cost is to use enzyme and or chemical to reco...
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| Format: | Article |
| Language: | English |
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Diponegoro University
2006-11-01
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| Series: | Reaktor |
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| Online Access: | https://ejournal.undip.ac.id/index.php/reaktor/article/view/1487 |
| _version_ | 1819266227224182784 |
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| author | S. Marsudi |
| author_facet | S. Marsudi |
| author_sort | S. Marsudi |
| collection | DOAJ |
| description | Polyhydroxyalkanoates (PHAs) are intracellular material accumulated by several bacteria. Commercial production of PHAs faces the issue of high production cost especially substrate cost and recovery/separation cost. An alternative to reduce the production cost is to use enzyme and or chemical to recover PHAs from bacterial cells. Recovery of PHAs from bacterial cells was done using enzyme, chemical, and a mixture of enzyme and chemical. Enzyme (s) and or chemical(s) were added into culture broth to disrupt cells after adjusting pH and temperature of the culture broth. Treatment by adding enzyme or chemical only into culture broth showed a low level of PHAs recovered from bacterial cells. Treatment by adding a mixture of enzymes and chemicals showed the best result among 22 examined combinations, i.e. a mixture of EDTA, lisozyme, papain enzyme, and SDS. This combination gave a PHA recovery of 65 % w/w. |
| first_indexed | 2024-12-23T20:57:55Z |
| format | Article |
| id | doaj.art-7084ec9be8954ddc8cdcbd71f79bd129 |
| institution | Directory Open Access Journal |
| issn | 0852-0798 2407-5973 |
| language | English |
| last_indexed | 2024-12-23T20:57:55Z |
| publishDate | 2006-11-01 |
| publisher | Diponegoro University |
| record_format | Article |
| series | Reaktor |
| spelling | doaj.art-7084ec9be8954ddc8cdcbd71f79bd1292022-12-21T17:31:27ZengDiponegoro UniversityReaktor0852-07982407-59732006-11-01102596210.14710/reaktor.10.2.59-621181RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESSS. Marsudi0Departemen Teknik Kimia, Fakultas Teknologi Industri, Institut Teknologi Indonesia Jalan Raya Puspiptek - Serpong Tangerang 15311 Telp. (021) 7561092Polyhydroxyalkanoates (PHAs) are intracellular material accumulated by several bacteria. Commercial production of PHAs faces the issue of high production cost especially substrate cost and recovery/separation cost. An alternative to reduce the production cost is to use enzyme and or chemical to recover PHAs from bacterial cells. Recovery of PHAs from bacterial cells was done using enzyme, chemical, and a mixture of enzyme and chemical. Enzyme (s) and or chemical(s) were added into culture broth to disrupt cells after adjusting pH and temperature of the culture broth. Treatment by adding enzyme or chemical only into culture broth showed a low level of PHAs recovered from bacterial cells. Treatment by adding a mixture of enzymes and chemicals showed the best result among 22 examined combinations, i.e. a mixture of EDTA, lisozyme, papain enzyme, and SDS. This combination gave a PHA recovery of 65 % w/w.https://ejournal.undip.ac.id/index.php/reaktor/article/view/1487culture brothenzymatic processrecovery of PHAs |
| spellingShingle | S. Marsudi RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS Reaktor culture broth enzymatic process recovery of PHAs |
| title | RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS |
| title_full | RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS |
| title_fullStr | RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS |
| title_full_unstemmed | RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS |
| title_short | RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS |
| title_sort | recovery of polyhydroxyalkanoates phas from bacterial cells using enzymatic process |
| topic | culture broth enzymatic process recovery of PHAs |
| url | https://ejournal.undip.ac.id/index.php/reaktor/article/view/1487 |
| work_keys_str_mv | AT smarsudi recoveryofpolyhydroxyalkanoatesphasfrombacterialcellsusingenzymaticprocess |