Loading of green-synthesized cu nanoparticles on Ag complex containing 1,3,5-triazine Schiff base with enhanced antimicrobial activities
Abstract The physicochemical properties of materials change significantly in nanometer dimensions. Therefore, several methods have been proposed for the synthesis of nanoparticles. Plant extracts and essential oils are applied as natural and economic resources to prepare nanomaterials especially met...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47358-4 |
| _version_ | 1797453066954342400 |
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| author | Elham Pormohammad Pouya Ghamari kargar Ghodsieh Bagherzade Hamid Beyzaei |
| author_facet | Elham Pormohammad Pouya Ghamari kargar Ghodsieh Bagherzade Hamid Beyzaei |
| author_sort | Elham Pormohammad |
| collection | DOAJ |
| description | Abstract The physicochemical properties of materials change significantly in nanometer dimensions. Therefore, several methods have been proposed for the synthesis of nanoparticles. Plant extracts and essential oils are applied as natural and economic resources to prepare nanomaterials especially metal nanoparticles. In this project, a green, simple and efficient method has been designed for the synthesis of Cu nanoparticles using Purple cabbage extract as a reducing and stabilizing agent. They were successfully loaded onto a new Ag complex containing 1,3,5-triazine Schiff base as ligand to form Cu@Ag-CPX nanocomposite. Phytochemical contents of extract were identified by standard qualitative analyses. The chemical structure of all synthesized compounds was characterized using spectral data. In FT-IR, coordination of C=N bond of Schiff base ligand to Ag+ ions shifted the absorption band from 1641 to 1632 cm−1. The UV–Vis spectrum of Cu@Ag-CPX nanocomposite shown the peak related to Cu nanoparticles in the region of around 251 nm. 5:7 molar ratio of Cu to Ag in Cu@Ag-CPX was determined using ICP-OES. The FESEM, TEM, and DLS techniques provided valuable insights into the morphology and size distribution of the nanocomposite, revealing the presence of rods and monodispersed particles with specific diameter ranges. These analyses of the nanocomposite displayed rods with diameters from 40 to 62 nm as well as monodispersed and uniform particles with average diameter of 45 nm, respectively. The presence of elements including carbon, nitrogen, oxygen, Cu and Ag was proved by EDX-EDS analysis. The XRD pattern of Cu@Ag-CPX shown the diffraction peaks of Cu and Ag particles at 2θ values of 10°–80°, and confirmed its crystalline nature. The inhibitory properties of the synthesized compounds were evaluated in vitro against four Gram-negative and two Gram-positive bacteria, as well as two fungal strains. The MIC, MBC and MFC values obtained from microdilution and streak plate sensitivity tests were ranged from 128 to 4096 µg ml−1. While Cu nanoparticles and Ag complexes were effective against some pathogens, they were not effective against all them. However, the growth of all tested microbial strains was inhibited by Cu@Ag-CPX nanocomposite, and makes it as a new promising antimicrobial agent. Modification of nanocomposite in terms of nanoparticle and complex can improve its blocking activities. |
| first_indexed | 2024-03-09T15:17:35Z |
| format | Article |
| id | doaj.art-70878027bc8a4d6cbc6cb6c4f0c3f05b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:17:35Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-70878027bc8a4d6cbc6cb6c4f0c3f05b2023-11-26T12:59:03ZengNature PortfolioScientific Reports2045-23222023-11-0113111210.1038/s41598-023-47358-4Loading of green-synthesized cu nanoparticles on Ag complex containing 1,3,5-triazine Schiff base with enhanced antimicrobial activitiesElham Pormohammad0Pouya Ghamari kargar1Ghodsieh Bagherzade2Hamid Beyzaei3Department of Chemistry, Faculty of Sciences, University of BirjandDepartment of Chemistry, Faculty of Sciences, University of BirjandDepartment of Chemistry, Faculty of Sciences, University of BirjandDepartment of Chemistry, Faculty of Science, University of ZabolAbstract The physicochemical properties of materials change significantly in nanometer dimensions. Therefore, several methods have been proposed for the synthesis of nanoparticles. Plant extracts and essential oils are applied as natural and economic resources to prepare nanomaterials especially metal nanoparticles. In this project, a green, simple and efficient method has been designed for the synthesis of Cu nanoparticles using Purple cabbage extract as a reducing and stabilizing agent. They were successfully loaded onto a new Ag complex containing 1,3,5-triazine Schiff base as ligand to form Cu@Ag-CPX nanocomposite. Phytochemical contents of extract were identified by standard qualitative analyses. The chemical structure of all synthesized compounds was characterized using spectral data. In FT-IR, coordination of C=N bond of Schiff base ligand to Ag+ ions shifted the absorption band from 1641 to 1632 cm−1. The UV–Vis spectrum of Cu@Ag-CPX nanocomposite shown the peak related to Cu nanoparticles in the region of around 251 nm. 5:7 molar ratio of Cu to Ag in Cu@Ag-CPX was determined using ICP-OES. The FESEM, TEM, and DLS techniques provided valuable insights into the morphology and size distribution of the nanocomposite, revealing the presence of rods and monodispersed particles with specific diameter ranges. These analyses of the nanocomposite displayed rods with diameters from 40 to 62 nm as well as monodispersed and uniform particles with average diameter of 45 nm, respectively. The presence of elements including carbon, nitrogen, oxygen, Cu and Ag was proved by EDX-EDS analysis. The XRD pattern of Cu@Ag-CPX shown the diffraction peaks of Cu and Ag particles at 2θ values of 10°–80°, and confirmed its crystalline nature. The inhibitory properties of the synthesized compounds were evaluated in vitro against four Gram-negative and two Gram-positive bacteria, as well as two fungal strains. The MIC, MBC and MFC values obtained from microdilution and streak plate sensitivity tests were ranged from 128 to 4096 µg ml−1. While Cu nanoparticles and Ag complexes were effective against some pathogens, they were not effective against all them. However, the growth of all tested microbial strains was inhibited by Cu@Ag-CPX nanocomposite, and makes it as a new promising antimicrobial agent. Modification of nanocomposite in terms of nanoparticle and complex can improve its blocking activities.https://doi.org/10.1038/s41598-023-47358-4 |
| spellingShingle | Elham Pormohammad Pouya Ghamari kargar Ghodsieh Bagherzade Hamid Beyzaei Loading of green-synthesized cu nanoparticles on Ag complex containing 1,3,5-triazine Schiff base with enhanced antimicrobial activities Scientific Reports |
| title | Loading of green-synthesized cu nanoparticles on Ag complex containing 1,3,5-triazine Schiff base with enhanced antimicrobial activities |
| title_full | Loading of green-synthesized cu nanoparticles on Ag complex containing 1,3,5-triazine Schiff base with enhanced antimicrobial activities |
| title_fullStr | Loading of green-synthesized cu nanoparticles on Ag complex containing 1,3,5-triazine Schiff base with enhanced antimicrobial activities |
| title_full_unstemmed | Loading of green-synthesized cu nanoparticles on Ag complex containing 1,3,5-triazine Schiff base with enhanced antimicrobial activities |
| title_short | Loading of green-synthesized cu nanoparticles on Ag complex containing 1,3,5-triazine Schiff base with enhanced antimicrobial activities |
| title_sort | loading of green synthesized cu nanoparticles on ag complex containing 1 3 5 triazine schiff base with enhanced antimicrobial activities |
| url | https://doi.org/10.1038/s41598-023-47358-4 |
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