Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF
Summary: The mammalian brain is organized over sizes that span several orders of magnitude, from synapses to the entire brain. Thus, a technique to visualize neural circuits across multiple spatial scales (multi-scale neuronal imaging) is vital for deciphering brain-wide connectivity. Here, we devel...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221015716 |
| _version_ | 1819261378539552768 |
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| author | Takahiro Furuta Kenta Yamauchi Shinichiro Okamoto Megumu Takahashi Soichiro Kakuta Yoko Ishida Aya Takenaka Atsushi Yoshida Yasuo Uchiyama Masato Koike Kaoru Isa Tadashi Isa Hiroyuki Hioki |
| author_facet | Takahiro Furuta Kenta Yamauchi Shinichiro Okamoto Megumu Takahashi Soichiro Kakuta Yoko Ishida Aya Takenaka Atsushi Yoshida Yasuo Uchiyama Masato Koike Kaoru Isa Tadashi Isa Hiroyuki Hioki |
| author_sort | Takahiro Furuta |
| collection | DOAJ |
| description | Summary: The mammalian brain is organized over sizes that span several orders of magnitude, from synapses to the entire brain. Thus, a technique to visualize neural circuits across multiple spatial scales (multi-scale neuronal imaging) is vital for deciphering brain-wide connectivity. Here, we developed this technique by coupling successive light microscopy/electron microscopy (LM/EM) imaging with a glutaraldehyde-resistant tissue clearing method, ScaleSF. Our multi-scale neuronal imaging incorporates (1) brain-wide macroscopic observation, (2) mesoscopic circuit mapping, (3) microscopic subcellular imaging, and (4) EM imaging of nanoscopic structures, allowing seamless integration of structural information from the brain to synapses. We applied this technique to three neural circuits of two different species, mouse striatofugal, mouse callosal, and marmoset corticostriatal projection systems, and succeeded in simultaneous interrogation of their circuit structure and synaptic connectivity in a targeted way. Our multi-scale neuronal imaging will significantly advance the understanding of brain-wide connectivity by expanding the scales of objects. |
| first_indexed | 2024-12-23T19:40:51Z |
| format | Article |
| id | doaj.art-708868f67aef4da0999575f7cce6ba6e |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-23T19:40:51Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-708868f67aef4da0999575f7cce6ba6e2022-12-21T17:33:40ZengElsevieriScience2589-00422022-01-01251103601Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSFTakahiro Furuta0Kenta Yamauchi1Shinichiro Okamoto2Megumu Takahashi3Soichiro Kakuta4Yoko Ishida5Aya Takenaka6Atsushi Yoshida7Yasuo Uchiyama8Masato Koike9Kaoru Isa10Tadashi Isa11Hiroyuki Hioki12Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, JapanDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Advanced Research Institute for Health Sciences, Juntendo University, Bunkyo-Ku, Tokyo 113-8421, JapanDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Advanced Research Institute for Health Sciences, Juntendo University, Bunkyo-Ku, Tokyo 113-8421, JapanDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Neuroscience, Graduate School of Medicine, Kyoto University, Kyoto, Kyoto 606-8501, JapanLaboratory of Morphology and Image Analysis, Biomedical Research Core Facilities, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, JapanDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Advanced Research Institute for Health Sciences, Juntendo University, Bunkyo-Ku, Tokyo 113-8421, JapanDepartment of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, JapanDepartment of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, JapanDepartment of Cellular and Molecular Neuropathology, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, JapanDepartment of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Advanced Research Institute for Health Sciences, Juntendo University, Bunkyo-Ku, Tokyo 113-8421, JapanDepartment of Neuroscience, Graduate School of Medicine, Kyoto University, Kyoto, Kyoto 606-8501, JapanDepartment of Neuroscience, Graduate School of Medicine, Kyoto University, Kyoto, Kyoto 606-8501, Japan; Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto, Kyoto 606-8501, JapanDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Corresponding authorSummary: The mammalian brain is organized over sizes that span several orders of magnitude, from synapses to the entire brain. Thus, a technique to visualize neural circuits across multiple spatial scales (multi-scale neuronal imaging) is vital for deciphering brain-wide connectivity. Here, we developed this technique by coupling successive light microscopy/electron microscopy (LM/EM) imaging with a glutaraldehyde-resistant tissue clearing method, ScaleSF. Our multi-scale neuronal imaging incorporates (1) brain-wide macroscopic observation, (2) mesoscopic circuit mapping, (3) microscopic subcellular imaging, and (4) EM imaging of nanoscopic structures, allowing seamless integration of structural information from the brain to synapses. We applied this technique to three neural circuits of two different species, mouse striatofugal, mouse callosal, and marmoset corticostriatal projection systems, and succeeded in simultaneous interrogation of their circuit structure and synaptic connectivity in a targeted way. Our multi-scale neuronal imaging will significantly advance the understanding of brain-wide connectivity by expanding the scales of objects.http://www.sciencedirect.com/science/article/pii/S2589004221015716Imaging anatomyOptical imagingNeuroscienceCell biology |
| spellingShingle | Takahiro Furuta Kenta Yamauchi Shinichiro Okamoto Megumu Takahashi Soichiro Kakuta Yoko Ishida Aya Takenaka Atsushi Yoshida Yasuo Uchiyama Masato Koike Kaoru Isa Tadashi Isa Hiroyuki Hioki Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF iScience Imaging anatomy Optical imaging Neuroscience Cell biology |
| title | Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF |
| title_full | Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF |
| title_fullStr | Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF |
| title_full_unstemmed | Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF |
| title_short | Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF |
| title_sort | multi scale light microscopy electron microscopy neuronal imaging from brain to synapse with a tissue clearing method scalesf |
| topic | Imaging anatomy Optical imaging Neuroscience Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221015716 |
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