Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping
The v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were i...
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MDPI AG
2015-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/16/7/15009 |
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| author | Fengqing Wang Yanfei Suo He Wei Mingjie Li Caixia Xie Lina Wang Xinjian Chen Zhongyi Zhang |
| author_facet | Fengqing Wang Yanfei Suo He Wei Mingjie Li Caixia Xie Lina Wang Xinjian Chen Zhongyi Zhang |
| author_sort | Fengqing Wang |
| collection | DOAJ |
| description | The v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were isolated. These 40 genes were grouped into five categories, one R1R2R3-MYB, four TRFL MYBs, four SMH MYBs, 25 R2R3-MYBs, and six MYB-related members. The MYB DNA-binding domain (DBD) sequence composition was conserved among proteins of the same subgroup. As expected, most of the closely related members in the phylogenetic tree exhibited common motifs. Additionally, the gene structure and motifs of the R. glutinosa MYB genes were analyzed. MYB gene expression was analyzed in the leaf and the tuberous root under two abiotic stress conditions. Expression profiles showed that most R. glutinosa MYB genes were expressed in the leaf and the tuberous root, suggesting that MYB genes are involved in various physiological and developmental processes in R. glutinosa. Seven MYB genes were up-regulated in response to shading in at least one tissue. Two MYB genes showed increased expression and 13 MYB genes showed decreased expression in the tuberous root under continuous cropping. This investigation is the first comprehensive study of the MYB gene family in R. glutinosa. |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T10:30:37Z |
| publishDate | 2015-07-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7089e2af64c342c2a3f08d6318b10dc42022-12-22T02:50:11ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-07-01167150091503010.3390/ijms160715009ijms160715009Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous CroppingFengqing Wang0Yanfei Suo1He Wei2Mingjie Li3Caixia Xie4Lina Wang5Xinjian Chen6Zhongyi Zhang7College of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaInstitute of Industrial Crops, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaCollege of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaSchool of medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, ChinaSchool of medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450002, ChinaThe v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were isolated. These 40 genes were grouped into five categories, one R1R2R3-MYB, four TRFL MYBs, four SMH MYBs, 25 R2R3-MYBs, and six MYB-related members. The MYB DNA-binding domain (DBD) sequence composition was conserved among proteins of the same subgroup. As expected, most of the closely related members in the phylogenetic tree exhibited common motifs. Additionally, the gene structure and motifs of the R. glutinosa MYB genes were analyzed. MYB gene expression was analyzed in the leaf and the tuberous root under two abiotic stress conditions. Expression profiles showed that most R. glutinosa MYB genes were expressed in the leaf and the tuberous root, suggesting that MYB genes are involved in various physiological and developmental processes in R. glutinosa. Seven MYB genes were up-regulated in response to shading in at least one tissue. Two MYB genes showed increased expression and 13 MYB genes showed decreased expression in the tuberous root under continuous cropping. This investigation is the first comprehensive study of the MYB gene family in R. glutinosa.http://www.mdpi.com/1422-0067/16/7/15009MYB transcription factorshadingcontinuous croppingRehmannia glutinosa |
| spellingShingle | Fengqing Wang Yanfei Suo He Wei Mingjie Li Caixia Xie Lina Wang Xinjian Chen Zhongyi Zhang Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping International Journal of Molecular Sciences MYB transcription factor shading continuous cropping Rehmannia glutinosa |
| title | Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping |
| title_full | Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping |
| title_fullStr | Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping |
| title_full_unstemmed | Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping |
| title_short | Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping |
| title_sort | identification and characterization of 40 isolated rehmannia glutinosa myb family genes and their expression profiles in response to shading and continuous cropping |
| topic | MYB transcription factor shading continuous cropping Rehmannia glutinosa |
| url | http://www.mdpi.com/1422-0067/16/7/15009 |
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