Evaluation of the red cell hemolysis in packed red cells during processing and storage
<b>Introduction</b> : Storage of red cells causes a progressive increase in hemolysis. In spite of the use of additive solutions for storage and filters for leucoreduction, some amount of hemolysis is still inevitable. The extent of hemolysis, however, should not exceed the permissible t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2011-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=1;spage=15;epage=17;aulast=Makroo |
| _version_ | 1818989842702270464 |
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| author | Makroo R Raina Vimarsh Bhatia Aakanksha Gupta Richa Majid Abdul Thakur Uday Rosamma N |
| author_facet | Makroo R Raina Vimarsh Bhatia Aakanksha Gupta Richa Majid Abdul Thakur Uday Rosamma N |
| author_sort | Makroo R |
| collection | DOAJ |
| description | <b>Introduction</b> : Storage of red cells causes a progressive increase in hemolysis. In spite of the use of additive solutions for storage and filters for leucoreduction, some amount of hemolysis is still inevitable. The extent of hemolysis, however, should not exceed the permissible threshold for hemolysis even on the 42 <sup>nd</sup> day of storage. <b>Study Design and Methods:</b> Eighty units of packed red cells, 40 stored in SAGM post leucoreduction and 40 in ADSOL without leucoreduction filters, were evaluated for plasma hemoglobin by HemoCue Plasma Hemoglobin analyzer on the day of collection and on the 7 <sup>th</sup> , 14 <sup>th</sup> , 21 <sup>st</sup> , 28 <sup>th</sup> , 35 <sup>th</sup> and 42 <sup>nd</sup> days thereafter. The hemoglobin and hematocrit were also noted for all these units by the Beckman and Coulter analyzer. Percentage hemolysis was then calculated. <b>Observations:</b> Hemolysis progressively increased with the storage period in all the stored red cell units (SAGM as well ADSOL). However, on day 42 <sup>nd</sup> of storage, free hemoglobin in all the red cell units was within the permissible level (which is 0.8% according to the Council of Europe guidelines and 1% as per the US FDA guidelines). The mean percentage hemolysis was slightly higher in the SAGM-containing bags with an integral leucoreduction filter as compared to the bags containing ADSOL. However this difference was marginal and not statistically significant. <b>Conclusion:</b> Hemolysis of the red cells increases with storage. However, maximum hemolysis does not exceed the permissible limits at any time thereby indicating the effect of optimum processing and storage conditions on red cell hemolysis. |
| first_indexed | 2024-12-20T19:44:54Z |
| format | Article |
| id | doaj.art-708d51df93904e21a455ef0a66d5bbd5 |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-12-20T19:44:54Z |
| publishDate | 2011-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-708d51df93904e21a455ef0a66d5bbd52022-12-21T19:28:26ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652011-01-01511517Evaluation of the red cell hemolysis in packed red cells during processing and storageMakroo RRaina VimarshBhatia AakankshaGupta RichaMajid AbdulThakur UdayRosamma N<b>Introduction</b> : Storage of red cells causes a progressive increase in hemolysis. In spite of the use of additive solutions for storage and filters for leucoreduction, some amount of hemolysis is still inevitable. The extent of hemolysis, however, should not exceed the permissible threshold for hemolysis even on the 42 <sup>nd</sup> day of storage. <b>Study Design and Methods:</b> Eighty units of packed red cells, 40 stored in SAGM post leucoreduction and 40 in ADSOL without leucoreduction filters, were evaluated for plasma hemoglobin by HemoCue Plasma Hemoglobin analyzer on the day of collection and on the 7 <sup>th</sup> , 14 <sup>th</sup> , 21 <sup>st</sup> , 28 <sup>th</sup> , 35 <sup>th</sup> and 42 <sup>nd</sup> days thereafter. The hemoglobin and hematocrit were also noted for all these units by the Beckman and Coulter analyzer. Percentage hemolysis was then calculated. <b>Observations:</b> Hemolysis progressively increased with the storage period in all the stored red cell units (SAGM as well ADSOL). However, on day 42 <sup>nd</sup> of storage, free hemoglobin in all the red cell units was within the permissible level (which is 0.8% according to the Council of Europe guidelines and 1% as per the US FDA guidelines). The mean percentage hemolysis was slightly higher in the SAGM-containing bags with an integral leucoreduction filter as compared to the bags containing ADSOL. However this difference was marginal and not statistically significant. <b>Conclusion:</b> Hemolysis of the red cells increases with storage. However, maximum hemolysis does not exceed the permissible limits at any time thereby indicating the effect of optimum processing and storage conditions on red cell hemolysis.http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=1;spage=15;epage=17;aulast=MakrooAdditive solutionshematocrithemolysisleucoreduction |
| spellingShingle | Makroo R Raina Vimarsh Bhatia Aakanksha Gupta Richa Majid Abdul Thakur Uday Rosamma N Evaluation of the red cell hemolysis in packed red cells during processing and storage Asian Journal of Transfusion Science Additive solutions hematocrit hemolysis leucoreduction |
| title | Evaluation of the red cell hemolysis in packed red cells during processing and storage |
| title_full | Evaluation of the red cell hemolysis in packed red cells during processing and storage |
| title_fullStr | Evaluation of the red cell hemolysis in packed red cells during processing and storage |
| title_full_unstemmed | Evaluation of the red cell hemolysis in packed red cells during processing and storage |
| title_short | Evaluation of the red cell hemolysis in packed red cells during processing and storage |
| title_sort | evaluation of the red cell hemolysis in packed red cells during processing and storage |
| topic | Additive solutions hematocrit hemolysis leucoreduction |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=1;spage=15;epage=17;aulast=Makroo |
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