Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145
Objective To analyze the expression levels of miR-145 in ALL children and their effects on the prognosis of ALL and to explore the mechanism of miR-145 in reversing the resistance of ALL cells to glucocorticoids. Methods A GEO database dataset was used to analyze the expression levels of miR-145 in...
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PeerJ Inc.
2020-06-01
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| Online Access: | https://peerj.com/articles/9337.pdf |
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| author | Sili Long Danwei Ren Fangfang Zhong Yana Niu Xiang Qin Dan Mu Wenjun Liu |
| author_facet | Sili Long Danwei Ren Fangfang Zhong Yana Niu Xiang Qin Dan Mu Wenjun Liu |
| author_sort | Sili Long |
| collection | DOAJ |
| description | Objective To analyze the expression levels of miR-145 in ALL children and their effects on the prognosis of ALL and to explore the mechanism of miR-145 in reversing the resistance of ALL cells to glucocorticoids. Methods A GEO database dataset was used to analyze the expression levels of miR-145 in ALL children. The association between miR-145 and childhood prognosis was analyzed by the TARGET database data. The expression levels of miR-145 in the glucocorticoid-resistant ALL cell line CEM-C1 were increased by lipofectamine 2000-mediated transfection. Cell proliferation inhibition experiments were performed to detect the effect of miR-145 on the response of CEM-C1 cell line to glucocorticoids. The expression levels of the apoptotic, autophagic and drug resistance-associated genes and proteins were detected by qPCR and western blot analysis. Results The expression levels of miR-145 were decreased in ALL patients (P < 0.001) and the prognosis of ALL in children with high miR-145 expression was significantly improved (P < 0.001). Increased miR-145 expression can improve the sensitivity of CEM-C1 cells to glucocorticoids. The expression levels of the proapoptotic and the anti-apoptotic genes Bax and Bcl-2 were increased and decreased, respectively, whereas the expression levels of the autophagicgenes Beclin 1 and LC were increased. In addition, the expression levels of the drug resistance gene MDR1 were decreased. Conclusion The expression levels of miR-145 in ALL children were decreased and they were associated with disease prognosis. The data indicated that miR-145 can reverse cell resistance by regulating apoptosis of CEM-C1 cells and autophagy. |
| first_indexed | 2024-03-09T06:25:54Z |
| format | Article |
| id | doaj.art-708d861f0f514dc781cdca6a32a71dfb |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:25:54Z |
| publishDate | 2020-06-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-708d861f0f514dc781cdca6a32a71dfb2023-12-03T11:20:46ZengPeerJ Inc.PeerJ2167-83592020-06-018e933710.7717/peerj.9337Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145Sili LongDanwei RenFangfang ZhongYana NiuXiang QinDan MuWenjun LiuObjective To analyze the expression levels of miR-145 in ALL children and their effects on the prognosis of ALL and to explore the mechanism of miR-145 in reversing the resistance of ALL cells to glucocorticoids. Methods A GEO database dataset was used to analyze the expression levels of miR-145 in ALL children. The association between miR-145 and childhood prognosis was analyzed by the TARGET database data. The expression levels of miR-145 in the glucocorticoid-resistant ALL cell line CEM-C1 were increased by lipofectamine 2000-mediated transfection. Cell proliferation inhibition experiments were performed to detect the effect of miR-145 on the response of CEM-C1 cell line to glucocorticoids. The expression levels of the apoptotic, autophagic and drug resistance-associated genes and proteins were detected by qPCR and western blot analysis. Results The expression levels of miR-145 were decreased in ALL patients (P < 0.001) and the prognosis of ALL in children with high miR-145 expression was significantly improved (P < 0.001). Increased miR-145 expression can improve the sensitivity of CEM-C1 cells to glucocorticoids. The expression levels of the proapoptotic and the anti-apoptotic genes Bax and Bcl-2 were increased and decreased, respectively, whereas the expression levels of the autophagicgenes Beclin 1 and LC were increased. In addition, the expression levels of the drug resistance gene MDR1 were decreased. Conclusion The expression levels of miR-145 in ALL children were decreased and they were associated with disease prognosis. The data indicated that miR-145 can reverse cell resistance by regulating apoptosis of CEM-C1 cells and autophagy.https://peerj.com/articles/9337.pdfAcute lymphoblastic leukemiamiR-145ChildrenGlucocorticoidDrug resistance |
| spellingShingle | Sili Long Danwei Ren Fangfang Zhong Yana Niu Xiang Qin Dan Mu Wenjun Liu Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145 PeerJ Acute lymphoblastic leukemia miR-145 Children Glucocorticoid Drug resistance |
| title | Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145 |
| title_full | Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145 |
| title_fullStr | Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145 |
| title_full_unstemmed | Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145 |
| title_short | Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145 |
| title_sort | reversal of glucocorticoid resistance in acute lymphoblastic leukemia cells by mir 145 |
| topic | Acute lymphoblastic leukemia miR-145 Children Glucocorticoid Drug resistance |
| url | https://peerj.com/articles/9337.pdf |
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