In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite
Hydroxyapatite (HA) is an important component of the bone mineral phase. It has been used in several applications, such as bone regenerative medicine, tooth implants, drug delivery and oral care cosmetics. In the present study, three different batches of a commercial nanohydroxyapatite (nHA) materia...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/5/1152 |
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| author | Rafaela-Maria Kavasi Catarina C. Coelho Varvara Platania Paulo A. Quadros Maria Chatzinikolaidou |
| author_facet | Rafaela-Maria Kavasi Catarina C. Coelho Varvara Platania Paulo A. Quadros Maria Chatzinikolaidou |
| author_sort | Rafaela-Maria Kavasi |
| collection | DOAJ |
| description | Hydroxyapatite (HA) is an important component of the bone mineral phase. It has been used in several applications, such as bone regenerative medicine, tooth implants, drug delivery and oral care cosmetics. In the present study, three different batches of a commercial nanohydroxyapatite (nHA) material were physicochemically-characterized and biologically-evaluated by means of cytotoxicity and genotoxicity using appropriate cell lines based on well-established guidelines (ISO10993-5 and OECD 487). The nHAs were characterized for their size and morphology by dynamic light scattering (DLS) and transmission electron microscopy (TEM) and were found to have a rod-like shape with an average length of approximately 20 to 40 nm. The nanoparticles were cytocompatible according to ISO 10993-5, and the in vitro micronucleus assay showed no genotoxicity to cells. Internalization by MC3T3-E1 cells was observed by TEM images, with nHA identified only in the cytoplasm and extracellular space. This result also validates the genotoxicity since nHA was not observed in the nucleus. The internalization of nHA by the cells did not seem to affect normal cell behavior, since the results showed good biocompatibility of these nHA nanoparticles. Therefore, this work is a relevant contribution for the safety assessment of this nHA material. |
| first_indexed | 2024-03-10T11:52:07Z |
| format | Article |
| id | doaj.art-708ee7c973a04101ae8d3a3a6a9dc393 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T11:52:07Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-708ee7c973a04101ae8d3a3a6a9dc3932023-11-21T17:36:37ZengMDPI AGNanomaterials2079-49912021-04-01115115210.3390/nano11051152In Vitro Biocompatibility Assessment of Nano-HydroxyapatiteRafaela-Maria Kavasi0Catarina C. Coelho1Varvara Platania2Paulo A. Quadros3Maria Chatzinikolaidou4Department of Materials Science and Technology, University of Crete, 70013 Heraklion, GreeceFLUIDINOVA, S.A., 4470-605 Maia, PortugalDepartment of Materials Science and Technology, University of Crete, 70013 Heraklion, GreeceFLUIDINOVA, S.A., 4470-605 Maia, PortugalDepartment of Materials Science and Technology, University of Crete, 70013 Heraklion, GreeceHydroxyapatite (HA) is an important component of the bone mineral phase. It has been used in several applications, such as bone regenerative medicine, tooth implants, drug delivery and oral care cosmetics. In the present study, three different batches of a commercial nanohydroxyapatite (nHA) material were physicochemically-characterized and biologically-evaluated by means of cytotoxicity and genotoxicity using appropriate cell lines based on well-established guidelines (ISO10993-5 and OECD 487). The nHAs were characterized for their size and morphology by dynamic light scattering (DLS) and transmission electron microscopy (TEM) and were found to have a rod-like shape with an average length of approximately 20 to 40 nm. The nanoparticles were cytocompatible according to ISO 10993-5, and the in vitro micronucleus assay showed no genotoxicity to cells. Internalization by MC3T3-E1 cells was observed by TEM images, with nHA identified only in the cytoplasm and extracellular space. This result also validates the genotoxicity since nHA was not observed in the nucleus. The internalization of nHA by the cells did not seem to affect normal cell behavior, since the results showed good biocompatibility of these nHA nanoparticles. Therefore, this work is a relevant contribution for the safety assessment of this nHA material.https://www.mdpi.com/2079-4991/11/5/1152hydroxyapatitenanoparticlescytotoxicitygenotoxicitymicronucleus testcellular uptake |
| spellingShingle | Rafaela-Maria Kavasi Catarina C. Coelho Varvara Platania Paulo A. Quadros Maria Chatzinikolaidou In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite Nanomaterials hydroxyapatite nanoparticles cytotoxicity genotoxicity micronucleus test cellular uptake |
| title | In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite |
| title_full | In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite |
| title_fullStr | In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite |
| title_full_unstemmed | In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite |
| title_short | In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite |
| title_sort | in vitro biocompatibility assessment of nano hydroxyapatite |
| topic | hydroxyapatite nanoparticles cytotoxicity genotoxicity micronucleus test cellular uptake |
| url | https://www.mdpi.com/2079-4991/11/5/1152 |
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