Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties
Background: N-acylated homoserine lactone lactonase which cleave the Acyl homoserine lactone molecules produced by biofilm-forming pathogens and silver nano-particles (AgNPs), are known for their antibacterial effect against several Gram-positive and Gram-negative bacteria. In this study, AgNPs were...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmolb.2019.00063/full |
| _version_ | 1819264804733321216 |
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| author | Kshitiz Gupta Sanjay Chhibber |
| author_facet | Kshitiz Gupta Sanjay Chhibber |
| author_sort | Kshitiz Gupta |
| collection | DOAJ |
| description | Background: N-acylated homoserine lactone lactonase which cleave the Acyl homoserine lactone molecules produced by biofilm-forming pathogens and silver nano-particles (AgNPs), are known for their antibacterial effect against several Gram-positive and Gram-negative bacteria. In this study, AgNPs were coated with N-acylated homoserine lactonase protein (AgNPs-AiiA) isolated from Bacillus sp. ZA12.Results: The AgNPs-AiiA complex was characterized by UV-visible spectra, Dynamic light Scattering, Fourier transform infrared spectroscopy (FTIR), and Field Emission Scanning Electron Microscope (Fe-SEM). The synthesized nano-particles were found to be spherical in shape and had an approximate size of 22.4 nm. Treatment with AiiA coated AgNPs showed a significant reduction in exopolysaccharide production, metabolic activity, cell surface hydrophobicity of bacterial cells, and anti-biofilm activity against multidrug-resistant K. pneumoniae as compared to treatment with AiiA protein and neat AgNPs. AgNPs-AiiA complex exhibited potent antibiofilm activity at sub-optimal concentration of 14.4 μg/mL without being harmful to the macrophages and to the various tissues including kidney, liver, spleen and lungs of BALB/c mice upon intra-venous administration.Conclusion: It is concluded that at a concentration of 14.4 μg/mL, AgNPs coated with AiiA kill bacteria without harming the host tissue and provides a suitable template to design novel anti-biofilm drug to circumvent the issue of drug resistance. |
| first_indexed | 2024-12-23T20:35:18Z |
| format | Article |
| id | doaj.art-708f57276a7a44658a28cdd48e6e0a85 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-23T20:35:18Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-708f57276a7a44658a28cdd48e6e0a852022-12-21T17:32:07ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2019-08-01610.3389/fmolb.2019.00063450649Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic PropertiesKshitiz GuptaSanjay ChhibberBackground: N-acylated homoserine lactone lactonase which cleave the Acyl homoserine lactone molecules produced by biofilm-forming pathogens and silver nano-particles (AgNPs), are known for their antibacterial effect against several Gram-positive and Gram-negative bacteria. In this study, AgNPs were coated with N-acylated homoserine lactonase protein (AgNPs-AiiA) isolated from Bacillus sp. ZA12.Results: The AgNPs-AiiA complex was characterized by UV-visible spectra, Dynamic light Scattering, Fourier transform infrared spectroscopy (FTIR), and Field Emission Scanning Electron Microscope (Fe-SEM). The synthesized nano-particles were found to be spherical in shape and had an approximate size of 22.4 nm. Treatment with AiiA coated AgNPs showed a significant reduction in exopolysaccharide production, metabolic activity, cell surface hydrophobicity of bacterial cells, and anti-biofilm activity against multidrug-resistant K. pneumoniae as compared to treatment with AiiA protein and neat AgNPs. AgNPs-AiiA complex exhibited potent antibiofilm activity at sub-optimal concentration of 14.4 μg/mL without being harmful to the macrophages and to the various tissues including kidney, liver, spleen and lungs of BALB/c mice upon intra-venous administration.Conclusion: It is concluded that at a concentration of 14.4 μg/mL, AgNPs coated with AiiA kill bacteria without harming the host tissue and provides a suitable template to design novel anti-biofilm drug to circumvent the issue of drug resistance.https://www.frontiersin.org/article/10.3389/fmolb.2019.00063/fullsilver nanoparticleslactonaseantibiofilm activitycell cytotoxicityantivirulence |
| spellingShingle | Kshitiz Gupta Sanjay Chhibber Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties Frontiers in Molecular Biosciences silver nanoparticles lactonase antibiofilm activity cell cytotoxicity antivirulence |
| title | Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties |
| title_full | Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties |
| title_fullStr | Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties |
| title_full_unstemmed | Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties |
| title_short | Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties |
| title_sort | biofunctionalization of silver nanoparticles with lactonase leads to altered antimicrobial and cytotoxic properties |
| topic | silver nanoparticles lactonase antibiofilm activity cell cytotoxicity antivirulence |
| url | https://www.frontiersin.org/article/10.3389/fmolb.2019.00063/full |
| work_keys_str_mv | AT kshitizgupta biofunctionalizationofsilvernanoparticleswithlactonaseleadstoalteredantimicrobialandcytotoxicproperties AT sanjaychhibber biofunctionalizationofsilvernanoparticleswithlactonaseleadstoalteredantimicrobialandcytotoxicproperties |