Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization
Abstract Somatic cell nuclear transfer technique (SCNT) has proved to be an outstanding method of multiplication of elite animals but accompanied with low efficiency and live birth rate of cloned animals. Epigenetic alterations of DNA has been one of the culprits behind this issue. Cloned embryos ar...
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| Format: | Article |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09207-8 |
| _version_ | 1811335437338279936 |
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| author | Shivani Malpotra Pallavi Goel Songyukta Shyam Manoj Kumar Singh Prabhat Palta |
| author_facet | Shivani Malpotra Pallavi Goel Songyukta Shyam Manoj Kumar Singh Prabhat Palta |
| author_sort | Shivani Malpotra |
| collection | DOAJ |
| description | Abstract Somatic cell nuclear transfer technique (SCNT) has proved to be an outstanding method of multiplication of elite animals but accompanied with low efficiency and live birth rate of cloned animals. Epigenetic alterations of DNA has been one of the culprits behind this issue. Cloned embryos are found to deviate slightly from regular pattern of demethylation and re-methylation at the time of nuclear reprogramming and embryonic development when compared with embryos produced by in vitro fertilization (IVF). Thus, the present study was aimed at evaluating global DNA methylation profiles of cloned embryos at 2-cell, 8-cell and blastocyst stages and compare it with corresponding stages of embryos produced by IVF by using MeDIP-Sequencing on Illumina-based platform. We found out that cloned embryos exhibited significantly different DNA methylation pattern as compared to IVF embryos with respect to distribution of differentially methylated regions in different components of genome, CpG islands distribution and methylation status, gene ontological profiles and pathways affected throughout the developmental stages. The data generated from MeDIP-Seq was validated at blastocyst stage cloned and IVF embryos by bisulfite-sequencing PCR on five randomly selected gene regions. |
| first_indexed | 2024-04-13T17:24:13Z |
| format | Article |
| id | doaj.art-70902dd23a0442d387a2c71f58761f3e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T17:24:13Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-70902dd23a0442d387a2c71f58761f3e2022-12-22T02:37:51ZengNature PortfolioScientific Reports2045-23222022-03-0112111810.1038/s41598-022-09207-8Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilizationShivani Malpotra0Pallavi Goel1Songyukta Shyam2Manoj Kumar Singh3Prabhat Palta4Embryo Biotechnology Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (Deemed University)Embryo Biotechnology Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (Deemed University)Embryo Biotechnology Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (Deemed University)Embryo Biotechnology Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (Deemed University)Embryo Biotechnology Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (Deemed University)Abstract Somatic cell nuclear transfer technique (SCNT) has proved to be an outstanding method of multiplication of elite animals but accompanied with low efficiency and live birth rate of cloned animals. Epigenetic alterations of DNA has been one of the culprits behind this issue. Cloned embryos are found to deviate slightly from regular pattern of demethylation and re-methylation at the time of nuclear reprogramming and embryonic development when compared with embryos produced by in vitro fertilization (IVF). Thus, the present study was aimed at evaluating global DNA methylation profiles of cloned embryos at 2-cell, 8-cell and blastocyst stages and compare it with corresponding stages of embryos produced by IVF by using MeDIP-Sequencing on Illumina-based platform. We found out that cloned embryos exhibited significantly different DNA methylation pattern as compared to IVF embryos with respect to distribution of differentially methylated regions in different components of genome, CpG islands distribution and methylation status, gene ontological profiles and pathways affected throughout the developmental stages. The data generated from MeDIP-Seq was validated at blastocyst stage cloned and IVF embryos by bisulfite-sequencing PCR on five randomly selected gene regions.https://doi.org/10.1038/s41598-022-09207-8 |
| spellingShingle | Shivani Malpotra Pallavi Goel Songyukta Shyam Manoj Kumar Singh Prabhat Palta Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization Scientific Reports |
| title | Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization |
| title_full | Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization |
| title_fullStr | Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization |
| title_full_unstemmed | Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization |
| title_short | Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization |
| title_sort | global dna methylation profiles of buffalo bubalus bubalis preimplantation embryos produced by handmade cloning and in vitro fertilization |
| url | https://doi.org/10.1038/s41598-022-09207-8 |
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