Wild Italian <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> (Godr.) Nyman: From Morphological and Phytochemical Evidences to Biological Activities
Three specimens of <i>H. officinalis</i> subsp. <i>aristatus</i> were collected in three areas of the Abruzzo region (Italy) and subjected to macroscopic and microscopic observation to support their botanical identification. The essential oils (EOs) obtained from the aerial p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/4/631 |
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| author | Alessandra Guerrini Gianni Sacchetti Monica Paulina Echeverria Guevara Guglielmo Paganetto Alessandro Grandini Immacolata Maresca Luigi Menghini Luciano Di Martino Arianna Marengo Massimo Tacchini |
| author_facet | Alessandra Guerrini Gianni Sacchetti Monica Paulina Echeverria Guevara Guglielmo Paganetto Alessandro Grandini Immacolata Maresca Luigi Menghini Luciano Di Martino Arianna Marengo Massimo Tacchini |
| author_sort | Alessandra Guerrini |
| collection | DOAJ |
| description | Three specimens of <i>H. officinalis</i> subsp. <i>aristatus</i> were collected in three areas of the Abruzzo region (Italy) and subjected to macroscopic and microscopic observation to support their botanical identification. The essential oils (EOs) obtained from the aerial parts of the samples were characterized with the object to define their phytochemical and pharmaceutical biology profile. They highlight three different chemotypes, including one never seen in previous literature (CIV17-EO, distilled from sample harvested in 2017 at Civitaretenga), that showed a fingerprinting with the predominance of (-)-limonen-10-yl-acetate (67.9%). In 2017 European Food Safety Authority (EFSA) reported the genotoxicity of similar compounds, therefore, to dismiss any safety concern for the CIV17-EO use as flavouring substance, the Ames test was performed with no evidence of mutagenic activity. Safety of use coupled with chemical characterization of this new chemotype set the stage for a better standardization of <i>H. officinalis</i> EOs. The ethanolic extracts, on the other hand, with qualitatively similar chemical profiles in which caftaric, chlorogenic and rosmarinic acid were the main molecules, showed interesting antioxidant activity and a slight cytotoxicity towards the A549 cell line that could indicate a starting point for the evaluation of an additional preventive tool for maintaining health status. |
| first_indexed | 2024-03-10T12:52:17Z |
| format | Article |
| id | doaj.art-709166c1fdab4c05be522cbf85a622e6 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T12:52:17Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-709166c1fdab4c05be522cbf85a622e62023-11-21T12:59:02ZengMDPI AGPlants2223-77472021-03-0110463110.3390/plants10040631Wild Italian <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> (Godr.) Nyman: From Morphological and Phytochemical Evidences to Biological ActivitiesAlessandra Guerrini0Gianni Sacchetti1Monica Paulina Echeverria Guevara2Guglielmo Paganetto3Alessandro Grandini4Immacolata Maresca5Luigi Menghini6Luciano Di Martino7Arianna Marengo8Massimo Tacchini9Pharmaceutical Biology Lab., Research Unit 7 of Terra&Acqua Tech Technopole Lab., Department of Life Sciences and Biotechnology, University of Ferrara, Piazzale Luciano Chiappini, 3, 44123 Ferrara, ItalyPharmaceutical Biology Lab., Research Unit 7 of Terra&Acqua Tech Technopole Lab., Department of Life Sciences and Biotechnology, University of Ferrara, Piazzale Luciano Chiappini, 3, 44123 Ferrara, ItalyDepartment of Earth Science, Universidad Estatal Amazónica, Puyo 160106, EcuadorPharmaceutical Biology Lab., Research Unit 7 of Terra&Acqua Tech Technopole Lab., Department of Life Sciences and Biotechnology, University of Ferrara, Piazzale Luciano Chiappini, 3, 44123 Ferrara, ItalyPharmaceutical Biology Lab., Research Unit 7 of Terra&Acqua Tech Technopole Lab., Department of Life Sciences and Biotechnology, University of Ferrara, Piazzale Luciano Chiappini, 3, 44123 Ferrara, ItalyPharmaceutical Biology Lab., Research Unit 7 of Terra&Acqua Tech Technopole Lab., Department of Life Sciences and Biotechnology, University of Ferrara, Piazzale Luciano Chiappini, 3, 44123 Ferrara, ItalyDepartment of Pharmacy, Botanical Garden “Giardino dei Semplici”, Università degli Studi “Gabriele d’Annunzio”, Via dei Vestini 31, 66100 Chieti, ItalyUfficio Monitoraggio e Conservazione Biodiversità Vegetale, Ente Parco Nazionale della Majella, Via Badia, 28 loc. Badia Morronese, 67039 Sulmona (AQ), ItalyDepartment of Drug Science and Technology, University of Torino, Via P. Giuria 9, 10125 Torino, ItalyPharmaceutical Biology Lab., Research Unit 7 of Terra&Acqua Tech Technopole Lab., Department of Life Sciences and Biotechnology, University of Ferrara, Piazzale Luciano Chiappini, 3, 44123 Ferrara, ItalyThree specimens of <i>H. officinalis</i> subsp. <i>aristatus</i> were collected in three areas of the Abruzzo region (Italy) and subjected to macroscopic and microscopic observation to support their botanical identification. The essential oils (EOs) obtained from the aerial parts of the samples were characterized with the object to define their phytochemical and pharmaceutical biology profile. They highlight three different chemotypes, including one never seen in previous literature (CIV17-EO, distilled from sample harvested in 2017 at Civitaretenga), that showed a fingerprinting with the predominance of (-)-limonen-10-yl-acetate (67.9%). In 2017 European Food Safety Authority (EFSA) reported the genotoxicity of similar compounds, therefore, to dismiss any safety concern for the CIV17-EO use as flavouring substance, the Ames test was performed with no evidence of mutagenic activity. Safety of use coupled with chemical characterization of this new chemotype set the stage for a better standardization of <i>H. officinalis</i> EOs. The ethanolic extracts, on the other hand, with qualitatively similar chemical profiles in which caftaric, chlorogenic and rosmarinic acid were the main molecules, showed interesting antioxidant activity and a slight cytotoxicity towards the A549 cell line that could indicate a starting point for the evaluation of an additional preventive tool for maintaining health status.https://www.mdpi.com/2223-7747/10/4/631<i>Hyssopus officinalis</i> subsp. <i>aristatus</i>macro and microscopic analysisessential oilsethanolic extractchemical characterizationantioxidant |
| spellingShingle | Alessandra Guerrini Gianni Sacchetti Monica Paulina Echeverria Guevara Guglielmo Paganetto Alessandro Grandini Immacolata Maresca Luigi Menghini Luciano Di Martino Arianna Marengo Massimo Tacchini Wild Italian <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> (Godr.) Nyman: From Morphological and Phytochemical Evidences to Biological Activities Plants <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> macro and microscopic analysis essential oils ethanolic extract chemical characterization antioxidant |
| title | Wild Italian <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> (Godr.) Nyman: From Morphological and Phytochemical Evidences to Biological Activities |
| title_full | Wild Italian <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> (Godr.) Nyman: From Morphological and Phytochemical Evidences to Biological Activities |
| title_fullStr | Wild Italian <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> (Godr.) Nyman: From Morphological and Phytochemical Evidences to Biological Activities |
| title_full_unstemmed | Wild Italian <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> (Godr.) Nyman: From Morphological and Phytochemical Evidences to Biological Activities |
| title_short | Wild Italian <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> (Godr.) Nyman: From Morphological and Phytochemical Evidences to Biological Activities |
| title_sort | wild italian i hyssopus officinalis i subsp i aristatus i godr nyman from morphological and phytochemical evidences to biological activities |
| topic | <i>Hyssopus officinalis</i> subsp. <i>aristatus</i> macro and microscopic analysis essential oils ethanolic extract chemical characterization antioxidant |
| url | https://www.mdpi.com/2223-7747/10/4/631 |
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