Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (<i>Triticum aestivum</i> L.)
Lab-on-a-chip technology is an emerging and convenient system to easily and quickly separate proteins of high molecular weight. The current study established a high-molecular-weight glutenin subunit (HMW-GS) identification system using Lab-on-a-chip for three, six, and three of the allelic variation...
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MDPI AG
2020-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/11/1517 |
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| author | Dongjin Shin Jin-Kyung Cha So-Myeong Lee Nkulu Rolly Kabange Jong-Hee Lee |
| author_facet | Dongjin Shin Jin-Kyung Cha So-Myeong Lee Nkulu Rolly Kabange Jong-Hee Lee |
| author_sort | Dongjin Shin |
| collection | DOAJ |
| description | Lab-on-a-chip technology is an emerging and convenient system to easily and quickly separate proteins of high molecular weight. The current study established a high-molecular-weight glutenin subunit (HMW-GS) identification system using Lab-on-a-chip for three, six, and three of the allelic variations at the <i>Glu-A1</i>, <i>Glu-B1</i>, and <i>Glu-D1</i> loci, respectively, which are commonly used in wheat breeding programs. The molecular weight of 1Ax1 and 1Ax2* encoded by <i>Glu-A1</i> locus were of 200 kDa and 192 kDa and positioned below 1Dx subunits. The HMW-GS encoded by <i>Glu-B1</i> locus were electrophoresed in the following order below 1Ax1 and 1Ax2*: 1Bx13 ≥ 1Bx7 = 1Bx7<sup>OE</sup> > 1Bx17 > 1By16 > 1By8 = 1By18 > 1By9. 1Dx2 and Dx5 showed around 4-kDa difference in their molecular weights, with 1Dy10 and 1Dy12 having 11-kDa difference, and were clearly differentiated on Lab-on-a-chip. Additionally, some of the HMW-GS, including 1By8, 1By18, and 1Dy10, having different theoretical molecular weights showed similar electrophoretic mobility patterns on Lab-on-a-chip. The relative protein amount of 1Bx7<sup>OE</sup> was two-fold higher than that of 1Bx7 or 1Dx5 and, therefore, translated a significant increase in the protein amount in 1Bx7<sup>OE</sup>. Similarly, the relative protein amounts of 8 & 10 and 10 & 18 were higher than each subunit taken alone. Therefore, this study suggests the established HMW-GS identification system using Lab-on-a-chip as a reliable approach for evaluating HMW-GS for wheat breeding programs. |
| first_indexed | 2024-03-10T14:59:50Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:59:50Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-7091a8df89484e1e90be720cd316412c2023-11-20T20:15:33ZengMDPI AGPlants2223-77472020-11-01911151710.3390/plants9111517Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (<i>Triticum aestivum</i> L.)Dongjin Shin0Jin-Kyung Cha1So-Myeong Lee2Nkulu Rolly Kabange3Jong-Hee Lee4Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, KoreaLab-on-a-chip technology is an emerging and convenient system to easily and quickly separate proteins of high molecular weight. The current study established a high-molecular-weight glutenin subunit (HMW-GS) identification system using Lab-on-a-chip for three, six, and three of the allelic variations at the <i>Glu-A1</i>, <i>Glu-B1</i>, and <i>Glu-D1</i> loci, respectively, which are commonly used in wheat breeding programs. The molecular weight of 1Ax1 and 1Ax2* encoded by <i>Glu-A1</i> locus were of 200 kDa and 192 kDa and positioned below 1Dx subunits. The HMW-GS encoded by <i>Glu-B1</i> locus were electrophoresed in the following order below 1Ax1 and 1Ax2*: 1Bx13 ≥ 1Bx7 = 1Bx7<sup>OE</sup> > 1Bx17 > 1By16 > 1By8 = 1By18 > 1By9. 1Dx2 and Dx5 showed around 4-kDa difference in their molecular weights, with 1Dy10 and 1Dy12 having 11-kDa difference, and were clearly differentiated on Lab-on-a-chip. Additionally, some of the HMW-GS, including 1By8, 1By18, and 1Dy10, having different theoretical molecular weights showed similar electrophoretic mobility patterns on Lab-on-a-chip. The relative protein amount of 1Bx7<sup>OE</sup> was two-fold higher than that of 1Bx7 or 1Dx5 and, therefore, translated a significant increase in the protein amount in 1Bx7<sup>OE</sup>. Similarly, the relative protein amounts of 8 & 10 and 10 & 18 were higher than each subunit taken alone. Therefore, this study suggests the established HMW-GS identification system using Lab-on-a-chip as a reliable approach for evaluating HMW-GS for wheat breeding programs.https://www.mdpi.com/2223-7747/9/11/1517HMW-GSLab-on-a-chipelectropherogramwheat |
| spellingShingle | Dongjin Shin Jin-Kyung Cha So-Myeong Lee Nkulu Rolly Kabange Jong-Hee Lee Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (<i>Triticum aestivum</i> L.) Plants HMW-GS Lab-on-a-chip electropherogram wheat |
| title | Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (<i>Triticum aestivum</i> L.) |
| title_full | Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (<i>Triticum aestivum</i> L.) |
| title_fullStr | Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (<i>Triticum aestivum</i> L.) |
| title_full_unstemmed | Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (<i>Triticum aestivum</i> L.) |
| title_short | Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (<i>Triticum aestivum</i> L.) |
| title_sort | rapid and easy high molecular weight glutenin subunit identification system by lab on a chip in wheat i triticum aestivum i l |
| topic | HMW-GS Lab-on-a-chip electropherogram wheat |
| url | https://www.mdpi.com/2223-7747/9/11/1517 |
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