Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (<i>Brassica napus</i> L.)
Nitrogen remobilization processes from source to sink tissues in plants are determinant for seed yield and their implementation results in a complete reorganization of the primary metabolism during sink/source transition. Here, we decided to characterize the impact of the sink/source balance on amin...
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MDPI AG
2020-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/4/150 |
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| author | Younès Dellero Maud Heuillet Nathalie Marnet Floriant Bellvert Pierre Millard Alain Bouchereau |
| author_facet | Younès Dellero Maud Heuillet Nathalie Marnet Floriant Bellvert Pierre Millard Alain Bouchereau |
| author_sort | Younès Dellero |
| collection | DOAJ |
| description | Nitrogen remobilization processes from source to sink tissues in plants are determinant for seed yield and their implementation results in a complete reorganization of the primary metabolism during sink/source transition. Here, we decided to characterize the impact of the sink/source balance on amino acid metabolism in the leaves of winter oilseed rape grown at the vegetative stage. We combined a quantitative metabolomics approach with an instationary <sup>15</sup>N-labeling experiment by using [<sup>15</sup>N]<sub>L</sub>-glycine as a metabolic probe on leaf ranks with a gradual increase in their source status. We showed that the acquisition of the source status by leaves was specifically accompanied by a decrease in asparagine, glutamine, proline and S-methyl-l-cysteine sulphoxide contents and an increase in valine and threonine contents. Dynamic analysis of <sup>15</sup>N enrichment and concentration of amino acids revealed gradual changes in the dynamics of amino acid metabolism with respect to the sink/source status of leaf ranks. Notably, nitrogen assimilation into valine, threonine and proline were all decreased in source leaves compared to sink leaves. Overall, our results suggested a reduction in de novo amino acid biosynthesis during sink/source transition at the vegetative stage. |
| first_indexed | 2024-03-10T20:30:06Z |
| format | Article |
| id | doaj.art-7093d417473240b7bd8ab10351d78d40 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T20:30:06Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-7093d417473240b7bd8ab10351d78d402023-11-19T21:30:48ZengMDPI AGMetabolites2218-19892020-04-0110415010.3390/metabo10040150Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (<i>Brassica napus</i> L.)Younès Dellero0Maud Heuillet1Nathalie Marnet2Floriant Bellvert3Pierre Millard4Alain Bouchereau5Department Plant Biology and Breeding, Agrocampus Ouest, Institute for Genetics, Environment and Plant Protection, French National Research Institute for Agriculture, Food and Environment, University of Rennes II, 35653 Le Rheu, FranceDepartment Plant Biology and Breeding, Department Microbiology and Food Chain, INSA, TBI, French National Center for Scientific Research, French National Research Institute for Agriculture, Food and Environment, University of Toulouse, 31400 Toulouse, FranceDepartment Plant Biology and Breeding and Department Transform, Agrocampus Ouest, Plateau de Profilage Métabolique et Métabolique (P2M2), Biopolymers Interactions Assemblies, Institute for Genetics, Environment and Plant Protection, French National Research Institute for Agriculture, Food and Environment, University of Rennes II, 35653 Le Rheu, FranceDepartment Plant Biology and Breeding, Department Microbiology and Food Chain, INSA, TBI, French National Center for Scientific Research, French National Research Institute for Agriculture, Food and Environment, University of Toulouse, 31400 Toulouse, FranceDepartment Plant Biology and Breeding, Department Microbiology and Food Chain, INSA, TBI, French National Center for Scientific Research, French National Research Institute for Agriculture, Food and Environment, University of Toulouse, 31400 Toulouse, FranceDepartment Plant Biology and Breeding, Agrocampus Ouest, Institute for Genetics, Environment and Plant Protection, French National Research Institute for Agriculture, Food and Environment, University of Rennes II, 35653 Le Rheu, FranceNitrogen remobilization processes from source to sink tissues in plants are determinant for seed yield and their implementation results in a complete reorganization of the primary metabolism during sink/source transition. Here, we decided to characterize the impact of the sink/source balance on amino acid metabolism in the leaves of winter oilseed rape grown at the vegetative stage. We combined a quantitative metabolomics approach with an instationary <sup>15</sup>N-labeling experiment by using [<sup>15</sup>N]<sub>L</sub>-glycine as a metabolic probe on leaf ranks with a gradual increase in their source status. We showed that the acquisition of the source status by leaves was specifically accompanied by a decrease in asparagine, glutamine, proline and S-methyl-l-cysteine sulphoxide contents and an increase in valine and threonine contents. Dynamic analysis of <sup>15</sup>N enrichment and concentration of amino acids revealed gradual changes in the dynamics of amino acid metabolism with respect to the sink/source status of leaf ranks. Notably, nitrogen assimilation into valine, threonine and proline were all decreased in source leaves compared to sink leaves. Overall, our results suggested a reduction in de novo amino acid biosynthesis during sink/source transition at the vegetative stage.https://www.mdpi.com/2218-1989/10/4/150metabolomicsisotopically non-stationary steady-state metabolic flux analysis<i>Brassica napus</i>winter oilseed rapesink/source balancesenescence |
| spellingShingle | Younès Dellero Maud Heuillet Nathalie Marnet Floriant Bellvert Pierre Millard Alain Bouchereau Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (<i>Brassica napus</i> L.) Metabolites metabolomics isotopically non-stationary steady-state metabolic flux analysis <i>Brassica napus</i> winter oilseed rape sink/source balance senescence |
| title | Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (<i>Brassica napus</i> L.) |
| title_full | Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (<i>Brassica napus</i> L.) |
| title_fullStr | Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (<i>Brassica napus</i> L.) |
| title_full_unstemmed | Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (<i>Brassica napus</i> L.) |
| title_short | Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (<i>Brassica napus</i> L.) |
| title_sort | sink source balance of leaves influences amino acid pools and their associated metabolic fluxes in winter oilseed rape i brassica napus i l |
| topic | metabolomics isotopically non-stationary steady-state metabolic flux analysis <i>Brassica napus</i> winter oilseed rape sink/source balance senescence |
| url | https://www.mdpi.com/2218-1989/10/4/150 |
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