Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2014-12-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057&lng=en&tlng=en |
| _version_ | 1818065602830401536 |
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| author | GRASIELA DEMARCHI VALDIR M. STEFENON NEUSA STEINER FELIPE N. VIEIRA LIRIO L. DAL VESCO MIGUEL P. GUERRA |
| author_facet | GRASIELA DEMARCHI VALDIR M. STEFENON NEUSA STEINER FELIPE N. VIEIRA LIRIO L. DAL VESCO MIGUEL P. GUERRA |
| author_sort | GRASIELA DEMARCHI |
| collection | DOAJ |
| description | This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic. |
| first_indexed | 2024-12-10T14:54:30Z |
| format | Article |
| id | doaj.art-7096367d8a4041a883a6e923a6cfa978 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-10T14:54:30Z |
| publishDate | 2014-12-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-7096367d8a4041a883a6e923a6cfa9782022-12-22T01:44:20ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902014-12-018642057206410.1590/0001-3765201420130405S0001-37652014000402057Ultra-low temperature conservation of Brazilian Pine embryogenic culturesGRASIELA DEMARCHIVALDIR M. STEFENONNEUSA STEINERFELIPE N. VIEIRALIRIO L. DAL VESCOMIGUEL P. GUERRAThis study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057&lng=en&tlng=encriopreservaçãoconservação ex situcultura in vitroconservação de germoplasma vegetal |
| spellingShingle | GRASIELA DEMARCHI VALDIR M. STEFENON NEUSA STEINER FELIPE N. VIEIRA LIRIO L. DAL VESCO MIGUEL P. GUERRA Ultra-low temperature conservation of Brazilian Pine embryogenic cultures Anais da Academia Brasileira de Ciências criopreservação conservação ex situ cultura in vitro conservação de germoplasma vegetal |
| title | Ultra-low temperature conservation of Brazilian Pine embryogenic cultures |
| title_full | Ultra-low temperature conservation of Brazilian Pine embryogenic cultures |
| title_fullStr | Ultra-low temperature conservation of Brazilian Pine embryogenic cultures |
| title_full_unstemmed | Ultra-low temperature conservation of Brazilian Pine embryogenic cultures |
| title_short | Ultra-low temperature conservation of Brazilian Pine embryogenic cultures |
| title_sort | ultra low temperature conservation of brazilian pine embryogenic cultures |
| topic | criopreservação conservação ex situ cultura in vitro conservação de germoplasma vegetal |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057&lng=en&tlng=en |
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