Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses
Osteoarthritis (OA) is a common cause of lameness in sport horses with a significant economic impact. The prevention of OA is crucial since no effective treatment is available. This study aimed to apply untargeted metabolomic analysis to investigate the differences in synovial fluid (SF) composition...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/8/913 |
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| author | Fulvio Laus Rodolfo Gialletti Marilena Bazzano Luca Laghi Fabrizio Dini Andrea Marchegiani |
| author_facet | Fulvio Laus Rodolfo Gialletti Marilena Bazzano Luca Laghi Fabrizio Dini Andrea Marchegiani |
| author_sort | Fulvio Laus |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a common cause of lameness in sport horses with a significant economic impact. The prevention of OA is crucial since no effective treatment is available. This study aimed to apply untargeted metabolomic analysis to investigate the differences in synovial fluid (SF) composition between healthy and OA-affected joints in horses. SF collected from healthy (n.8) and OA (n.11) horses was analyzed using H-NMR analysis. Metabolomic analysis allowed 55 different metabolites to be identified and quantified in SF samples. Nineteen metabolites were found to be differently concentrated in OA compared to control horses. Synovial fluids from the OC group were found to be higher in 1,3-dihydroxyacetone but lower in tryptophan, phenylalanine, tyrosine, uridine, creatinine, creatine, glycine, choline, asparagine, glutamine, arginine, 3-hydroxybutyrate, valine, 2-hydroxyisovalerate, α-ketoisovaleric acid, 3-methyl-2-oxovalerate, 3-hydroxyisobutyrate, isoleucine, and methionine compared to the controls. A variety of SF metabolites significantly changed following joint disease, demonstrating the complex mechanism underlying osteoarthritis in horses and highlighting the value of applying the metabolomic approach in clinical research. |
| first_indexed | 2024-03-10T23:44:38Z |
| format | Article |
| id | doaj.art-70965a7b95af49eba98bd5f2c990adc0 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T23:44:38Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-70965a7b95af49eba98bd5f2c990adc02023-11-19T02:08:46ZengMDPI AGMetabolites2218-19892023-08-0113891310.3390/metabo13080913Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in HorsesFulvio Laus0Rodolfo Gialletti1Marilena Bazzano2Luca Laghi3Fabrizio Dini4Andrea Marchegiani5School of Biosciences and Veterinary Medicine, University of Camerino, 62024 Macerata, ItalyDepartment of Veterinary Medicine, University of Perugia, 06123 Perugia, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62024 Macerata, ItalyCentre of Foodomics, Department of Agro-Food Science and Technology, University of Bologna, 40100 Bologna, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62024 Macerata, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62024 Macerata, ItalyOsteoarthritis (OA) is a common cause of lameness in sport horses with a significant economic impact. The prevention of OA is crucial since no effective treatment is available. This study aimed to apply untargeted metabolomic analysis to investigate the differences in synovial fluid (SF) composition between healthy and OA-affected joints in horses. SF collected from healthy (n.8) and OA (n.11) horses was analyzed using H-NMR analysis. Metabolomic analysis allowed 55 different metabolites to be identified and quantified in SF samples. Nineteen metabolites were found to be differently concentrated in OA compared to control horses. Synovial fluids from the OC group were found to be higher in 1,3-dihydroxyacetone but lower in tryptophan, phenylalanine, tyrosine, uridine, creatinine, creatine, glycine, choline, asparagine, glutamine, arginine, 3-hydroxybutyrate, valine, 2-hydroxyisovalerate, α-ketoisovaleric acid, 3-methyl-2-oxovalerate, 3-hydroxyisobutyrate, isoleucine, and methionine compared to the controls. A variety of SF metabolites significantly changed following joint disease, demonstrating the complex mechanism underlying osteoarthritis in horses and highlighting the value of applying the metabolomic approach in clinical research.https://www.mdpi.com/2218-1989/13/8/913osteoarthritismetabolomeH-NMRhorse |
| spellingShingle | Fulvio Laus Rodolfo Gialletti Marilena Bazzano Luca Laghi Fabrizio Dini Andrea Marchegiani Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses Metabolites osteoarthritis metabolome H-NMR horse |
| title | Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses |
| title_full | Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses |
| title_fullStr | Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses |
| title_full_unstemmed | Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses |
| title_short | Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses |
| title_sort | synovial fluid metabolome can differentiate between healthy joints and joints affected by osteoarthritis in horses |
| topic | osteoarthritis metabolome H-NMR horse |
| url | https://www.mdpi.com/2218-1989/13/8/913 |
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