Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva.
The periodontium undergoes age-related cellular and clinical changes, but the involved genes are not yet known. Here, we investigated age-related genetic changes in gingiva at the transcriptomic level. Genes that were differentially expressed between young and old human gingiva were identified by RN...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4938517?pdf=render |
| _version_ | 1811209489051811840 |
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| author | Suhee Kim Sun Hee Ahn Jin-Sil Lee Ji-Eun Song Sung-Hyun Cho Seunggon Jung Seon-Kyu Kim Seok-Ho Kim Kwang-Pyo Lee Ki-Sun Kwon Tae-Hoon Lee |
| author_facet | Suhee Kim Sun Hee Ahn Jin-Sil Lee Ji-Eun Song Sung-Hyun Cho Seunggon Jung Seon-Kyu Kim Seok-Ho Kim Kwang-Pyo Lee Ki-Sun Kwon Tae-Hoon Lee |
| author_sort | Suhee Kim |
| collection | DOAJ |
| description | The periodontium undergoes age-related cellular and clinical changes, but the involved genes are not yet known. Here, we investigated age-related genetic changes in gingiva at the transcriptomic level. Genes that were differentially expressed between young and old human gingiva were identified by RNA sequencing and verified by real-time PCR. A total of 1939 mRNA transcripts showed significantly differential expression between young and old gingival tissues. Matrix metalloprotease (MMP) regulation was the top pathway involved in gingival aging. MMP3, MMP9, MMP12, and MMP13 were upregulated in old gingival tissues, concomitantly with interleukin-1 beta (IL1B) expression. In vitro experiments using human gingival fibroblasts (hGFs) showed that MMP12 was upregulated in old hGFs compared to young hGFs. Moreover, the MMP3, MMP9 and IL1B levels were more highly stimulated by infection with the oral bacterium, Fusobacterium nucleatum, in old hGFs compared to young hGFs. Collectively, these findings suggest that, in gingiva, the upregulation of MMP12 may be a molecular hallmark of natural aging, while the upregulations of MMP3, MMM9, and IL1B may indicate externally (e.g., infection)-induced aging. These findings contribute to our understanding of the molecular targets involved in gingival aging. |
| first_indexed | 2024-04-12T04:39:01Z |
| format | Article |
| id | doaj.art-709674407b0e425fa2dcbf604ec6a13a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:39:01Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-709674407b0e425fa2dcbf604ec6a13a2022-12-22T03:47:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e015877710.1371/journal.pone.0158777Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva.Suhee KimSun Hee AhnJin-Sil LeeJi-Eun SongSung-Hyun ChoSeunggon JungSeon-Kyu KimSeok-Ho KimKwang-Pyo LeeKi-Sun KwonTae-Hoon LeeThe periodontium undergoes age-related cellular and clinical changes, but the involved genes are not yet known. Here, we investigated age-related genetic changes in gingiva at the transcriptomic level. Genes that were differentially expressed between young and old human gingiva were identified by RNA sequencing and verified by real-time PCR. A total of 1939 mRNA transcripts showed significantly differential expression between young and old gingival tissues. Matrix metalloprotease (MMP) regulation was the top pathway involved in gingival aging. MMP3, MMP9, MMP12, and MMP13 were upregulated in old gingival tissues, concomitantly with interleukin-1 beta (IL1B) expression. In vitro experiments using human gingival fibroblasts (hGFs) showed that MMP12 was upregulated in old hGFs compared to young hGFs. Moreover, the MMP3, MMP9 and IL1B levels were more highly stimulated by infection with the oral bacterium, Fusobacterium nucleatum, in old hGFs compared to young hGFs. Collectively, these findings suggest that, in gingiva, the upregulation of MMP12 may be a molecular hallmark of natural aging, while the upregulations of MMP3, MMM9, and IL1B may indicate externally (e.g., infection)-induced aging. These findings contribute to our understanding of the molecular targets involved in gingival aging.http://europepmc.org/articles/PMC4938517?pdf=render |
| spellingShingle | Suhee Kim Sun Hee Ahn Jin-Sil Lee Ji-Eun Song Sung-Hyun Cho Seunggon Jung Seon-Kyu Kim Seok-Ho Kim Kwang-Pyo Lee Ki-Sun Kwon Tae-Hoon Lee Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva. PLoS ONE |
| title | Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva. |
| title_full | Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva. |
| title_fullStr | Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva. |
| title_full_unstemmed | Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva. |
| title_short | Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva. |
| title_sort | differential matrix metalloprotease mmp expression profiles found in aged gingiva |
| url | http://europepmc.org/articles/PMC4938517?pdf=render |
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