Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis
Navisha Dookie, A Willem Sturm, Prashini Moodley Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, Durban, South Africa Objectives: Moxifloxacin (MXF) has be...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2014-08-01
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| Series: | Infection and Drug Resistance |
| Online Access: | http://www.dovepress.com/moxifloxacin-resistance-in-the-f15lam4kzn-extensively-drug-resistant-s-peer-reviewed-article-IDR |
| _version_ | 1819124539972386816 |
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| author | Dookie N Sturm AW Moodley P |
| author_facet | Dookie N Sturm AW Moodley P |
| author_sort | Dookie N |
| collection | DOAJ |
| description | Navisha Dookie, A Willem Sturm, Prashini Moodley Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, Durban, South Africa Objectives: Moxifloxacin (MXF) has been advocated for the treatment of extensively drug-resistant (XDR) tuberculosis despite resistance to older-generation fluoroquinolones. We investigated the relationship between the minimum inhibitory concentration (MIC) of MXF and mutations in the gyrA and gyrB genes in Mycobacterium tuberculosis (MTB) isolates from KwaZulu-Natal (KZN) Province of South Africa. Materials and methods: MICs of 56 MTB isolates were compared to the mutations in the quinolone resistance-determining region known to confer fluoroquinolone resistance. Isolates were genotyped by IS6110 restriction fragment length polymorphism analysis. Results: The circulating F15/LAM4/KZN XDR strain circulating in KZN Province harbored the A90V mutation and displayed high-level resistance with MICs of 8 mg/L for ciprofloxacin and ofloxacin and ≥1 mg/L for MXF. Conclusion: The inclusion of MXF in XDR-TB treatment regimens requires careful consideration in our setting, where clinical outcome data in MXF-containing regimens are unavailable. Keywords: fluoroquinolones, susceptibility testing, strain typing, drug-resistance |
| first_indexed | 2024-12-22T07:25:52Z |
| format | Article |
| id | doaj.art-709746152d794e79bce33a8f1a6af17d |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-22T07:25:52Z |
| publishDate | 2014-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-709746152d794e79bce33a8f1a6af17d2022-12-21T18:34:08ZengDove Medical PressInfection and Drug Resistance1178-69732014-08-012014default22322817998Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosisDookie NSturm AWMoodley PNavisha Dookie, A Willem Sturm, Prashini Moodley Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, Durban, South Africa Objectives: Moxifloxacin (MXF) has been advocated for the treatment of extensively drug-resistant (XDR) tuberculosis despite resistance to older-generation fluoroquinolones. We investigated the relationship between the minimum inhibitory concentration (MIC) of MXF and mutations in the gyrA and gyrB genes in Mycobacterium tuberculosis (MTB) isolates from KwaZulu-Natal (KZN) Province of South Africa. Materials and methods: MICs of 56 MTB isolates were compared to the mutations in the quinolone resistance-determining region known to confer fluoroquinolone resistance. Isolates were genotyped by IS6110 restriction fragment length polymorphism analysis. Results: The circulating F15/LAM4/KZN XDR strain circulating in KZN Province harbored the A90V mutation and displayed high-level resistance with MICs of 8 mg/L for ciprofloxacin and ofloxacin and ≥1 mg/L for MXF. Conclusion: The inclusion of MXF in XDR-TB treatment regimens requires careful consideration in our setting, where clinical outcome data in MXF-containing regimens are unavailable. Keywords: fluoroquinolones, susceptibility testing, strain typing, drug-resistancehttp://www.dovepress.com/moxifloxacin-resistance-in-the-f15lam4kzn-extensively-drug-resistant-s-peer-reviewed-article-IDR |
| spellingShingle | Dookie N Sturm AW Moodley P Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis Infection and Drug Resistance |
| title | Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis |
| title_full | Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis |
| title_fullStr | Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis |
| title_full_unstemmed | Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis |
| title_short | Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis |
| title_sort | moxifloxacin resistance in the f15 lam4 kzn extensively drug resistant strain of mycobacterium tuberculosis |
| url | http://www.dovepress.com/moxifloxacin-resistance-in-the-f15lam4kzn-extensively-drug-resistant-s-peer-reviewed-article-IDR |
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