Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues
The production of lignocellulosic ethanol calls for a robust fermentative yeast able to tolerate a wide range of toxic molecules that occur in the pre-treated lignocellulose. The concentration of inhibitors varies according to the composition of the lignocellulosic material and the harshness of the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.756032/full |
| _version_ | 1830283730956058624 |
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| author | Lorenzo Cagnin Nicoletta Gronchi Marina Basaglia Lorenzo Favaro Sergio Casella |
| author_facet | Lorenzo Cagnin Nicoletta Gronchi Marina Basaglia Lorenzo Favaro Sergio Casella |
| author_sort | Lorenzo Cagnin |
| collection | DOAJ |
| description | The production of lignocellulosic ethanol calls for a robust fermentative yeast able to tolerate a wide range of toxic molecules that occur in the pre-treated lignocellulose. The concentration of inhibitors varies according to the composition of the lignocellulosic material and the harshness of the pre-treatment used. It follows that the versatility of the yeast should be considered when selecting a robust strain. This work aimed at the validation of seven natural Saccharomyces cerevisiae strains, previously selected for their industrial fitness, for their application in the production of lignocellulosic bioethanol. Their inhibitor resistance and fermentative performances were compared to those of the benchmark industrial yeast S. cerevisiae Ethanol Red, currently utilized in the second-generation ethanol plants. The yeast strains were characterized for their tolerance using a synthetic inhibitor mixture formulated with increasing concentrations of weak acids and furans, as well as steam-exploded lignocellulosic pre-hydrolysates, generally containing the same inhibitors. The eight non-diluted liquors have been adopted to assess yeast ability to withstand bioethanol industrial conditions. The most tolerant S. cerevisiae Fm17 strain, together with the reference Ethanol Red, was evaluated for fermentative performances in two pre-hydrolysates obtained from cardoon and common reed, chosen for their large inhibitor concentrations. S. cerevisiae Fm17 outperformed the industrial strain Ethanol Red, producing up to 18 and 39 g/L ethanol from cardoon and common reed, respectively, with ethanol yields always higher than those of the benchmark strain. This natural strain exhibits great potential to be used as superior yeast in the lignocellulosic ethanol plants. |
| first_indexed | 2024-12-19T03:05:18Z |
| format | Article |
| id | doaj.art-7099e36b9683457a954ce1c4eabf9e61 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T03:05:18Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7099e36b9683457a954ce1c4eabf9e612022-12-21T20:38:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.756032756032Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded ResiduesLorenzo CagninNicoletta GronchiMarina BasagliaLorenzo FavaroSergio CasellaThe production of lignocellulosic ethanol calls for a robust fermentative yeast able to tolerate a wide range of toxic molecules that occur in the pre-treated lignocellulose. The concentration of inhibitors varies according to the composition of the lignocellulosic material and the harshness of the pre-treatment used. It follows that the versatility of the yeast should be considered when selecting a robust strain. This work aimed at the validation of seven natural Saccharomyces cerevisiae strains, previously selected for their industrial fitness, for their application in the production of lignocellulosic bioethanol. Their inhibitor resistance and fermentative performances were compared to those of the benchmark industrial yeast S. cerevisiae Ethanol Red, currently utilized in the second-generation ethanol plants. The yeast strains were characterized for their tolerance using a synthetic inhibitor mixture formulated with increasing concentrations of weak acids and furans, as well as steam-exploded lignocellulosic pre-hydrolysates, generally containing the same inhibitors. The eight non-diluted liquors have been adopted to assess yeast ability to withstand bioethanol industrial conditions. The most tolerant S. cerevisiae Fm17 strain, together with the reference Ethanol Red, was evaluated for fermentative performances in two pre-hydrolysates obtained from cardoon and common reed, chosen for their large inhibitor concentrations. S. cerevisiae Fm17 outperformed the industrial strain Ethanol Red, producing up to 18 and 39 g/L ethanol from cardoon and common reed, respectively, with ethanol yields always higher than those of the benchmark strain. This natural strain exhibits great potential to be used as superior yeast in the lignocellulosic ethanol plants.https://www.frontiersin.org/articles/10.3389/fmicb.2021.756032/fullbioethanolsugarcane bagassecardooncommon reedindustrial yeast strainssteam explosion |
| spellingShingle | Lorenzo Cagnin Nicoletta Gronchi Marina Basaglia Lorenzo Favaro Sergio Casella Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues Frontiers in Microbiology bioethanol sugarcane bagasse cardoon common reed industrial yeast strains steam explosion |
| title | Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues |
| title_full | Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues |
| title_fullStr | Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues |
| title_full_unstemmed | Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues |
| title_short | Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues |
| title_sort | selection of superior yeast strains for the fermentation of lignocellulosic steam exploded residues |
| topic | bioethanol sugarcane bagasse cardoon common reed industrial yeast strains steam explosion |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.756032/full |
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