Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation
Abstract Lipoprotein monitoring is desirable in the management of medical conditions such as atherosclerotic cardiovascular disease and coronary artery disease, in which controlling the concentration of these chylomicrons is crucial. Current clinical methods are complex and present poor reproducibil...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2023-05-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100360&lng=en&tlng=en |
| _version_ | 1797826217414492160 |
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| author | Elton Max Nascimento do Egito Isaac Aaron Morales Frias Maria Danielly Lima Oliveira César Augusto Souza de Andrade |
| author_facet | Elton Max Nascimento do Egito Isaac Aaron Morales Frias Maria Danielly Lima Oliveira César Augusto Souza de Andrade |
| author_sort | Elton Max Nascimento do Egito |
| collection | DOAJ |
| description | Abstract Lipoprotein monitoring is desirable in the management of medical conditions such as atherosclerotic cardiovascular disease and coronary artery disease, in which controlling the concentration of these chylomicrons is crucial. Current clinical methods are complex and present poor reproducibility between laboratories. For these reasons, recent guidelines discard the assessment of low-density lipoprotein cholesterol (LDL-C) as a routine analysis during lipid-lowering therapies. Concerning the importance of monitoring this parameter, the authors present an electrochemical immunosensor constructed from a simple and easy-to-reproduce platform that allows detecting and quantifying LDL nanoparticles directly from human serum samples. The performance of the biosensor was studied by scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The biosensing platform displays good stability and linearity between 30 mg dL-1 and 135 mg dL-1 with a detection limit of 20 mg dL-1. The proposed biosensor can be easily employed for monitoring LDL concentration in clinical treatments. |
| first_indexed | 2024-03-13T11:05:29Z |
| format | Article |
| id | doaj.art-709db1b34dc64e7d992bf3bbd8ae655e |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-03-13T11:05:29Z |
| publishDate | 2023-05-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-709db1b34dc64e7d992bf3bbd8ae655e2023-05-16T07:33:49ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902023-05-015910.1590/s2175-97902023e22430Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulationElton Max Nascimento do EgitoIsaac Aaron Morales FriasMaria Danielly Lima OliveiraCésar Augusto Souza de Andradehttps://orcid.org/0000-0002-3271-2817Abstract Lipoprotein monitoring is desirable in the management of medical conditions such as atherosclerotic cardiovascular disease and coronary artery disease, in which controlling the concentration of these chylomicrons is crucial. Current clinical methods are complex and present poor reproducibility between laboratories. For these reasons, recent guidelines discard the assessment of low-density lipoprotein cholesterol (LDL-C) as a routine analysis during lipid-lowering therapies. Concerning the importance of monitoring this parameter, the authors present an electrochemical immunosensor constructed from a simple and easy-to-reproduce platform that allows detecting and quantifying LDL nanoparticles directly from human serum samples. The performance of the biosensor was studied by scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The biosensing platform displays good stability and linearity between 30 mg dL-1 and 135 mg dL-1 with a detection limit of 20 mg dL-1. The proposed biosensor can be easily employed for monitoring LDL concentration in clinical treatments.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100360&lng=en&tlng=enApolipoproteinBiosensorElectrochemistryHypercholesterolemiaSelf-assembled monolayerSol-gel |
| spellingShingle | Elton Max Nascimento do Egito Isaac Aaron Morales Frias Maria Danielly Lima Oliveira César Augusto Souza de Andrade Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation Brazilian Journal of Pharmaceutical Sciences Apolipoprotein Biosensor Electrochemistry Hypercholesterolemia Self-assembled monolayer Sol-gel |
| title | Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
| title_full | Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
| title_fullStr | Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
| title_full_unstemmed | Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
| title_short | Electrochemical immunosensing of low-density lipoprotein based on sol-gel encapsulation |
| title_sort | electrochemical immunosensing of low density lipoprotein based on sol gel encapsulation |
| topic | Apolipoprotein Biosensor Electrochemistry Hypercholesterolemia Self-assembled monolayer Sol-gel |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100360&lng=en&tlng=en |
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