Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma Cells
Enhancing nanoparticles’ anti-cancer capabilities as drug carriers requires the careful adjustment of formulation parameters, including loading efficiency, drug/carrier ratio, and synthesis method. Small adjustments to these parameters can significantly influence the drug-loading efficiency of nanop...
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MDPI AG
2024-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/15/2/43 |
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| author | Aynura Karimova Sabina Hajizada Habiba Shirinova Sevinj Nuriyeva Lala Gahramanli Mohammed M. Yusuf Stefano Bellucci Christoph Reissfelder Vugar Yagublu |
| author_facet | Aynura Karimova Sabina Hajizada Habiba Shirinova Sevinj Nuriyeva Lala Gahramanli Mohammed M. Yusuf Stefano Bellucci Christoph Reissfelder Vugar Yagublu |
| author_sort | Aynura Karimova |
| collection | DOAJ |
| description | Enhancing nanoparticles’ anti-cancer capabilities as drug carriers requires the careful adjustment of formulation parameters, including loading efficiency, drug/carrier ratio, and synthesis method. Small adjustments to these parameters can significantly influence the drug-loading efficiency of nanoparticles. Our study explored how chitosan and polyethylene glycol (PEG) coatings affect the structural properties, drug-loading efficiency, and anti-cancer efficacy of Fe<sub>3</sub>O<sub>4</sub> nanoparticles (NPs). The loading efficiency of the NPs was determined using FTIR spectrometry and XRD. The quantity of chrysin incorporated into the coated NPs was examined using UV–Vis spectrometry. The effect of the NPs on cell viability and apoptosis was determined by employing the HCT 116 human colon carcinoma cell line. We showed that a two-fold increase in drug concentration did not impact the loading efficiency of Fe<sub>3</sub>O<sub>4</sub> NPs coated with PEG. However, there was a 33 Å difference in the crystallite sizes obtained from chitosan-coated Fe<sub>3</sub>O<sub>4</sub> NPs and drug concentrations of 1:0.5 and 1:2, resulting in decreased system stability. In conclusion, PEG coating exhibited a higher loading efficiency of Fe<sub>3</sub>O<sub>4</sub> NPs compared to chitosan, resulting in enhanced anti-tumor effects. Furthermore, variations in the loaded amount of chrysin did not impact the crystallinity of PEG-coated NPs, emphasizing the stability and regularity of the system. |
| first_indexed | 2024-03-07T22:26:42Z |
| format | Article |
| id | doaj.art-709e8360416c45c2a9b07d88c28e90dd |
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| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-07T22:26:42Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-709e8360416c45c2a9b07d88c28e90dd2024-02-23T15:22:42ZengMDPI AGJournal of Functional Biomaterials2079-49832024-02-011524310.3390/jfb15020043Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma CellsAynura Karimova0Sabina Hajizada1Habiba Shirinova2Sevinj Nuriyeva3Lala Gahramanli4Mohammed M. Yusuf5Stefano Bellucci6Christoph Reissfelder7Vugar Yagublu8Nanoresearch Laboratory, Baku State University, Baku AZ 1148, AzerbaijanDepartment of Surgery, Medical Faculty Mannheim, University of Heidelberg, 68167 Mannheim, GermanyNanoresearch Laboratory, Baku State University, Baku AZ 1148, AzerbaijanNanoresearch Laboratory, Baku State University, Baku AZ 1148, AzerbaijanNanoresearch Laboratory, Baku State University, Baku AZ 1148, AzerbaijanDepartment of Surgery, Medical Faculty Mannheim, University of Heidelberg, 68167 Mannheim, GermanyLaboratori Nazionali di Frascati, Istituto Nazionale di Fisica Nucleare, 00044 Frascati, ItalyDepartment of Surgery, Medical Faculty Mannheim, University of Heidelberg, 68167 Mannheim, GermanyDepartment of Surgery, Medical Faculty Mannheim, University of Heidelberg, 68167 Mannheim, GermanyEnhancing nanoparticles’ anti-cancer capabilities as drug carriers requires the careful adjustment of formulation parameters, including loading efficiency, drug/carrier ratio, and synthesis method. Small adjustments to these parameters can significantly influence the drug-loading efficiency of nanoparticles. Our study explored how chitosan and polyethylene glycol (PEG) coatings affect the structural properties, drug-loading efficiency, and anti-cancer efficacy of Fe<sub>3</sub>O<sub>4</sub> nanoparticles (NPs). The loading efficiency of the NPs was determined using FTIR spectrometry and XRD. The quantity of chrysin incorporated into the coated NPs was examined using UV–Vis spectrometry. The effect of the NPs on cell viability and apoptosis was determined by employing the HCT 116 human colon carcinoma cell line. We showed that a two-fold increase in drug concentration did not impact the loading efficiency of Fe<sub>3</sub>O<sub>4</sub> NPs coated with PEG. However, there was a 33 Å difference in the crystallite sizes obtained from chitosan-coated Fe<sub>3</sub>O<sub>4</sub> NPs and drug concentrations of 1:0.5 and 1:2, resulting in decreased system stability. In conclusion, PEG coating exhibited a higher loading efficiency of Fe<sub>3</sub>O<sub>4</sub> NPs compared to chitosan, resulting in enhanced anti-tumor effects. Furthermore, variations in the loaded amount of chrysin did not impact the crystallinity of PEG-coated NPs, emphasizing the stability and regularity of the system.https://www.mdpi.com/2079-4983/15/2/43magnetite nanoparticlescoating agentsloading efficiencydrug concentrationcolon cancer |
| spellingShingle | Aynura Karimova Sabina Hajizada Habiba Shirinova Sevinj Nuriyeva Lala Gahramanli Mohammed M. Yusuf Stefano Bellucci Christoph Reissfelder Vugar Yagublu Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma Cells Journal of Functional Biomaterials magnetite nanoparticles coating agents loading efficiency drug concentration colon cancer |
| title | Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma Cells |
| title_full | Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma Cells |
| title_fullStr | Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma Cells |
| title_full_unstemmed | Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma Cells |
| title_short | Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma Cells |
| title_sort | surface modification strategies for chrysin loaded iron oxide nanoparticles to boost their anti tumor efficacy in human colon carcinoma cells |
| topic | magnetite nanoparticles coating agents loading efficiency drug concentration colon cancer |
| url | https://www.mdpi.com/2079-4983/15/2/43 |
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