Optimizing Growth Conditions of Kluyveromyces marxianus for Mannan Production as a Bioemulsifier
Background and objective: Mannan which is a linear glycoprotein with β-1,4 links carrying mannose units bind to proteins, includes natural amphiphiles and serves as a bioemulsifier. The aim of this study was optimization of growth and purification of Kluyveromyces marxianus for mannan production, wh...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Shahid Behehsti University of Medical Sciences
2020-03-01
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| Series: | Applied Food Biotechnology |
| Subjects: | |
| Online Access: | http://journals.sbmu.ac.ir/afb/article/view/28055 |
| _version_ | 1819099618229616640 |
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| author | Ashraf Hajhosseini Delaram Doroud Anoosheh Sharifan Zohreh Eftekhari |
| author_facet | Ashraf Hajhosseini Delaram Doroud Anoosheh Sharifan Zohreh Eftekhari |
| author_sort | Ashraf Hajhosseini |
| collection | DOAJ |
| description | Background and objective: Mannan which is a linear glycoprotein with β-1,4 links carrying mannose units bind to proteins, includes natural amphiphiles and serves as a bioemulsifier. The aim of this study was optimization of growth and purification of Kluyveromyces marxianus for mannan production, which can use as a natural bioemulisifier.
Material and methods: In this study, mannan production by Kluyveromyces marxianus was assessed using combinational method of fractional factorial design and response surface methodology optimization. Process variables include concentration of carbon source (15, 30, 45 g l-1) of glucose, and glycerol and methanol at 0, 2.5 and 5 gl-1), nitrogen source (yeast extract and peptone 4, 6 and 8 gl-1), as well as fermentation time (48, 96 and 144 h), pH (4, 6, 8) and agitation speed (150, 200 and 250 rpm).
Results and conclusion: Results showed that four variables of carbon and nitrogen source concentrations, as well as fermentation time and pH included the greatest effects on mannan production. Optimization of the affecting factors using response surface methodology demonstrated appropriate conditions of mannan production by Kluyveromyces marxianus as 55.15 g l-1 of glucose, 9.35 g l-1 of yeast extract, pH of 4.99 and fermentation time of 168 h, which led to a mannan yield of 245.98 mg (100 ml)-1culture media.
Conflict of interest: The authors declare no conflict of interest. |
| first_indexed | 2024-12-22T00:49:44Z |
| format | Article |
| id | doaj.art-70a6ae5c7f3b4ef6abade31c4663612e |
| institution | Directory Open Access Journal |
| issn | 2345-5357 2423-4214 |
| language | English |
| last_indexed | 2024-12-22T00:49:44Z |
| publishDate | 2020-03-01 |
| publisher | Shahid Behehsti University of Medical Sciences |
| record_format | Article |
| series | Applied Food Biotechnology |
| spelling | doaj.art-70a6ae5c7f3b4ef6abade31c4663612e2022-12-21T18:44:28ZengShahid Behehsti University of Medical SciencesApplied Food Biotechnology2345-53572423-42142020-03-017211512610.22037/afb.v7i2.2805513576Optimizing Growth Conditions of Kluyveromyces marxianus for Mannan Production as a BioemulsifierAshraf Hajhosseini0Delaram Doroud1Anoosheh Sharifan2Zohreh Eftekhari3Department of Food Science and Technology, Faculty of Agricultural Sciences and Food Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.Pasteur Institute of Iran, Tehran, Iran.Department of Food Science and Technology, Faculty of Agricultural Sciences and Food Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of quality control, Pasteur Institute, Tehran. Iran.Background and objective: Mannan which is a linear glycoprotein with β-1,4 links carrying mannose units bind to proteins, includes natural amphiphiles and serves as a bioemulsifier. The aim of this study was optimization of growth and purification of Kluyveromyces marxianus for mannan production, which can use as a natural bioemulisifier. Material and methods: In this study, mannan production by Kluyveromyces marxianus was assessed using combinational method of fractional factorial design and response surface methodology optimization. Process variables include concentration of carbon source (15, 30, 45 g l-1) of glucose, and glycerol and methanol at 0, 2.5 and 5 gl-1), nitrogen source (yeast extract and peptone 4, 6 and 8 gl-1), as well as fermentation time (48, 96 and 144 h), pH (4, 6, 8) and agitation speed (150, 200 and 250 rpm). Results and conclusion: Results showed that four variables of carbon and nitrogen source concentrations, as well as fermentation time and pH included the greatest effects on mannan production. Optimization of the affecting factors using response surface methodology demonstrated appropriate conditions of mannan production by Kluyveromyces marxianus as 55.15 g l-1 of glucose, 9.35 g l-1 of yeast extract, pH of 4.99 and fermentation time of 168 h, which led to a mannan yield of 245.98 mg (100 ml)-1culture media. Conflict of interest: The authors declare no conflict of interest.http://journals.sbmu.ac.ir/afb/article/view/28055▪ bioemulsifier ▪ kluyveromyces marxianus ▪ mannan ▪ purification ▪ production |
| spellingShingle | Ashraf Hajhosseini Delaram Doroud Anoosheh Sharifan Zohreh Eftekhari Optimizing Growth Conditions of Kluyveromyces marxianus for Mannan Production as a Bioemulsifier Applied Food Biotechnology ▪ bioemulsifier ▪ kluyveromyces marxianus ▪ mannan ▪ purification ▪ production |
| title | Optimizing Growth Conditions of Kluyveromyces marxianus for Mannan Production as a Bioemulsifier |
| title_full | Optimizing Growth Conditions of Kluyveromyces marxianus for Mannan Production as a Bioemulsifier |
| title_fullStr | Optimizing Growth Conditions of Kluyveromyces marxianus for Mannan Production as a Bioemulsifier |
| title_full_unstemmed | Optimizing Growth Conditions of Kluyveromyces marxianus for Mannan Production as a Bioemulsifier |
| title_short | Optimizing Growth Conditions of Kluyveromyces marxianus for Mannan Production as a Bioemulsifier |
| title_sort | optimizing growth conditions of kluyveromyces marxianus for mannan production as a bioemulsifier |
| topic | ▪ bioemulsifier ▪ kluyveromyces marxianus ▪ mannan ▪ purification ▪ production |
| url | http://journals.sbmu.ac.ir/afb/article/view/28055 |
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