Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors
Herbaceous peony (Paeonia lactiflora Pall.) is a famous flower with medicinal values, and its flowers have a number of medicinal constituents, especially flavonoids. In this study, a P. lactiflora cultivar with double colors including white outer-petal and yellow inner-petal was used as the experime...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-09-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915613181 |
| _version_ | 1818739091748945920 |
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| author | Yan-qing WU Meng-ran WEI Da-qiu ZHAO Jun TAO |
| author_facet | Yan-qing WU Meng-ran WEI Da-qiu ZHAO Jun TAO |
| author_sort | Yan-qing WU |
| collection | DOAJ |
| description | Herbaceous peony (Paeonia lactiflora Pall.) is a famous flower with medicinal values, and its flowers have a number of medicinal constituents, especially flavonoids. In this study, a P. lactiflora cultivar with double colors including white outer-petal and yellow inner-petal was used as the experimental materials to perform the qualitative and quantitative analysis of flavonoids by high-performance liquid chromatograph-electrospray ionization-mass spectrometry (HPLC-ESI-MSn) and investigate the expression patterns of flavonoid biosynthetic genes using real-time quantitative polymerase chain reaction (Q-PCR). The results showed that the colors of both petals gradually weakened with flower development. Moreover, one main anthocyanin composition (peonidin 3,5-di-O-glucoside) and five main anthoxanthin compositions (kaempferol di-hexo-side, kaempferol-3-O-malonylglucoside-7-O-glucoside, quercetin-3-O-galactoside, luteolin-7-O-glucoside and isorhamnetin-3-O-glucoside) were found in the both, differing significantly in their peak areas only. Total anthocyanin, anthoxanthin and flavonoid contents in white outer-petal and yellow inner-petal gradually decreased during flower development, and were consistently higher in white outer-petal. Furthermore, the expression patterns of nine structural genes in P. lactiflora flavonoid biosynthetic pathway showed that the expression levels of phenylalanine ammonialyase gene (PIPAL), chalcone synthase gene (PICHS), flavanone 3-hydroxylase gene (PIF3H), anthocyanidin synthase gene (PIANS) and UDP-glucoside: flavonoid 5-O-glucosyltransferase gene (PIF5GT) in two petals basically presented declined tendencies, and transcription levels of PIPAL, PICHS, PIANS, PIF3GT and PIF5GT also tended to be higher in white outer-petal, which was correlated with their flavonoid contents. These results would lay a solid foundation for the exploration and utilization of flavonoid resources in P. lactiflora flowers. |
| first_indexed | 2024-12-18T01:19:20Z |
| format | Article |
| id | doaj.art-70a8d61c508842b99bca6e7310c3e423 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T01:19:20Z |
| publishDate | 2016-09-01 |
| publisher | Elsevier |
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| series | Journal of Integrative Agriculture |
| spelling | doaj.art-70a8d61c508842b99bca6e7310c3e4232022-12-21T21:25:52ZengElsevierJournal of Integrative Agriculture2095-31192016-09-0115920232031Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colorsYan-qing WU0Meng-ran WEI1Da-qiu ZHAO2Jun TAO3Jiangsu Key Laboratory of Crop Genetics and Physiology/College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China; Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, P.R.ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China; Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, P.R.China; Correspondence TAO Jun, Tel: +86-514-87997219, Fax: +86-514-87347537Herbaceous peony (Paeonia lactiflora Pall.) is a famous flower with medicinal values, and its flowers have a number of medicinal constituents, especially flavonoids. In this study, a P. lactiflora cultivar with double colors including white outer-petal and yellow inner-petal was used as the experimental materials to perform the qualitative and quantitative analysis of flavonoids by high-performance liquid chromatograph-electrospray ionization-mass spectrometry (HPLC-ESI-MSn) and investigate the expression patterns of flavonoid biosynthetic genes using real-time quantitative polymerase chain reaction (Q-PCR). The results showed that the colors of both petals gradually weakened with flower development. Moreover, one main anthocyanin composition (peonidin 3,5-di-O-glucoside) and five main anthoxanthin compositions (kaempferol di-hexo-side, kaempferol-3-O-malonylglucoside-7-O-glucoside, quercetin-3-O-galactoside, luteolin-7-O-glucoside and isorhamnetin-3-O-glucoside) were found in the both, differing significantly in their peak areas only. Total anthocyanin, anthoxanthin and flavonoid contents in white outer-petal and yellow inner-petal gradually decreased during flower development, and were consistently higher in white outer-petal. Furthermore, the expression patterns of nine structural genes in P. lactiflora flavonoid biosynthetic pathway showed that the expression levels of phenylalanine ammonialyase gene (PIPAL), chalcone synthase gene (PICHS), flavanone 3-hydroxylase gene (PIF3H), anthocyanidin synthase gene (PIANS) and UDP-glucoside: flavonoid 5-O-glucosyltransferase gene (PIF5GT) in two petals basically presented declined tendencies, and transcription levels of PIPAL, PICHS, PIANS, PIF3GT and PIF5GT also tended to be higher in white outer-petal, which was correlated with their flavonoid contents. These results would lay a solid foundation for the exploration and utilization of flavonoid resources in P. lactiflora flowers.http://www.sciencedirect.com/science/article/pii/S2095311915613181herbaceous peonyflower colorflavonoidsgene expression |
| spellingShingle | Yan-qing WU Meng-ran WEI Da-qiu ZHAO Jun TAO Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors Journal of Integrative Agriculture herbaceous peony flower color flavonoids gene expression |
| title | Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors |
| title_full | Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors |
| title_fullStr | Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors |
| title_full_unstemmed | Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors |
| title_short | Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors |
| title_sort | flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony paeonia lactiflora pall with double colors |
| topic | herbaceous peony flower color flavonoids gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915613181 |
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