Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway

Oleuropein, a glycosylated secoiridoid present in olive leaves, is known to be an important antioxidant phenolic compound. We studied the antioxidant effect of low doses of oleuropein aglycone (3,4-DHPEA-EA) and oleuropein aglycone peracetylated (3,4-DHPEA-EA(P)) in murine C2C12 myocytes treated wit...

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Bibliographic Details
Main Authors: Monica Nardi, Sara Baldelli, Maria Rosa Ciriolo, Paola Costanzo, Antonio Procopio, Carmela Colica
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/25/22/5472
Description
Summary:Oleuropein, a glycosylated secoiridoid present in olive leaves, is known to be an important antioxidant phenolic compound. We studied the antioxidant effect of low doses of oleuropein aglycone (3,4-DHPEA-EA) and oleuropein aglycone peracetylated (3,4-DHPEA-EA(P)) in murine C2C12 myocytes treated with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Both compounds were used at a concentration of 10 μM and were able to inhibit cell death induced by the H<sub>2</sub>O<sub>2</sub> treatment, with 3,4-DHPEA-EA(P) being more. Under our experimental conditions, H<sub>2</sub>O<sub>2</sub> efficiently induced the phosphorylated-active form of JNK and of its downstream target c-Jun. We demonstrated, by Western blot analysis, that 3,4-DHPEA-EA(P) was efficient in inhibiting the phospho-active form of JNK. This data suggests that the growth arrest and cell death of C2C12 proceeds via the JNK/c-Jun pathway. Moreover, we demonstrated that 3,4-DHPEA-EA(P) affects the myogenesis of C2C12 cells; because MyoD mRNA levels and the differentiation process are restored with 3,4-DHPEA-EA(P) after treatment. Overall, the results indicate that 3,4-DHPEA-EA(P) prevents ROS-mediated degenerative process by functioning as an efficient antioxidant.
ISSN:1420-3049