Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway
Oleuropein, a glycosylated secoiridoid present in olive leaves, is known to be an important antioxidant phenolic compound. We studied the antioxidant effect of low doses of oleuropein aglycone (3,4-DHPEA-EA) and oleuropein aglycone peracetylated (3,4-DHPEA-EA(P)) in murine C2C12 myocytes treated wit...
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/22/5472 |
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| author | Monica Nardi Sara Baldelli Maria Rosa Ciriolo Paola Costanzo Antonio Procopio Carmela Colica |
| author_facet | Monica Nardi Sara Baldelli Maria Rosa Ciriolo Paola Costanzo Antonio Procopio Carmela Colica |
| author_sort | Monica Nardi |
| collection | DOAJ |
| description | Oleuropein, a glycosylated secoiridoid present in olive leaves, is known to be an important antioxidant phenolic compound. We studied the antioxidant effect of low doses of oleuropein aglycone (3,4-DHPEA-EA) and oleuropein aglycone peracetylated (3,4-DHPEA-EA(P)) in murine C2C12 myocytes treated with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Both compounds were used at a concentration of 10 μM and were able to inhibit cell death induced by the H<sub>2</sub>O<sub>2</sub> treatment, with 3,4-DHPEA-EA(P) being more. Under our experimental conditions, H<sub>2</sub>O<sub>2</sub> efficiently induced the phosphorylated-active form of JNK and of its downstream target c-Jun. We demonstrated, by Western blot analysis, that 3,4-DHPEA-EA(P) was efficient in inhibiting the phospho-active form of JNK. This data suggests that the growth arrest and cell death of C2C12 proceeds via the JNK/c-Jun pathway. Moreover, we demonstrated that 3,4-DHPEA-EA(P) affects the myogenesis of C2C12 cells; because MyoD mRNA levels and the differentiation process are restored with 3,4-DHPEA-EA(P) after treatment. Overall, the results indicate that 3,4-DHPEA-EA(P) prevents ROS-mediated degenerative process by functioning as an efficient antioxidant. |
| first_indexed | 2024-03-10T14:38:57Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:38:57Z |
| publishDate | 2020-11-01 |
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| spelling | doaj.art-70a9cb674d25483e9f081158746cb4082023-11-20T21:57:12ZengMDPI AGMolecules1420-30492020-11-012522547210.3390/molecules25225472Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling PathwayMonica Nardi0Sara Baldelli1Maria Rosa Ciriolo2Paola Costanzo3Antonio Procopio4Carmela Colica5Dipartimento di Scienze Della Salute, Università Magna Graecia, Viale Europa, 88100 Germaneto, ItalyDepartment of Human Sciences and Promotion of the Quality of Life, IRCCS San Raffaele Pisana, San Raffaele Roma Open University, 00163 Rome, ItalyDepartment of Biology, University of Rome “Tor Vergata”, 00133 Rome, ItalyDipartimento di Scienze Della Salute, Università Magna Graecia, Viale Europa, 88100 Germaneto, ItalyDipartimento di Scienze Della Salute, Università Magna Graecia, Viale Europa, 88100 Germaneto, ItalyCNR, IBFM UOS, Università Magna Graecia, Viale Europa, 88100 Germaneto, ItalyOleuropein, a glycosylated secoiridoid present in olive leaves, is known to be an important antioxidant phenolic compound. We studied the antioxidant effect of low doses of oleuropein aglycone (3,4-DHPEA-EA) and oleuropein aglycone peracetylated (3,4-DHPEA-EA(P)) in murine C2C12 myocytes treated with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Both compounds were used at a concentration of 10 μM and were able to inhibit cell death induced by the H<sub>2</sub>O<sub>2</sub> treatment, with 3,4-DHPEA-EA(P) being more. Under our experimental conditions, H<sub>2</sub>O<sub>2</sub> efficiently induced the phosphorylated-active form of JNK and of its downstream target c-Jun. We demonstrated, by Western blot analysis, that 3,4-DHPEA-EA(P) was efficient in inhibiting the phospho-active form of JNK. This data suggests that the growth arrest and cell death of C2C12 proceeds via the JNK/c-Jun pathway. Moreover, we demonstrated that 3,4-DHPEA-EA(P) affects the myogenesis of C2C12 cells; because MyoD mRNA levels and the differentiation process are restored with 3,4-DHPEA-EA(P) after treatment. Overall, the results indicate that 3,4-DHPEA-EA(P) prevents ROS-mediated degenerative process by functioning as an efficient antioxidant.https://www.mdpi.com/1420-3049/25/22/54723,4-DHPEA-EA derivativesC2C12 myocytesolive oilantioxidantskeletal muscle |
| spellingShingle | Monica Nardi Sara Baldelli Maria Rosa Ciriolo Paola Costanzo Antonio Procopio Carmela Colica Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway Molecules 3,4-DHPEA-EA derivatives C2C12 myocytes olive oil antioxidant skeletal muscle |
| title | Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway |
| title_full | Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway |
| title_fullStr | Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway |
| title_full_unstemmed | Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway |
| title_short | Oleuropein Aglycone Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway |
| title_sort | oleuropein aglycone peracetylated 3 4 dhpea ea p attenuates h sub 2 sub o sub 2 sub mediated cytotoxicity in c2c12 myocytes via inactivation of p jnk p c jun signaling pathway |
| topic | 3,4-DHPEA-EA derivatives C2C12 myocytes olive oil antioxidant skeletal muscle |
| url | https://www.mdpi.com/1420-3049/25/22/5472 |
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