Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM.
Timely completion of eukaryotic genome duplication requires coordinated DNA replication initiation at multiple origins. Replication begins with the loading of the Mini-Chromosome Maintenance (MCM) complex, proceeds by the activation of the Cdc45-MCM-GINS (CMG) helicase, and ends with CMG removal aft...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-06-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010275 |
| _version_ | 1818193538318336000 |
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| author | Yun Quan Qian-Yi Zhang Ann L Zhou Yuhao Wang Jiaxi Cai Yong-Qi Gao Huilin Zhou |
| author_facet | Yun Quan Qian-Yi Zhang Ann L Zhou Yuhao Wang Jiaxi Cai Yong-Qi Gao Huilin Zhou |
| author_sort | Yun Quan |
| collection | DOAJ |
| description | Timely completion of eukaryotic genome duplication requires coordinated DNA replication initiation at multiple origins. Replication begins with the loading of the Mini-Chromosome Maintenance (MCM) complex, proceeds by the activation of the Cdc45-MCM-GINS (CMG) helicase, and ends with CMG removal after chromosomes are fully replicated. Post-translational modifications on the MCM and associated factors ensure an orderly transit of these steps. Although the mechanisms of CMG activation and removal are partially understood, regulated MCM loading is not, leaving an incomplete understanding of how DNA replication begins. Here we describe a site-specific modification of Mcm3 by the Small Ubiquitin-like MOdifier (SUMO). Mutations that prevent this modification reduce the MCM loaded at replication origins and lower CMG levels, resulting in impaired cell growth, delayed chromosomal replication, and the accumulation of gross chromosomal rearrangements (GCRs). These findings demonstrate the existence of a SUMO-dependent regulation of origin-bound MCM and show that this pathway is needed to prevent genome rearrangements. |
| first_indexed | 2024-12-12T00:47:59Z |
| format | Article |
| id | doaj.art-70aa52487394453ab2f319c0907e71f3 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-12T00:47:59Z |
| publishDate | 2022-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-70aa52487394453ab2f319c0907e71f32022-12-22T00:44:04ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042022-06-01186e101027510.1371/journal.pgen.1010275Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM.Yun QuanQian-Yi ZhangAnn L ZhouYuhao WangJiaxi CaiYong-Qi GaoHuilin ZhouTimely completion of eukaryotic genome duplication requires coordinated DNA replication initiation at multiple origins. Replication begins with the loading of the Mini-Chromosome Maintenance (MCM) complex, proceeds by the activation of the Cdc45-MCM-GINS (CMG) helicase, and ends with CMG removal after chromosomes are fully replicated. Post-translational modifications on the MCM and associated factors ensure an orderly transit of these steps. Although the mechanisms of CMG activation and removal are partially understood, regulated MCM loading is not, leaving an incomplete understanding of how DNA replication begins. Here we describe a site-specific modification of Mcm3 by the Small Ubiquitin-like MOdifier (SUMO). Mutations that prevent this modification reduce the MCM loaded at replication origins and lower CMG levels, resulting in impaired cell growth, delayed chromosomal replication, and the accumulation of gross chromosomal rearrangements (GCRs). These findings demonstrate the existence of a SUMO-dependent regulation of origin-bound MCM and show that this pathway is needed to prevent genome rearrangements.https://doi.org/10.1371/journal.pgen.1010275 |
| spellingShingle | Yun Quan Qian-Yi Zhang Ann L Zhou Yuhao Wang Jiaxi Cai Yong-Qi Gao Huilin Zhou Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM. PLoS Genetics |
| title | Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM. |
| title_full | Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM. |
| title_fullStr | Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM. |
| title_full_unstemmed | Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM. |
| title_short | Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM. |
| title_sort | site specific mcm sumoylation prevents genome rearrangements by controlling origin bound mcm |
| url | https://doi.org/10.1371/journal.pgen.1010275 |
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