Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons
Small-angle X-ray scattering (SAXS) is an established technique that provides low-resolution structural information on macromolecular solutions. Recent decades have witnessed significant progress in both experimental facilities and in novel data-analysis approaches, making SAXS a mainstream method f...
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| Format: | Article |
| Language: | English |
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International Union of Crystallography
2017-09-01
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| Series: | IUCrJ |
| Subjects: | |
| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S2052252517008740 |
| _version_ | 1818941533681876992 |
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| author | Anne T. Tuukkanen Alessandro Spilotros Dmitri I. Svergun |
| author_facet | Anne T. Tuukkanen Alessandro Spilotros Dmitri I. Svergun |
| author_sort | Anne T. Tuukkanen |
| collection | DOAJ |
| description | Small-angle X-ray scattering (SAXS) is an established technique that provides low-resolution structural information on macromolecular solutions. Recent decades have witnessed significant progress in both experimental facilities and in novel data-analysis approaches, making SAXS a mainstream method for structural biology. The technique is routinely applied to directly reconstruct low-resolution shapes of proteins and to generate atomistic models of macromolecular assemblies using hybrid approaches. Very importantly, SAXS is capable of yielding structural information on systems with size and conformational polydispersity, including highly flexible objects. In addition, utilizing high-flux synchrotron facilities, time-resolved SAXS allows analysis of kinetic processes over time ranges from microseconds to hours. Dedicated bioSAXS beamlines now offer fully automated data-collection and analysis pipelines, where analysis and modelling is conducted on the fly. This enables SAXS to be employed as a high-throughput method to rapidly screen various sample conditions and additives. The growing SAXS user community is supported by developments in data and model archiving and quality criteria. This review illustrates the latest developments in SAXS, in particular highlighting time-resolved applications aimed at flexible and evolving systems. |
| first_indexed | 2024-12-20T06:57:03Z |
| format | Article |
| id | doaj.art-70aba78db1f34878a622fe02d7467d0b |
| institution | Directory Open Access Journal |
| issn | 2052-2525 |
| language | English |
| last_indexed | 2024-12-20T06:57:03Z |
| publishDate | 2017-09-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | IUCrJ |
| spelling | doaj.art-70aba78db1f34878a622fe02d7467d0b2022-12-21T19:49:19ZengInternational Union of CrystallographyIUCrJ2052-25252017-09-014551852810.1107/S2052252517008740tj5011Progress in small-angle scattering from biological solutions at high-brilliance synchrotronsAnne T. Tuukkanen0Alessandro Spilotros1Dmitri I. Svergun2European Molecular Biology Laboratory, EMBL Hamburg c/o DESY, Notkestrasse 85, 22607 Hamburg, GermanyEuropean Molecular Biology Laboratory, EMBL Hamburg c/o DESY, Notkestrasse 85, 22607 Hamburg, GermanyEuropean Molecular Biology Laboratory, EMBL Hamburg c/o DESY, Notkestrasse 85, 22607 Hamburg, GermanySmall-angle X-ray scattering (SAXS) is an established technique that provides low-resolution structural information on macromolecular solutions. Recent decades have witnessed significant progress in both experimental facilities and in novel data-analysis approaches, making SAXS a mainstream method for structural biology. The technique is routinely applied to directly reconstruct low-resolution shapes of proteins and to generate atomistic models of macromolecular assemblies using hybrid approaches. Very importantly, SAXS is capable of yielding structural information on systems with size and conformational polydispersity, including highly flexible objects. In addition, utilizing high-flux synchrotron facilities, time-resolved SAXS allows analysis of kinetic processes over time ranges from microseconds to hours. Dedicated bioSAXS beamlines now offer fully automated data-collection and analysis pipelines, where analysis and modelling is conducted on the fly. This enables SAXS to be employed as a high-throughput method to rapidly screen various sample conditions and additives. The growing SAXS user community is supported by developments in data and model archiving and quality criteria. This review illustrates the latest developments in SAXS, in particular highlighting time-resolved applications aimed at flexible and evolving systems.http://scripts.iucr.org/cgi-bin/paper?S2052252517008740small-angle X-ray scatteringstructural modellingtime-resolved SAXS |
| spellingShingle | Anne T. Tuukkanen Alessandro Spilotros Dmitri I. Svergun Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons IUCrJ small-angle X-ray scattering structural modelling time-resolved SAXS |
| title | Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons |
| title_full | Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons |
| title_fullStr | Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons |
| title_full_unstemmed | Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons |
| title_short | Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons |
| title_sort | progress in small angle scattering from biological solutions at high brilliance synchrotrons |
| topic | small-angle X-ray scattering structural modelling time-resolved SAXS |
| url | http://scripts.iucr.org/cgi-bin/paper?S2052252517008740 |
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