SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway
Background: Spi-B transcription factor (SPIB) is an E-twenty-six (ETS) transcription factor associated with tumor immunity.Objective: To investigate the functions and mechanisms of SPIB in ovarian cancer (OC) cells.Methods: Cell proliferation, apoptosis, migration, and invasion were determined using...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2023-09-01
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| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | https://iji.sums.ac.ir/article_49417_eb2b44281a202c059b92e4db32978344.pdf |
| _version_ | 1797680495050358784 |
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| author | Wenfei Gu GuangTao Qi Li Chen |
| author_facet | Wenfei Gu GuangTao Qi Li Chen |
| author_sort | Wenfei Gu |
| collection | DOAJ |
| description | Background: Spi-B transcription factor (SPIB) is an E-twenty-six (ETS) transcription factor associated with tumor immunity.Objective: To investigate the functions and mechanisms of SPIB in ovarian cancer (OC) cells.Methods: Cell proliferation, apoptosis, migration, and invasion were determined using colony formation, EdU, flow cytometry, and transwell assays, respectively. The binding sites of programmed death-ligand 1 (PD-L1) and SPIB were predicted using the JASPAR database and verified using the ChIP and luciferase reporter assays.Results: SPIB knockdown inhibited OC cell proliferation, migration, and invasion, and significantly boosted apoptosis (p<0.05). SPIB directly enhanced PD-L1 transcription in OVCAR-3 and SKOV3 cells (p<0.05). Importantly, the JAK/STAT pathway was markedly inactivated in OC cells upon SPIB knockdown. SPIB knockdown markedly decreased JAK2 and STAT1 phosphorylation in OVCAR-3 and SKOV3 cells (p<0.05).Conclusion: These data indicate that SPIB knockdown inhibits OC cell progression by downregulating PD-L1 and inactivating the JAK/STAT pathway. |
| first_indexed | 2024-03-11T23:30:49Z |
| format | Article |
| id | doaj.art-70ae7603176b4549998829f8f78f71d4 |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-03-11T23:30:49Z |
| publishDate | 2023-09-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-70ae7603176b4549998829f8f78f71d42023-09-20T09:13:41ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2023-09-0120333534710.22034/iji.2023.98236.255949417SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT PathwayWenfei Gu0GuangTao Qi1Li Chen2Department of Gynecology, Binzhou Medical University Hospital, Binzhou, Shandong, 256600, China.Department of Gynecology, Binzhou Medical University Hospital, Binzhou, Shandong, 256600, China.Department of Gynecology, Binzhou Medical University Hospital, Binzhou, Shandong, 256600, China.Background: Spi-B transcription factor (SPIB) is an E-twenty-six (ETS) transcription factor associated with tumor immunity.Objective: To investigate the functions and mechanisms of SPIB in ovarian cancer (OC) cells.Methods: Cell proliferation, apoptosis, migration, and invasion were determined using colony formation, EdU, flow cytometry, and transwell assays, respectively. The binding sites of programmed death-ligand 1 (PD-L1) and SPIB were predicted using the JASPAR database and verified using the ChIP and luciferase reporter assays.Results: SPIB knockdown inhibited OC cell proliferation, migration, and invasion, and significantly boosted apoptosis (p<0.05). SPIB directly enhanced PD-L1 transcription in OVCAR-3 and SKOV3 cells (p<0.05). Importantly, the JAK/STAT pathway was markedly inactivated in OC cells upon SPIB knockdown. SPIB knockdown markedly decreased JAK2 and STAT1 phosphorylation in OVCAR-3 and SKOV3 cells (p<0.05).Conclusion: These data indicate that SPIB knockdown inhibits OC cell progression by downregulating PD-L1 and inactivating the JAK/STAT pathway.https://iji.sums.ac.ir/article_49417_eb2b44281a202c059b92e4db32978344.pdfjak/stat pathwayovarian cancerpd-l1 (cd274)spib |
| spellingShingle | Wenfei Gu GuangTao Qi Li Chen SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway Iranian Journal of Immunology jak/stat pathway ovarian cancer pd-l1 (cd274) spib |
| title | SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway |
| title_full | SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway |
| title_fullStr | SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway |
| title_full_unstemmed | SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway |
| title_short | SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway |
| title_sort | spib knockdown inhibits the immune escape of ovarian cancer cells by reducing pd l1 cd274 expression and inactivating the jak stat pathway |
| topic | jak/stat pathway ovarian cancer pd-l1 (cd274) spib |
| url | https://iji.sums.ac.ir/article_49417_eb2b44281a202c059b92e4db32978344.pdf |
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