Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitis
To assess the clinical impact, epidemiology of intra-mammary infections, accurate diagnosis, and choosingappropriate antimicrobial therapy, the staphylococci group should be studied correctly up to the species level. Inthis study, a multiplex PCR (mPCR) was developed to identify 10 important Staphyl...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2022-02-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/120909 |
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| author | TAWHEED AHMAD SHAFI BALJINDER KUMAR BANSAL SHUKRITI SHARMA NARINDER SINGH SHARMA |
| author_facet | TAWHEED AHMAD SHAFI BALJINDER KUMAR BANSAL SHUKRITI SHARMA NARINDER SINGH SHARMA |
| author_sort | TAWHEED AHMAD SHAFI |
| collection | DOAJ |
| description | To assess the clinical impact, epidemiology of intra-mammary infections, accurate diagnosis, and choosingappropriate antimicrobial therapy, the staphylococci group should be studied correctly up to the species level. Inthis study, a multiplex PCR (mPCR) was developed to identify 10 important Staphylococcus species that wasapplied to study the prevalence of staphylococci species in bovine mastitis from five agro-climatic regions ofPunjab, India. A two tube mPCR assay consisted of 5 species each, with one reaction including primer pairs ofS. epidermidis, S. hemolyticus, S. hominis, S. warneri and S. saprophyticus, and another reaction consisted primerpairs of S. aureus, S. intermedius, S. chromogenes, S. sciuri and S. capitis was standardized. Prevalence studyrevealed S. aureus (35.76%) as most prevalent species among all staphylococci followed by S. chromogenes (16.56%),S. haemolyticus (15.23%), S. epidermidis (5.30%), S. sciuri (1.99%), S. capitis (0.66%), S. hominis (0.66%),S. saprophyticus (0.66%) and rest were other staphylococci (23.18%). The mPCR developed in this study could beused for identification of staphylococci of various origins including those from human specimens. The high prevalenceof S. chromogenes and S. haemolyticus in the present study suggest emergence of these non-aureus staphylococciin bovine mastitis in Punjab (India). |
| first_indexed | 2024-04-10T16:35:41Z |
| format | Article |
| id | doaj.art-70b3a700141341d7a0cb25fc3cbe2268 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T16:35:41Z |
| publishDate | 2022-02-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-70b3a700141341d7a0cb25fc3cbe22682023-02-08T11:30:24ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272022-02-0192110.56093/ijans.v92i1.120909Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitisTAWHEED AHMAD SHAFI0BALJINDER KUMAR BANSAL1SHUKRITI SHARMA2NARINDER SINGH SHARMA3Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaTo assess the clinical impact, epidemiology of intra-mammary infections, accurate diagnosis, and choosingappropriate antimicrobial therapy, the staphylococci group should be studied correctly up to the species level. Inthis study, a multiplex PCR (mPCR) was developed to identify 10 important Staphylococcus species that wasapplied to study the prevalence of staphylococci species in bovine mastitis from five agro-climatic regions ofPunjab, India. A two tube mPCR assay consisted of 5 species each, with one reaction including primer pairs ofS. epidermidis, S. hemolyticus, S. hominis, S. warneri and S. saprophyticus, and another reaction consisted primerpairs of S. aureus, S. intermedius, S. chromogenes, S. sciuri and S. capitis was standardized. Prevalence studyrevealed S. aureus (35.76%) as most prevalent species among all staphylococci followed by S. chromogenes (16.56%),S. haemolyticus (15.23%), S. epidermidis (5.30%), S. sciuri (1.99%), S. capitis (0.66%), S. hominis (0.66%),S. saprophyticus (0.66%) and rest were other staphylococci (23.18%). The mPCR developed in this study could beused for identification of staphylococci of various origins including those from human specimens. The high prevalenceof S. chromogenes and S. haemolyticus in the present study suggest emergence of these non-aureus staphylococciin bovine mastitis in Punjab (India).https://epubs.icar.org.in/index.php/IJAnS/article/view/120909Bovine mastitisMultiplex PCRNon-aureus staphylococci |
| spellingShingle | TAWHEED AHMAD SHAFI BALJINDER KUMAR BANSAL SHUKRITI SHARMA NARINDER SINGH SHARMA Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitis Indian Journal of Animal Sciences Bovine mastitis Multiplex PCR Non-aureus staphylococci |
| title | Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitis |
| title_full | Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitis |
| title_fullStr | Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitis |
| title_full_unstemmed | Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitis |
| title_short | Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitis |
| title_sort | standardization of multiplex pcr for identification of ten staphylococci species and their prevalence in bovine mastitis |
| topic | Bovine mastitis Multiplex PCR Non-aureus staphylococci |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/120909 |
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