Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144
The evaluation of glycosyl composition is an essential step to guide future research designs applied in bioactivity. In the same way, the unexplored potential bioactivity of exopolysaccharide from Ganoderma lucidum is huge. Therefore, this study investigated the glycosyl composition of the exopolysa...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-07-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/3427 |
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| author | Rosália Rubel Leandro Freire dos Santos Herta Stutz Dalla Santa Luciana Porto de Souza Vandenberghe Adenise Lorenci Woiciechowski Júlio Cesar de Carvalho Sascha Habu Francisco Menino Destéfanis Vítola Christopher Augur Carlos Ricardo Soccol |
| author_facet | Rosália Rubel Leandro Freire dos Santos Herta Stutz Dalla Santa Luciana Porto de Souza Vandenberghe Adenise Lorenci Woiciechowski Júlio Cesar de Carvalho Sascha Habu Francisco Menino Destéfanis Vítola Christopher Augur Carlos Ricardo Soccol |
| author_sort | Rosália Rubel |
| collection | DOAJ |
| description | The evaluation of glycosyl composition is an essential step to guide future research designs applied in bioactivity. In the same way, the unexplored potential bioactivity of exopolysaccharide from Ganoderma lucidum is huge. Therefore, this study investigated the glycosyl composition of the exopolysaccharide isolated from submerged fermentation of G. lucidum to serve as guide for future studies on bioactivity. Glycosyl content and composition were evaluated by combined GC/MS of the TMS derivatives of the monosaccharide methyl glycosides produced from the sample by acidic methanolysis. Glycosyl composition analysis showed that the dominant carbohydrate component in all samples of exopolysaccharide isolated from submerged fermentation of G. lucidum CG 144 was glucose (58.1%), mannose (26.6%) and galactose (12.5%) which can be referred to as heteroglycan. These results suggest that this Ganoderma exopolysaccharide may be a new immunomodulatory agent. |
| first_indexed | 2024-12-12T18:18:32Z |
| format | Article |
| id | doaj.art-70b470c818504c90bbc3dd5b652e960c |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-12T18:18:32Z |
| publishDate | 2014-07-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-70b470c818504c90bbc3dd5b652e960c2022-12-22T00:16:12ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-07-0183323924110.5586/asbp.2014.0202944Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144Rosália Rubel0Leandro Freire dos Santos1Herta Stutz Dalla Santa2Luciana Porto de Souza Vandenberghe3Adenise Lorenci Woiciechowski4Júlio Cesar de Carvalho5Sascha Habu6Francisco Menino Destéfanis Vítola7Christopher Augur8Carlos Ricardo Soccol9Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)Institute of Research for Development, Unite Biotrans – IMEP, Boite 441, Paul Cézanne University, Av. Escadrille Normandie-Niemen, F-13391 Marseille Cedex 20Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), Jardim das Américas, CEP 81531-990, Curitiba, Paraná (PR)The evaluation of glycosyl composition is an essential step to guide future research designs applied in bioactivity. In the same way, the unexplored potential bioactivity of exopolysaccharide from Ganoderma lucidum is huge. Therefore, this study investigated the glycosyl composition of the exopolysaccharide isolated from submerged fermentation of G. lucidum to serve as guide for future studies on bioactivity. Glycosyl content and composition were evaluated by combined GC/MS of the TMS derivatives of the monosaccharide methyl glycosides produced from the sample by acidic methanolysis. Glycosyl composition analysis showed that the dominant carbohydrate component in all samples of exopolysaccharide isolated from submerged fermentation of G. lucidum CG 144 was glucose (58.1%), mannose (26.6%) and galactose (12.5%) which can be referred to as heteroglycan. These results suggest that this Ganoderma exopolysaccharide may be a new immunomodulatory agent.https://pbsociety.org.pl/journals/index.php/asbp/article/view/3427glycosyl compositionexopolysaccharideGanoderma lucidumsubmerged fermentationheteroglycan |
| spellingShingle | Rosália Rubel Leandro Freire dos Santos Herta Stutz Dalla Santa Luciana Porto de Souza Vandenberghe Adenise Lorenci Woiciechowski Júlio Cesar de Carvalho Sascha Habu Francisco Menino Destéfanis Vítola Christopher Augur Carlos Ricardo Soccol Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144 Acta Societatis Botanicorum Poloniae glycosyl composition exopolysaccharide Ganoderma lucidum submerged fermentation heteroglycan |
| title | Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144 |
| title_full | Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144 |
| title_fullStr | Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144 |
| title_full_unstemmed | Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144 |
| title_short | Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144 |
| title_sort | analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of ganoderma lucidum cg144 |
| topic | glycosyl composition exopolysaccharide Ganoderma lucidum submerged fermentation heteroglycan |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/3427 |
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