The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus
Pandanus tectorius leaf extract effect on the White-leg shrimp Penaeus vannamei tolerance against Vibrio parahaemolyticus were investigated in this study. Thirty shrimp post-larvae measured at approximately 1 cm were exposed for 24 h to 0.5, 1, 2, 3, 4, 5 and 6 g/L leaf extract and subsequently obse...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Fish and Shellfish Immunology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266701192300021X |
| _version_ | 1797799898993655808 |
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| author | Anupa Anirudhan Mat Taib Mimi Iryani Yosie Andriani Patrick Sorgeloos Min Pau Tan Li Lian Wong Wen Jye Mok Wang Ming Liang Yantao Cher Chien Lau Yeong Yik Sung |
| author_facet | Anupa Anirudhan Mat Taib Mimi Iryani Yosie Andriani Patrick Sorgeloos Min Pau Tan Li Lian Wong Wen Jye Mok Wang Ming Liang Yantao Cher Chien Lau Yeong Yik Sung |
| author_sort | Anupa Anirudhan |
| collection | DOAJ |
| description | Pandanus tectorius leaf extract effect on the White-leg shrimp Penaeus vannamei tolerance against Vibrio parahaemolyticus were investigated in this study. Thirty shrimp post-larvae measured at approximately 1 cm were exposed for 24 h to 0.5, 1, 2, 3, 4, 5 and 6 g/L leaf extract and subsequently observed for survival and immune-related genes expression (Hsp70, ProPO, peroxinectin, penaeidin, crustin and transglutaminase), followed by determination of their tolerance and histological tissue profiles upon Vibrio challenge. Survival of shrimps treated with 6 g/L of leaf extract improved by up to 95% to controls. Hsp70, crustin, and prophenoloxidase mRNA levels were observed to be 8.5, 10.4, and 1.5-fold higher, respectively. Histopathological analysis of the hepatopancreas and the muscle tissues revealed major tissue degeneration in Vibrio-challenged shrimps but not in shrimps primed with P. tectorius leaf extract. Of all the dose examined, the best pathogen resistance results were obtained with a 24 h incubation of shrimp in 6 g/L P. tectorius methanolic leaf extract. The tolerance towards V. parahaemolyticus might be associated with the increased regulation of Hsp70, prophenoloxidase and crustin upon exposure to the extract, all immune-related proteins essential for pathogen elimination in Penaeid shrimp. The present study primarily demonstrated that P. tectorius leaf extract is a viable alternative for enhancing P. vannamei post-larvae resistance against V. parahaemolyticus, a major bacterial pathogen in aquaculture. |
| first_indexed | 2024-03-13T04:26:55Z |
| format | Article |
| id | doaj.art-70b4ee8b066b49e8831b931f0540cecc |
| institution | Directory Open Access Journal |
| issn | 2667-0119 |
| language | English |
| last_indexed | 2024-03-13T04:26:55Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Fish and Shellfish Immunology Reports |
| spelling | doaj.art-70b4ee8b066b49e8831b931f0540cecc2023-06-20T04:21:24ZengElsevierFish and Shellfish Immunology Reports2667-01192023-12-014100101The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticusAnupa Anirudhan0Mat Taib Mimi Iryani1Yosie Andriani2Patrick Sorgeloos3Min Pau Tan4Li Lian Wong5Wen Jye Mok6Wang Ming7Liang Yantao8Cher Chien Lau9Yeong Yik Sung10Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia; Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia; Laboratory of Aquaculture and Artemia Reference Center, Department of Animal Sciences and Aquatic Ecology, Campus Coupure - Blok F, Ghent University, Coupure Links 653, Gent B-9000, BelgiumInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia; UMT-OUC Joint Academic Center for Marine Studies, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia; UMT-OUC Joint Academic Center for Marine Studies, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China; UMT-OUC Joint Academic Center for Marine Studies, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaCollege of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China; UMT-OUC Joint Academic Center for Marine Studies, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia; Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia; UMT-OUC Joint Academic Center for Marine Studies, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia; Corresponding author at: Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia.Pandanus tectorius leaf extract effect on the White-leg shrimp Penaeus vannamei tolerance against Vibrio parahaemolyticus were investigated in this study. Thirty shrimp post-larvae measured at approximately 1 cm were exposed for 24 h to 0.5, 1, 2, 3, 4, 5 and 6 g/L leaf extract and subsequently observed for survival and immune-related genes expression (Hsp70, ProPO, peroxinectin, penaeidin, crustin and transglutaminase), followed by determination of their tolerance and histological tissue profiles upon Vibrio challenge. Survival of shrimps treated with 6 g/L of leaf extract improved by up to 95% to controls. Hsp70, crustin, and prophenoloxidase mRNA levels were observed to be 8.5, 10.4, and 1.5-fold higher, respectively. Histopathological analysis of the hepatopancreas and the muscle tissues revealed major tissue degeneration in Vibrio-challenged shrimps but not in shrimps primed with P. tectorius leaf extract. Of all the dose examined, the best pathogen resistance results were obtained with a 24 h incubation of shrimp in 6 g/L P. tectorius methanolic leaf extract. The tolerance towards V. parahaemolyticus might be associated with the increased regulation of Hsp70, prophenoloxidase and crustin upon exposure to the extract, all immune-related proteins essential for pathogen elimination in Penaeid shrimp. The present study primarily demonstrated that P. tectorius leaf extract is a viable alternative for enhancing P. vannamei post-larvae resistance against V. parahaemolyticus, a major bacterial pathogen in aquaculture.http://www.sciencedirect.com/science/article/pii/S266701192300021XPenaeus vannameiPandanus tectoriusVibrioImmunityHsp70 |
| spellingShingle | Anupa Anirudhan Mat Taib Mimi Iryani Yosie Andriani Patrick Sorgeloos Min Pau Tan Li Lian Wong Wen Jye Mok Wang Ming Liang Yantao Cher Chien Lau Yeong Yik Sung The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus Fish and Shellfish Immunology Reports Penaeus vannamei Pandanus tectorius Vibrio Immunity Hsp70 |
| title | The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus |
| title_full | The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus |
| title_fullStr | The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus |
| title_full_unstemmed | The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus |
| title_short | The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus |
| title_sort | effects of pandanus tectorius leaf extract on the resistance of white leg shrimp penaeus vannamei towards pathogenic vibrio parahaemolyticus |
| topic | Penaeus vannamei Pandanus tectorius Vibrio Immunity Hsp70 |
| url | http://www.sciencedirect.com/science/article/pii/S266701192300021X |
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