RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains
Restriction fragment length polymorphism (RFLP) analysis of a PCR-amplified fragment of the 16S rRNA gene was performed on reference strains belonging to 21 different enterococcal species and on 75 Enterococcus isolates recovered from poultry meat, pasteurised milk and fresh cheese. PCR amplificatio...
| Autors principals: | , , , |
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| Format: | Article |
| Idioma: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2009-11-01
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| Col·lecció: | Memorias do Instituto Oswaldo Cruz |
| Matèries: | |
| Accés en línia: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700011 |
| _version_ | 1827833161083518976 |
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| author | EMD Scheidegger SAP Fracalanzza LM Teixeira P Cardarelli-Leite |
| author_facet | EMD Scheidegger SAP Fracalanzza LM Teixeira P Cardarelli-Leite |
| author_sort | EMD Scheidegger |
| collection | DOAJ |
| description | Restriction fragment length polymorphism (RFLP) analysis of a PCR-amplified fragment of the 16S rRNA gene was performed on reference strains belonging to 21 different enterococcal species and on 75 Enterococcus isolates recovered from poultry meat, pasteurised milk and fresh cheese. PCR amplification generated a 275 bp fragment, which was digested with three restriction endonucleases (DdeI, HaeIII, HinfI). The strains were divided into five groups (groups A-E) on the basis of their restriction patterns. Five biochemical tests (arabinose, arginine, manitol, methyl-β-D-glucopyranoside and raffinose) were then performed in addition to RFLP analysis to narrow the identification of enterococcal strains to the species level. PCR-RFLP, in conjunction with the selected biochemical tests, allowed the precise identification of the 21 species of Enterococcus included in the present study. This proposed method is relatively simple and rapid and can be useful as an adjunct tool for accurate identification of Enterococcus. |
| first_indexed | 2024-03-12T05:25:50Z |
| format | Article |
| id | doaj.art-70bd901b543f4bdb8995ecd524e33bc7 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:25:50Z |
| publishDate | 2009-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-70bd901b543f4bdb8995ecd524e33bc72023-09-03T07:24:29ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602009-11-0110471003100810.1590/S0074-02762009000700011RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strainsEMD ScheideggerSAP FracalanzzaLM TeixeiraP Cardarelli-LeiteRestriction fragment length polymorphism (RFLP) analysis of a PCR-amplified fragment of the 16S rRNA gene was performed on reference strains belonging to 21 different enterococcal species and on 75 Enterococcus isolates recovered from poultry meat, pasteurised milk and fresh cheese. PCR amplification generated a 275 bp fragment, which was digested with three restriction endonucleases (DdeI, HaeIII, HinfI). The strains were divided into five groups (groups A-E) on the basis of their restriction patterns. Five biochemical tests (arabinose, arginine, manitol, methyl-β-D-glucopyranoside and raffinose) were then performed in addition to RFLP analysis to narrow the identification of enterococcal strains to the species level. PCR-RFLP, in conjunction with the selected biochemical tests, allowed the precise identification of the 21 species of Enterococcus included in the present study. This proposed method is relatively simple and rapid and can be useful as an adjunct tool for accurate identification of Enterococcus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-0276200900070001116S rRNA gene PCR-RFLPEnterococcus identificationfood isolatesPCR-based identificationphenotypical characterisation |
| spellingShingle | EMD Scheidegger SAP Fracalanzza LM Teixeira P Cardarelli-Leite RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains Memorias do Instituto Oswaldo Cruz 16S rRNA gene PCR-RFLP Enterococcus identification food isolates PCR-based identification phenotypical characterisation |
| title | RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains |
| title_full | RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains |
| title_fullStr | RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains |
| title_full_unstemmed | RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains |
| title_short | RFLP analysis of a PCR-amplified fragment of the 16S rRNA gene as a tool to identify Enterococcus strains |
| title_sort | rflp analysis of a pcr amplified fragment of the 16s rrna gene as a tool to identify enterococcus strains |
| topic | 16S rRNA gene PCR-RFLP Enterococcus identification food isolates PCR-based identification phenotypical characterisation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700011 |
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