Apoptotic mechanisms in rabbits with blast-induced acute lung injury
Abstract Purpose: To investigate the apoptotic mechanisms in rabbits with blast-induced acute lung injury (ALI). Methods: A total of 40 rabbits were randomly divided into a blank control group (A, n=10) and an experimental group (EXP, n=30). Explosion-induced chest-ALI models were prepared and sa...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2018-10-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/acb/v33n10/1678-2674-acb-33-10-00896.pdf |
| _version_ | 1818491001905348608 |
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| author | Xiao-Lin Qi Jian Hao Lian-Jiang Huang Sheng Wu Hong-Hao Ma Zhang-Qing Ye Hui-Bo He Shu-Wen Li Cheng-En Li Xin Huang |
| author_facet | Xiao-Lin Qi Jian Hao Lian-Jiang Huang Sheng Wu Hong-Hao Ma Zhang-Qing Ye Hui-Bo He Shu-Wen Li Cheng-En Li Xin Huang |
| author_sort | Xiao-Lin Qi |
| collection | DOAJ |
| description | Abstract Purpose: To investigate the apoptotic mechanisms in rabbits with blast-induced acute lung injury (ALI). Methods: A total of 40 rabbits were randomly divided into a blank control group (A, n=10) and an experimental group (EXP, n=30). Explosion-induced chest-ALI models were prepared and sampled at different time points (4, 12, and 24h after modeling, T1-T3) to test the lung dry weight/wet weight ratio (W/D) and arterial oxygen pressure (PaO2), apoptosis of lung tissue by the TUNEL assay, and Caspase-3, Bax, and Bcl-2 levels by immunohistochemical analysis. Furthermore, lung tissue was sampled to observe pathological morphology by microscopy. Results: Under a light microscope, Group EXP exhibited obvious edema in the pulmonary interstitial substance and alveoli, a large number of red blood cells, inflammatory cells, and serous exudation in the alveolar cavity, as well as thickening of the pulmonary interstitial fluid. Compared to Group A, the W/D ratio was significantly increased in Group EXP (P<0.01), while PaO2 was significantly reduced (P<0.01). The apoptosis index was significantly increased (P<0.01), and caspase-3 and Bax/Bcl-2 levels were increased (P<0.01). Conclusion: Apoptosis plays an important role in the occurrence and development of acute lung injury in rabbits by participating in lung injury and promoting the progression of ALI. |
| first_indexed | 2024-12-10T17:24:29Z |
| format | Article |
| id | doaj.art-70bef7bae1e04f18a8311620b975ac7d |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-10T17:24:29Z |
| publishDate | 2018-10-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-70bef7bae1e04f18a8311620b975ac7d2022-12-22T01:39:53ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502018-10-01331089690310.1590/s0102-865020180100000004Apoptotic mechanisms in rabbits with blast-induced acute lung injuryXiao-Lin QiJian HaoLian-Jiang HuangSheng WuHong-Hao MaZhang-Qing YeHui-Bo HeShu-Wen LiCheng-En LiXin HuangAbstract Purpose: To investigate the apoptotic mechanisms in rabbits with blast-induced acute lung injury (ALI). Methods: A total of 40 rabbits were randomly divided into a blank control group (A, n=10) and an experimental group (EXP, n=30). Explosion-induced chest-ALI models were prepared and sampled at different time points (4, 12, and 24h after modeling, T1-T3) to test the lung dry weight/wet weight ratio (W/D) and arterial oxygen pressure (PaO2), apoptosis of lung tissue by the TUNEL assay, and Caspase-3, Bax, and Bcl-2 levels by immunohistochemical analysis. Furthermore, lung tissue was sampled to observe pathological morphology by microscopy. Results: Under a light microscope, Group EXP exhibited obvious edema in the pulmonary interstitial substance and alveoli, a large number of red blood cells, inflammatory cells, and serous exudation in the alveolar cavity, as well as thickening of the pulmonary interstitial fluid. Compared to Group A, the W/D ratio was significantly increased in Group EXP (P<0.01), while PaO2 was significantly reduced (P<0.01). The apoptosis index was significantly increased (P<0.01), and caspase-3 and Bax/Bcl-2 levels were increased (P<0.01). Conclusion: Apoptosis plays an important role in the occurrence and development of acute lung injury in rabbits by participating in lung injury and promoting the progression of ALI.http://www.scielo.br/pdf/acb/v33n10/1678-2674-acb-33-10-00896.pdfAcute Lung InjuryApoptosisBlast injuriesBlood Gas AnalysisRabbits |
| spellingShingle | Xiao-Lin Qi Jian Hao Lian-Jiang Huang Sheng Wu Hong-Hao Ma Zhang-Qing Ye Hui-Bo He Shu-Wen Li Cheng-En Li Xin Huang Apoptotic mechanisms in rabbits with blast-induced acute lung injury Acta Cirúrgica Brasileira Acute Lung Injury Apoptosis Blast injuries Blood Gas Analysis Rabbits |
| title | Apoptotic mechanisms in rabbits with blast-induced acute lung injury |
| title_full | Apoptotic mechanisms in rabbits with blast-induced acute lung injury |
| title_fullStr | Apoptotic mechanisms in rabbits with blast-induced acute lung injury |
| title_full_unstemmed | Apoptotic mechanisms in rabbits with blast-induced acute lung injury |
| title_short | Apoptotic mechanisms in rabbits with blast-induced acute lung injury |
| title_sort | apoptotic mechanisms in rabbits with blast induced acute lung injury |
| topic | Acute Lung Injury Apoptosis Blast injuries Blood Gas Analysis Rabbits |
| url | http://www.scielo.br/pdf/acb/v33n10/1678-2674-acb-33-10-00896.pdf |
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