Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 gene
Sheeppox virus (SPPV) is considered a highly contagious disease in sheep by the World Organization for Animal Health (OIE). It is classified with Goatpox virus (GTPV) and Lumpy skin disease virus (LSDV) within the Capripoxvirus (CaPV) genus. SPPV causes significant economic losses in endemic regions...
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| Format: | Article |
| Language: | English |
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Institut Agronomique et Vétérinaire Hassan II
2019-06-01
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| Series: | Revue Marocaine des Sciences Agronomiques et Vétérinaires |
| Subjects: | |
| Online Access: | https://www.agrimaroc.org/index.php/Actes_IAVH2/article/view/710/757 |
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| author | Saida HAJJOU Hassan BOULAHYAOUI Khadija KHATABY Chafiqa LOUTFI Malika FAKIRI |
| author_facet | Saida HAJJOU Hassan BOULAHYAOUI Khadija KHATABY Chafiqa LOUTFI Malika FAKIRI |
| author_sort | Saida HAJJOU |
| collection | DOAJ |
| description | Sheeppox virus (SPPV) is considered a highly contagious disease in sheep by the World Organization for Animal Health (OIE). It is classified with Goatpox virus (GTPV) and Lumpy skin disease virus (LSDV) within the Capripoxvirus (CaPV) genus. SPPV causes significant economic losses in endemic regions like Northern and Central Africa, Asia, India and Middle East. In Morocco, little information about the molecular characterization of SPPV is available, hence the objective of the present study is to assess the genomic relationships between Moroccan viral strains isolated from different geographic regions during several outbreaks, vaccine strains and reference strains retrieved from the NCBI Genbank, by sequencing the P32 gene. All sequences were analyzed using MEGA7 software version 7. Phylogenetic tree constructions for this gene sequences were generated using the Neighbor-Joining method. It clearly appeared that SPPV strains reported in many countries, were branched and clustered with the clade of SPPV and displayed a strong genetic relationship between them with nucleotide and amino acid identities respectively of 99-100 % and 98-100 %. These results led us to conclude that P32 gene appears highly conserved among SPPV and Capripoxvirus. For that, more genetic studies are required in order to control and understand the epidemiological situation of SPPV. |
| first_indexed | 2024-12-12T21:39:15Z |
| format | Article |
| id | doaj.art-70bf1cf6d06d43b69d41c3850921092e |
| institution | Directory Open Access Journal |
| issn | 2028-991X 2550-4401 |
| language | English |
| last_indexed | 2024-12-12T21:39:15Z |
| publishDate | 2019-06-01 |
| publisher | Institut Agronomique et Vétérinaire Hassan II |
| record_format | Article |
| series | Revue Marocaine des Sciences Agronomiques et Vétérinaires |
| spelling | doaj.art-70bf1cf6d06d43b69d41c3850921092e2022-12-22T00:11:07ZengInstitut Agronomique et Vétérinaire Hassan IIRevue Marocaine des Sciences Agronomiques et Vétérinaires2028-991X2550-44012019-06-0172252253Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 geneSaida HAJJOU0Hassan BOULAHYAOUI1Khadija KHATABY2Chafiqa LOUTFI3Malika FAKIRI4Université Hassan 1er, Settat, MoroccoFaculty of Medicine & Pharmacy, University Mohamed V, Rabat, MoroccoUniversity Hassan II of Casablanca, MoroccoBiopharma Society, Km 2, Route de Casa, B.P. 4569, Rabat, MoroccoUniversité Hassan 1er, Settat, MoroccoSheeppox virus (SPPV) is considered a highly contagious disease in sheep by the World Organization for Animal Health (OIE). It is classified with Goatpox virus (GTPV) and Lumpy skin disease virus (LSDV) within the Capripoxvirus (CaPV) genus. SPPV causes significant economic losses in endemic regions like Northern and Central Africa, Asia, India and Middle East. In Morocco, little information about the molecular characterization of SPPV is available, hence the objective of the present study is to assess the genomic relationships between Moroccan viral strains isolated from different geographic regions during several outbreaks, vaccine strains and reference strains retrieved from the NCBI Genbank, by sequencing the P32 gene. All sequences were analyzed using MEGA7 software version 7. Phylogenetic tree constructions for this gene sequences were generated using the Neighbor-Joining method. It clearly appeared that SPPV strains reported in many countries, were branched and clustered with the clade of SPPV and displayed a strong genetic relationship between them with nucleotide and amino acid identities respectively of 99-100 % and 98-100 %. These results led us to conclude that P32 gene appears highly conserved among SPPV and Capripoxvirus. For that, more genetic studies are required in order to control and understand the epidemiological situation of SPPV.https://www.agrimaroc.org/index.php/Actes_IAVH2/article/view/710/757Sheeppox virusP32 genePhylogenetic analysisCapripoxvirusGoatpox virus |
| spellingShingle | Saida HAJJOU Hassan BOULAHYAOUI Khadija KHATABY Chafiqa LOUTFI Malika FAKIRI Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 gene Revue Marocaine des Sciences Agronomiques et Vétérinaires Sheeppox virus P32 gene Phylogenetic analysis Capripoxvirus Goatpox virus |
| title | Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 gene |
| title_full | Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 gene |
| title_fullStr | Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 gene |
| title_full_unstemmed | Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 gene |
| title_short | Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 gene |
| title_sort | phylogenetic analysis of moroccan sheeppox virus isolates based on p32 gene |
| topic | Sheeppox virus P32 gene Phylogenetic analysis Capripoxvirus Goatpox virus |
| url | https://www.agrimaroc.org/index.php/Actes_IAVH2/article/view/710/757 |
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