Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo.
Cartilage repair and replacement is a major challenge in plastic reconstructive surgery. The development of a process capable of creating a patient-specific cartilage framework would be a major breakthrough. Here, we described methods for creating human cartilage in vivo and quantitatively assessing...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5728520?pdf=render |
| _version_ | 1818268669682122752 |
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| author | Peter Apelgren Matteo Amoroso Anders Lindahl Camilla Brantsing Nicole Rotter Paul Gatenholm Lars Kölby |
| author_facet | Peter Apelgren Matteo Amoroso Anders Lindahl Camilla Brantsing Nicole Rotter Paul Gatenholm Lars Kölby |
| author_sort | Peter Apelgren |
| collection | DOAJ |
| description | Cartilage repair and replacement is a major challenge in plastic reconstructive surgery. The development of a process capable of creating a patient-specific cartilage framework would be a major breakthrough. Here, we described methods for creating human cartilage in vivo and quantitatively assessing the proliferative capacity and cartilage-formation ability in mono- and co-cultures of human chondrocytes and human mesenchymal stem cells in a three-dimensional (3D)-bioprinted hydrogel scaffold. The 3D-bioprinted constructs (5 × 5 × 1.2 mm) were produced using nanofibrillated cellulose and alginate in combination with human chondrocytes and human mesenchymal stem cells using a 3D-extrusion bioprinter. Immediately following bioprinting, the constructs were implanted subcutaneously on the back of 48 nude mice and explanted after 30 and 60 days, respectively, for morphological and immunohistochemical examination. During explantation, the constructs were easy to handle, and the majority had retained their macroscopic grid appearance. Constructs consisting of human nasal chondrocytes showed good proliferation ability, with 17.2% of the surface areas covered with proliferating chondrocytes after 60 days. In constructs comprising a mixture of chondrocytes and stem cells, an additional proliferative effect was observed involving chondrocyte production of glycosaminoglycans and type 2 collagen. This clinically highly relevant study revealed 3D bioprinting as a promising technology for the creation of human cartilage. |
| first_indexed | 2024-12-12T20:42:10Z |
| format | Article |
| id | doaj.art-70c17f50106d4cfb85a80ebebb43b935 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T20:42:10Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-70c17f50106d4cfb85a80ebebb43b9352022-12-22T00:12:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011212e018942810.1371/journal.pone.0189428Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo.Peter ApelgrenMatteo AmorosoAnders LindahlCamilla BrantsingNicole RotterPaul GatenholmLars KölbyCartilage repair and replacement is a major challenge in plastic reconstructive surgery. The development of a process capable of creating a patient-specific cartilage framework would be a major breakthrough. Here, we described methods for creating human cartilage in vivo and quantitatively assessing the proliferative capacity and cartilage-formation ability in mono- and co-cultures of human chondrocytes and human mesenchymal stem cells in a three-dimensional (3D)-bioprinted hydrogel scaffold. The 3D-bioprinted constructs (5 × 5 × 1.2 mm) were produced using nanofibrillated cellulose and alginate in combination with human chondrocytes and human mesenchymal stem cells using a 3D-extrusion bioprinter. Immediately following bioprinting, the constructs were implanted subcutaneously on the back of 48 nude mice and explanted after 30 and 60 days, respectively, for morphological and immunohistochemical examination. During explantation, the constructs were easy to handle, and the majority had retained their macroscopic grid appearance. Constructs consisting of human nasal chondrocytes showed good proliferation ability, with 17.2% of the surface areas covered with proliferating chondrocytes after 60 days. In constructs comprising a mixture of chondrocytes and stem cells, an additional proliferative effect was observed involving chondrocyte production of glycosaminoglycans and type 2 collagen. This clinically highly relevant study revealed 3D bioprinting as a promising technology for the creation of human cartilage.http://europepmc.org/articles/PMC5728520?pdf=render |
| spellingShingle | Peter Apelgren Matteo Amoroso Anders Lindahl Camilla Brantsing Nicole Rotter Paul Gatenholm Lars Kölby Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo. PLoS ONE |
| title | Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo. |
| title_full | Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo. |
| title_fullStr | Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo. |
| title_full_unstemmed | Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo. |
| title_short | Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo. |
| title_sort | chondrocytes and stem cells in 3d bioprinted structures create human cartilage in vivo |
| url | http://europepmc.org/articles/PMC5728520?pdf=render |
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