Novel Fluorescent Probe Based on Rare-Earth Doped Upconversion Nanomaterials and Its Applications in Early Cancer Detection
In this paper, a novel rare-earth-doped upconverted nanomaterial NaYF<sub>4</sub>:Yb,Tm fluorescent probe is reported, which can detect cancer-related specific miRNAs in low abundance. The detection is based on an upconversion of nanomaterials NaYF<sub>4</sub>:Yb,Tm, with emi...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/11/1787 |
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| author | Zhou Ding Yue He Hongtao Rao Le Zhang William Nguyen Jingjing Wang Ying Wu Caiqin Han Christina Xing Changchun Yan Wei Chen Ying Liu |
| author_facet | Zhou Ding Yue He Hongtao Rao Le Zhang William Nguyen Jingjing Wang Ying Wu Caiqin Han Christina Xing Changchun Yan Wei Chen Ying Liu |
| author_sort | Zhou Ding |
| collection | DOAJ |
| description | In this paper, a novel rare-earth-doped upconverted nanomaterial NaYF<sub>4</sub>:Yb,Tm fluorescent probe is reported, which can detect cancer-related specific miRNAs in low abundance. The detection is based on an upconversion of nanomaterials NaYF<sub>4</sub>:Yb,Tm, with emissions at 345, 362, 450, 477, 646, and 802 nm, upon excitation at 980 nm. The optimal Yb<sup>3+</sup>:Tm<sup>3+</sup> doping ratio is 40:1, in which the NaYF<sub>4</sub>:Yb,Tm nanomaterials have the strongest fluorescence. The NaYF<sub>4</sub>:Yb, Tm nanoparticles were coated with carboxylation or carboxylated protein, in order to improve their water solubility and biocompatibility. The two commonly expressed proteins, miRNA-155 and miRNA-150, were detected by the designed fluorescent probe. The results showed that the probes can distinguish miRNA-155 well from partial and complete base mismatch miRNA-155, and can effectively distinguish miRNA-155 and miRNA-150. The preliminary results indicate that these upconverted nanomaterials have good potential for protein detection in disease diagnosis, including early cancer detection. |
| first_indexed | 2024-03-10T01:02:37Z |
| format | Article |
| id | doaj.art-70c264130b8741b3900d18f7cc2733ec |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T01:02:37Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-70c264130b8741b3900d18f7cc2733ec2023-11-23T14:32:18ZengMDPI AGNanomaterials2079-49912022-05-011211178710.3390/nano12111787Novel Fluorescent Probe Based on Rare-Earth Doped Upconversion Nanomaterials and Its Applications in Early Cancer DetectionZhou Ding0Yue He1Hongtao Rao2Le Zhang3William Nguyen4Jingjing Wang5Ying Wu6Caiqin Han7Christina Xing8Changchun Yan9Wei Chen10Ying Liu11Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaJiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaJiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaJiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaDepartment of Physics, The University of Texas at Arlington, Arlington, TX 76019-0059, USAJiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaJiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaJiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaDepartment of Physics, The University of Texas at Arlington, Arlington, TX 76019-0059, USAJiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaDepartment of Physics, The University of Texas at Arlington, Arlington, TX 76019-0059, USAJiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, ChinaIn this paper, a novel rare-earth-doped upconverted nanomaterial NaYF<sub>4</sub>:Yb,Tm fluorescent probe is reported, which can detect cancer-related specific miRNAs in low abundance. The detection is based on an upconversion of nanomaterials NaYF<sub>4</sub>:Yb,Tm, with emissions at 345, 362, 450, 477, 646, and 802 nm, upon excitation at 980 nm. The optimal Yb<sup>3+</sup>:Tm<sup>3+</sup> doping ratio is 40:1, in which the NaYF<sub>4</sub>:Yb,Tm nanomaterials have the strongest fluorescence. The NaYF<sub>4</sub>:Yb, Tm nanoparticles were coated with carboxylation or carboxylated protein, in order to improve their water solubility and biocompatibility. The two commonly expressed proteins, miRNA-155 and miRNA-150, were detected by the designed fluorescent probe. The results showed that the probes can distinguish miRNA-155 well from partial and complete base mismatch miRNA-155, and can effectively distinguish miRNA-155 and miRNA-150. The preliminary results indicate that these upconverted nanomaterials have good potential for protein detection in disease diagnosis, including early cancer detection.https://www.mdpi.com/2079-4991/12/11/1787upconversion nanomaterialsnovel fluorescent probemiRNA-155miRNA-150 |
| spellingShingle | Zhou Ding Yue He Hongtao Rao Le Zhang William Nguyen Jingjing Wang Ying Wu Caiqin Han Christina Xing Changchun Yan Wei Chen Ying Liu Novel Fluorescent Probe Based on Rare-Earth Doped Upconversion Nanomaterials and Its Applications in Early Cancer Detection Nanomaterials upconversion nanomaterials novel fluorescent probe miRNA-155 miRNA-150 |
| title | Novel Fluorescent Probe Based on Rare-Earth Doped Upconversion Nanomaterials and Its Applications in Early Cancer Detection |
| title_full | Novel Fluorescent Probe Based on Rare-Earth Doped Upconversion Nanomaterials and Its Applications in Early Cancer Detection |
| title_fullStr | Novel Fluorescent Probe Based on Rare-Earth Doped Upconversion Nanomaterials and Its Applications in Early Cancer Detection |
| title_full_unstemmed | Novel Fluorescent Probe Based on Rare-Earth Doped Upconversion Nanomaterials and Its Applications in Early Cancer Detection |
| title_short | Novel Fluorescent Probe Based on Rare-Earth Doped Upconversion Nanomaterials and Its Applications in Early Cancer Detection |
| title_sort | novel fluorescent probe based on rare earth doped upconversion nanomaterials and its applications in early cancer detection |
| topic | upconversion nanomaterials novel fluorescent probe miRNA-155 miRNA-150 |
| url | https://www.mdpi.com/2079-4991/12/11/1787 |
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