ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE
The ageing process is influenced by many internal and external factors. The toxic substances in the environment can cause genomic damages to cells, which increase the risk of early ageing. Furthermore, the cytochrome P450 1A2 (CYP1A2) gene polymorphism is a susceptibility factor and may enhance the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2013-12-01
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| Series: | Balkan Journal of Medical Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/bjmg-2013-0031 |
| _version_ | 1797761240849711104 |
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| author | Eshkoor Sa. Ismail P. Rahman Sa. Moin S. Adon My. |
| author_facet | Eshkoor Sa. Ismail P. Rahman Sa. Moin S. Adon My. |
| author_sort | Eshkoor Sa. |
| collection | DOAJ |
| description | The ageing process is influenced by many internal and external factors. The toxic substances in the environment can cause genomic damages to cells, which increase the risk of early ageing. Furthermore, the cytochrome P450 1A2 (CYP1A2) gene polymorphism is a susceptibility factor and may enhance the risk of DNA damage in cells. The current study was carried out to show whether occupational exposure could cause genotoxicity in cells carrying the CYP1A2 gene polymorphism, thus enhancing the likelihood of early ageing. This study was conducted on mechanical workshop workers and a control group by collecting buccal cells from their mouths. Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was used to identify the CYP1A2 gene polymorphism in the cells. In addition, three extra methods including micronuclei (MN) test, comet assay and real-time PCR (RT-PCR) were applied to determine the effects of gene polymorphisms on DNA damage and ageing from occupational exposure. The results showed that DNA damage in the cells carrying the mutated genotype was higher than the wild genotype. In addition, the difference in MN frequency (p = 0.001) and relative telomere length (p = 0.002) between workers and controls was significant (p <0.05) in the mutated genotype. The findings indicated a possible protective effect of gene polymorphism against early ageing, which was characterized by lack of a significant influence of CYP1A2 gene polymorphism on genetic material in the subjects (p >0.05). It was concluded that the CYP1A2 gene could be a contributing factor to prevent early ageing from occupational exposure. |
| first_indexed | 2024-03-12T19:10:07Z |
| format | Article |
| id | doaj.art-70c2eda56db34b9ebd33cb55b069a6e7 |
| institution | Directory Open Access Journal |
| issn | 1311-0160 |
| language | English |
| last_indexed | 2024-03-12T19:10:07Z |
| publishDate | 2013-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Balkan Journal of Medical Genetics |
| spelling | doaj.art-70c2eda56db34b9ebd33cb55b069a6e72023-08-02T05:57:07ZengSciendoBalkan Journal of Medical Genetics1311-01602013-12-01162455210.2478/bjmg-2013-0031ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSUREEshkoor Sa.0Ismail P.1Rahman Sa.2Moin S.3Adon My.4Institute of Gerontology, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang, Selangor, MalaysiaInstitute of Gerontology, Universiti Putra Malaysia (UPM), Serdang, Selangor, MalaysiaDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang, Selangor, MalaysiaDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang, Selangor, MalaysiaInstitute for Medical Research, Ministry of Health Malaysia, Kuala Lumpur, MalaysiaThe ageing process is influenced by many internal and external factors. The toxic substances in the environment can cause genomic damages to cells, which increase the risk of early ageing. Furthermore, the cytochrome P450 1A2 (CYP1A2) gene polymorphism is a susceptibility factor and may enhance the risk of DNA damage in cells. The current study was carried out to show whether occupational exposure could cause genotoxicity in cells carrying the CYP1A2 gene polymorphism, thus enhancing the likelihood of early ageing. This study was conducted on mechanical workshop workers and a control group by collecting buccal cells from their mouths. Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was used to identify the CYP1A2 gene polymorphism in the cells. In addition, three extra methods including micronuclei (MN) test, comet assay and real-time PCR (RT-PCR) were applied to determine the effects of gene polymorphisms on DNA damage and ageing from occupational exposure. The results showed that DNA damage in the cells carrying the mutated genotype was higher than the wild genotype. In addition, the difference in MN frequency (p = 0.001) and relative telomere length (p = 0.002) between workers and controls was significant (p <0.05) in the mutated genotype. The findings indicated a possible protective effect of gene polymorphism against early ageing, which was characterized by lack of a significant influence of CYP1A2 gene polymorphism on genetic material in the subjects (p >0.05). It was concluded that the CYP1A2 gene could be a contributing factor to prevent early ageing from occupational exposure.https://doi.org/10.2478/bjmg-2013-0031ageingbuccal cellscytochrome p450 (cyp)cytochrome p450 1a2 (cyp1a2) gene polymorphismmicronuclei (mn) testoccupational exposure |
| spellingShingle | Eshkoor Sa. Ismail P. Rahman Sa. Moin S. Adon My. ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE Balkan Journal of Medical Genetics ageing buccal cells cytochrome p450 (cyp) cytochrome p450 1a2 (cyp1a2) gene polymorphism micronuclei (mn) test occupational exposure |
| title | ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE |
| title_full | ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE |
| title_fullStr | ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE |
| title_full_unstemmed | ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE |
| title_short | ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE |
| title_sort | role of the cyp1a2 gene polymorphism on early ageing from occupational exposure |
| topic | ageing buccal cells cytochrome p450 (cyp) cytochrome p450 1a2 (cyp1a2) gene polymorphism micronuclei (mn) test occupational exposure |
| url | https://doi.org/10.2478/bjmg-2013-0031 |
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