Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis
BackgroundThe status of the sentinel lymph nodes (SLNs) was an important prognostic factor in varies cancers. A one-step nucleic acid amplification (OSNA) assay, a molecular-based whole-node analysis method based on CK19 mRNA copy number, was developed to diagnose lymph node metastases. We aimed to...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-04-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1370709/full |
| _version_ | 1797219322111721472 |
|---|---|
| author | Ke Li Min Meng Weiwei Zhang Junyi Li Yiting Wang Changhui Zhou |
| author_facet | Ke Li Min Meng Weiwei Zhang Junyi Li Yiting Wang Changhui Zhou |
| author_sort | Ke Li |
| collection | DOAJ |
| description | BackgroundThe status of the sentinel lymph nodes (SLNs) was an important prognostic factor in varies cancers. A one-step nucleic acid amplification (OSNA) assay, a molecular-based whole-node analysis method based on CK19 mRNA copy number, was developed to diagnose lymph node metastases. We aimed to evaluate the value of OSNA for the diagnosis of sentinel lymph node metastasis in CK19 positive cancers. CK19 mRNA and protein expression for pan-caner analysis were obtained from TCGA and the Human protein atlas database.MethodsTwo researchers independently searched the PubMed, Cochrane Library and Web of Science databases for qualified articles published before December 1, 2023. A meta-analysis was performed using MetaDisc and STATA. Risk bias and quality assessments of the included studies were evaluated, and a subgroup analysis was performed. Ten cancer types were found to be CK19 positively expressed and 7 of 10 had been reported to use OSNA for SLN detection.ResultsAfter literature review, there were 61 articles included in the meta-analysis, which consisted of 7115 patients with 18007 sentinel lymph nodes. The pooled sensitivity and specificity of OSNA were 0.87 and 0.95 in overall patients. Moreover, we found the background CK19 expression in normal tissue affected the diagnostic accuracy of OSNA. In breast cancer, we performed subgroup analysis. OSNA exhibited to be a stable method across different population groups and various medical centers. In addition, when 250 copies/μl was chosen as the cutoff point of CK19 mRNA, there were a relatively higher sensitivity and AUC in detecting SLN micro-metastasis than 5000 copies/μl.DiscussionOSNA can predict the occurrence of SLN metastasis accurately in CK19 positive cancers, especially in breast cancer, colorectal cancer, lung cancer, gastric cancer and endometrial cancer. Our study warrants future studies investigating the clinical application of OSNA in pancreatic, ovarian and bladder cancers. |
| first_indexed | 2024-04-24T12:31:48Z |
| format | Article |
| id | doaj.art-70c3d2f07e2f407c836a4fa352099b6f |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-24T12:31:48Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-70c3d2f07e2f407c836a4fa352099b6f2024-04-08T04:27:38ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2024-04-011410.3389/fonc.2024.13707091370709Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysisKe Li0Min Meng1Weiwei Zhang2Junyi Li3Yiting Wang4Changhui Zhou5Department of Central Laboratory, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaDepartment of Central Laboratory, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaDepartment of Central Laboratory, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaDepartment of Clinical Medicine, Shandong First Medical University, Jinan, Shandong, ChinaDepartment of Central Laboratory, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaDepartment of Central Laboratory, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaBackgroundThe status of the sentinel lymph nodes (SLNs) was an important prognostic factor in varies cancers. A one-step nucleic acid amplification (OSNA) assay, a molecular-based whole-node analysis method based on CK19 mRNA copy number, was developed to diagnose lymph node metastases. We aimed to evaluate the value of OSNA for the diagnosis of sentinel lymph node metastasis in CK19 positive cancers. CK19 mRNA and protein expression for pan-caner analysis were obtained from TCGA and the Human protein atlas database.MethodsTwo researchers independently searched the PubMed, Cochrane Library and Web of Science databases for qualified articles published before December 1, 2023. A meta-analysis was performed using MetaDisc and STATA. Risk bias and quality assessments of the included studies were evaluated, and a subgroup analysis was performed. Ten cancer types were found to be CK19 positively expressed and 7 of 10 had been reported to use OSNA for SLN detection.ResultsAfter literature review, there were 61 articles included in the meta-analysis, which consisted of 7115 patients with 18007 sentinel lymph nodes. The pooled sensitivity and specificity of OSNA were 0.87 and 0.95 in overall patients. Moreover, we found the background CK19 expression in normal tissue affected the diagnostic accuracy of OSNA. In breast cancer, we performed subgroup analysis. OSNA exhibited to be a stable method across different population groups and various medical centers. In addition, when 250 copies/μl was chosen as the cutoff point of CK19 mRNA, there were a relatively higher sensitivity and AUC in detecting SLN micro-metastasis than 5000 copies/μl.DiscussionOSNA can predict the occurrence of SLN metastasis accurately in CK19 positive cancers, especially in breast cancer, colorectal cancer, lung cancer, gastric cancer and endometrial cancer. Our study warrants future studies investigating the clinical application of OSNA in pancreatic, ovarian and bladder cancers.https://www.frontiersin.org/articles/10.3389/fonc.2024.1370709/fullOSNACK19sentinel lymph nodesmetastasismeta-analysis |
| spellingShingle | Ke Li Min Meng Weiwei Zhang Junyi Li Yiting Wang Changhui Zhou Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis Frontiers in Oncology OSNA CK19 sentinel lymph nodes metastasis meta-analysis |
| title | Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis |
| title_full | Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis |
| title_fullStr | Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis |
| title_full_unstemmed | Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis |
| title_short | Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis |
| title_sort | diagnostic value of one step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19 positive tumors evidence from bioinformatics and meta analysis |
| topic | OSNA CK19 sentinel lymph nodes metastasis meta-analysis |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1370709/full |
| work_keys_str_mv | AT keli diagnosticvalueofonestepnucleicacidamplificationforsentinellymphnodemetastasisincytokeratin19positivetumorsevidencefrombioinformaticsandmetaanalysis AT minmeng diagnosticvalueofonestepnucleicacidamplificationforsentinellymphnodemetastasisincytokeratin19positivetumorsevidencefrombioinformaticsandmetaanalysis AT weiweizhang diagnosticvalueofonestepnucleicacidamplificationforsentinellymphnodemetastasisincytokeratin19positivetumorsevidencefrombioinformaticsandmetaanalysis AT junyili diagnosticvalueofonestepnucleicacidamplificationforsentinellymphnodemetastasisincytokeratin19positivetumorsevidencefrombioinformaticsandmetaanalysis AT yitingwang diagnosticvalueofonestepnucleicacidamplificationforsentinellymphnodemetastasisincytokeratin19positivetumorsevidencefrombioinformaticsandmetaanalysis AT changhuizhou diagnosticvalueofonestepnucleicacidamplificationforsentinellymphnodemetastasisincytokeratin19positivetumorsevidencefrombioinformaticsandmetaanalysis |