Partial Analysis 16S rRNA Gene in Lactobacillus spp. from Natural Fermented Milk
Purpose: The aim of this research is to analysis 16S rRNA gene sequence of Lactobacillus spp. from natural fermented milk. Material and Methods: The methods that used in this study are total DNA isolation using alkaline lysis, PCR amplification carried out using of specific primers, and identificat...
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| Format: | Article |
| Language: | English |
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Cukurova University
2014-02-01
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| Series: | Çukurova Üniversitesi Tıp Fakültesi Dergisi |
| Subjects: | |
| Online Access: | http://www.scopemed.org/fulltextpdf.php?mno=43829 |
| _version_ | 1828023065756303360 |
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| author | Nur Kusmiyati Nur Hidayat Fatchiyah Fatchiyah |
| author_facet | Nur Kusmiyati Nur Hidayat Fatchiyah Fatchiyah |
| author_sort | Nur Kusmiyati |
| collection | DOAJ |
| description | Purpose: The aim of this research is to analysis 16S rRNA gene sequence of Lactobacillus spp. from natural fermented milk.
Material and Methods: The methods that used in this study are total DNA isolation using alkaline lysis, PCR amplification carried out using of specific primers, and identification of amplified DNA using sequencing. The result of PCR process is a nuclotide with approximately 585 bp length which contained V1-V3 variable regions. 16S rRNA gene sequence then compared and aligned with existing 16S rRNA data sequence from GenBank.
Results: The conserved region of 16S rRNA gene from nuclotide number 189-358 and 384-445 were 95,9% and 94,9% respectively, and the total conserved region of 16S rRNA gene is 78.8%. From total 7 isolates that isolated from natural fermented milk , 2 isolates were identified as L. plantarum with similarity value 98.28% and 97.78%, the other 5 isolates were identified as L. rhamnosus (2 isolates) with similarity value 98.38% and 97.51%, L. zeae (2 isolates) with similarity value 97.36% and 98.38%, and L. casei (1 isolate) with similarity value 98.20%.
Conclusion: These results indicate the test isolates are members of the genus Lactobacillus, but classified in different species |
| first_indexed | 2024-04-10T12:20:10Z |
| format | Article |
| id | doaj.art-70c3dd243aa84fe4ab0558c936649e5d |
| institution | Directory Open Access Journal |
| issn | 0250-5150 |
| language | English |
| last_indexed | 2024-04-10T12:20:10Z |
| publishDate | 2014-02-01 |
| publisher | Cukurova University |
| record_format | Article |
| series | Çukurova Üniversitesi Tıp Fakültesi Dergisi |
| spelling | doaj.art-70c3dd243aa84fe4ab0558c936649e5d2023-02-15T16:15:29ZengCukurova UniversityÇukurova Üniversitesi Tıp Fakültesi Dergisi0250-51502014-02-013919910443829Partial Analysis 16S rRNA Gene in Lactobacillus spp. from Natural Fermented MilkNur Kusmiyati0Nur Hidayat1Fatchiyah Fatchiyah2Brawijaya University Brawijaya University Brawijaya UniversityPurpose: The aim of this research is to analysis 16S rRNA gene sequence of Lactobacillus spp. from natural fermented milk. Material and Methods: The methods that used in this study are total DNA isolation using alkaline lysis, PCR amplification carried out using of specific primers, and identification of amplified DNA using sequencing. The result of PCR process is a nuclotide with approximately 585 bp length which contained V1-V3 variable regions. 16S rRNA gene sequence then compared and aligned with existing 16S rRNA data sequence from GenBank. Results: The conserved region of 16S rRNA gene from nuclotide number 189-358 and 384-445 were 95,9% and 94,9% respectively, and the total conserved region of 16S rRNA gene is 78.8%. From total 7 isolates that isolated from natural fermented milk , 2 isolates were identified as L. plantarum with similarity value 98.28% and 97.78%, the other 5 isolates were identified as L. rhamnosus (2 isolates) with similarity value 98.38% and 97.51%, L. zeae (2 isolates) with similarity value 97.36% and 98.38%, and L. casei (1 isolate) with similarity value 98.20%. Conclusion: These results indicate the test isolates are members of the genus Lactobacillus, but classified in different specieshttp://www.scopemed.org/fulltextpdf.php?mno=4382916S rRNA geneLactobacillusnatural fermentation milkpartial analysis |
| spellingShingle | Nur Kusmiyati Nur Hidayat Fatchiyah Fatchiyah Partial Analysis 16S rRNA Gene in Lactobacillus spp. from Natural Fermented Milk Çukurova Üniversitesi Tıp Fakültesi Dergisi 16S rRNA gene Lactobacillus natural fermentation milk partial analysis |
| title | Partial Analysis 16S rRNA Gene in Lactobacillus spp. from Natural Fermented Milk |
| title_full | Partial Analysis 16S rRNA Gene in Lactobacillus spp. from Natural Fermented Milk |
| title_fullStr | Partial Analysis 16S rRNA Gene in Lactobacillus spp. from Natural Fermented Milk |
| title_full_unstemmed | Partial Analysis 16S rRNA Gene in Lactobacillus spp. from Natural Fermented Milk |
| title_short | Partial Analysis 16S rRNA Gene in Lactobacillus spp. from Natural Fermented Milk |
| title_sort | partial analysis 16s rrna gene in lactobacillus spp from natural fermented milk |
| topic | 16S rRNA gene Lactobacillus natural fermentation milk partial analysis |
| url | http://www.scopemed.org/fulltextpdf.php?mno=43829 |
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