An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studies
To date, there has been no ideal method for blood platelet isolation which allows one to obtain a preparation devoid of contaminations, reflecting the activation status and morphological features of circulating platelets. To address these requirements, we have developed a method which combines the c...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2016.1209478 |
| _version_ | 1827816991738560512 |
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| author | Aneta Wrzyszcz Joanna Urbaniak Agnieszka Sapa Mieczysław Woźniak |
| author_facet | Aneta Wrzyszcz Joanna Urbaniak Agnieszka Sapa Mieczysław Woźniak |
| author_sort | Aneta Wrzyszcz |
| collection | DOAJ |
| description | To date, there has been no ideal method for blood platelet isolation which allows one to obtain a preparation devoid of contaminations, reflecting the activation status and morphological features of circulating platelets. To address these requirements, we have developed a method which combines the continuous density gradient centrifugation with washing from PGI2-supplemented platelet-rich plasma (PRP). We have assessed the degree of erythrocyte and leukocyte contamination, recovery of platelets, morphological features, activation status, and reactivity of isolated platelets. Using our protocol, we were able to get a preparation free from contaminations, representing well the platelet population prior to the isolation in terms of size and activity. Besides this, we have obtained approximately 2 times more platelets from the same volume of blood compared to the most widely used method. From 10 ml of whole citrated blood we were able to get on average 2.7 mg of platelet-derived protein. The method of platelet isolation presented in this paper can be successfully applied to tests requiring very pure platelets, reflecting the circulating platelet state, from a small volume of blood. |
| first_indexed | 2024-03-12T00:28:09Z |
| format | Article |
| id | doaj.art-70c450e1e9e94f6a85db8e3941cb8f4a |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:28:09Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-70c450e1e9e94f6a85db8e3941cb8f4a2023-09-15T10:26:28ZengTaylor & Francis GroupPlatelets0953-71041369-16352017-01-01281435310.1080/09537104.2016.12094781209478An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studiesAneta Wrzyszcz0Joanna Urbaniak1Agnieszka Sapa2Mieczysław Woźniak3Wroclaw Medical UniversityLower Silesian Oncology CenterWroclaw Medical UniversityWroclaw Medical UniversityTo date, there has been no ideal method for blood platelet isolation which allows one to obtain a preparation devoid of contaminations, reflecting the activation status and morphological features of circulating platelets. To address these requirements, we have developed a method which combines the continuous density gradient centrifugation with washing from PGI2-supplemented platelet-rich plasma (PRP). We have assessed the degree of erythrocyte and leukocyte contamination, recovery of platelets, morphological features, activation status, and reactivity of isolated platelets. Using our protocol, we were able to get a preparation free from contaminations, representing well the platelet population prior to the isolation in terms of size and activity. Besides this, we have obtained approximately 2 times more platelets from the same volume of blood compared to the most widely used method. From 10 ml of whole citrated blood we were able to get on average 2.7 mg of platelet-derived protein. The method of platelet isolation presented in this paper can be successfully applied to tests requiring very pure platelets, reflecting the circulating platelet state, from a small volume of blood.http://dx.doi.org/10.1080/09537104.2016.1209478density gradient centrifugationplatelet isolationplatelet proteomicsplatelets |
| spellingShingle | Aneta Wrzyszcz Joanna Urbaniak Agnieszka Sapa Mieczysław Woźniak An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studies Platelets density gradient centrifugation platelet isolation platelet proteomics platelets |
| title | An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studies |
| title_full | An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studies |
| title_fullStr | An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studies |
| title_full_unstemmed | An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studies |
| title_short | An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studies |
| title_sort | efficient method for isolation of representative and contamination free population of blood platelets for proteomic studies |
| topic | density gradient centrifugation platelet isolation platelet proteomics platelets |
| url | http://dx.doi.org/10.1080/09537104.2016.1209478 |
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