Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.

Transcription in mammalian nuclei is highly compartmentalized in RNA polymerase II-enriched nuclear foci known as transcription factories. Genes in cis and trans can share the same factory, suggesting that genes migrate to preassembled transcription sites. We used fluorescent in situ hybridization t...

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Main Authors: Cameron S Osborne, Lyubomira Chakalova, Jennifer A Mitchell, Alice Horton, Andrew L Wood, Daniel J Bolland, Anne E Corcoran, Peter Fraser
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2007-08-01
Series:PLoS Biology
Online Access:https://doi.org/10.1371/journal.pbio.0050192
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author Cameron S Osborne
Lyubomira Chakalova
Jennifer A Mitchell
Alice Horton
Andrew L Wood
Daniel J Bolland
Anne E Corcoran
Peter Fraser
author_facet Cameron S Osborne
Lyubomira Chakalova
Jennifer A Mitchell
Alice Horton
Andrew L Wood
Daniel J Bolland
Anne E Corcoran
Peter Fraser
author_sort Cameron S Osborne
collection DOAJ
description Transcription in mammalian nuclei is highly compartmentalized in RNA polymerase II-enriched nuclear foci known as transcription factories. Genes in cis and trans can share the same factory, suggesting that genes migrate to preassembled transcription sites. We used fluorescent in situ hybridization to investigate the dynamics of gene association with transcription factories during immediate early (IE) gene induction in mouse B lymphocytes. Here, we show that induction involves rapid gene relocation to transcription factories. Importantly, we find that the Myc proto-oncogene on Chromosome 15 is preferentially recruited to the same transcription factory as the highly transcribed Igh gene located on Chromosome 12. Myc and Igh are the most frequent translocation partners in plasmacytoma and Burkitt lymphoma. Our results show that transcriptional activation of IE genes involves rapid relocation to preassembled transcription factories. Furthermore, the data imply a direct link between the nonrandom interchromosomal organization of transcribed genes at transcription factories and the incidence of specific chromosomal translocations.
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spelling doaj.art-70c4679520954fabb6c965ff8f31b5152022-12-22T04:10:04ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852007-08-0158e19210.1371/journal.pbio.0050192Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.Cameron S OsborneLyubomira ChakalovaJennifer A MitchellAlice HortonAndrew L WoodDaniel J BollandAnne E CorcoranPeter FraserTranscription in mammalian nuclei is highly compartmentalized in RNA polymerase II-enriched nuclear foci known as transcription factories. Genes in cis and trans can share the same factory, suggesting that genes migrate to preassembled transcription sites. We used fluorescent in situ hybridization to investigate the dynamics of gene association with transcription factories during immediate early (IE) gene induction in mouse B lymphocytes. Here, we show that induction involves rapid gene relocation to transcription factories. Importantly, we find that the Myc proto-oncogene on Chromosome 15 is preferentially recruited to the same transcription factory as the highly transcribed Igh gene located on Chromosome 12. Myc and Igh are the most frequent translocation partners in plasmacytoma and Burkitt lymphoma. Our results show that transcriptional activation of IE genes involves rapid relocation to preassembled transcription factories. Furthermore, the data imply a direct link between the nonrandom interchromosomal organization of transcribed genes at transcription factories and the incidence of specific chromosomal translocations.https://doi.org/10.1371/journal.pbio.0050192
spellingShingle Cameron S Osborne
Lyubomira Chakalova
Jennifer A Mitchell
Alice Horton
Andrew L Wood
Daniel J Bolland
Anne E Corcoran
Peter Fraser
Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
PLoS Biology
title Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
title_full Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
title_fullStr Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
title_full_unstemmed Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
title_short Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
title_sort myc dynamically and preferentially relocates to a transcription factory occupied by igh
url https://doi.org/10.1371/journal.pbio.0050192
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