Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
Transcription in mammalian nuclei is highly compartmentalized in RNA polymerase II-enriched nuclear foci known as transcription factories. Genes in cis and trans can share the same factory, suggesting that genes migrate to preassembled transcription sites. We used fluorescent in situ hybridization t...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2007-08-01
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Series: | PLoS Biology |
Online Access: | https://doi.org/10.1371/journal.pbio.0050192 |
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author | Cameron S Osborne Lyubomira Chakalova Jennifer A Mitchell Alice Horton Andrew L Wood Daniel J Bolland Anne E Corcoran Peter Fraser |
author_facet | Cameron S Osborne Lyubomira Chakalova Jennifer A Mitchell Alice Horton Andrew L Wood Daniel J Bolland Anne E Corcoran Peter Fraser |
author_sort | Cameron S Osborne |
collection | DOAJ |
description | Transcription in mammalian nuclei is highly compartmentalized in RNA polymerase II-enriched nuclear foci known as transcription factories. Genes in cis and trans can share the same factory, suggesting that genes migrate to preassembled transcription sites. We used fluorescent in situ hybridization to investigate the dynamics of gene association with transcription factories during immediate early (IE) gene induction in mouse B lymphocytes. Here, we show that induction involves rapid gene relocation to transcription factories. Importantly, we find that the Myc proto-oncogene on Chromosome 15 is preferentially recruited to the same transcription factory as the highly transcribed Igh gene located on Chromosome 12. Myc and Igh are the most frequent translocation partners in plasmacytoma and Burkitt lymphoma. Our results show that transcriptional activation of IE genes involves rapid relocation to preassembled transcription factories. Furthermore, the data imply a direct link between the nonrandom interchromosomal organization of transcribed genes at transcription factories and the incidence of specific chromosomal translocations. |
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format | Article |
id | doaj.art-70c4679520954fabb6c965ff8f31b515 |
institution | Directory Open Access Journal |
issn | 1544-9173 1545-7885 |
language | English |
last_indexed | 2024-04-11T18:11:56Z |
publishDate | 2007-08-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS Biology |
spelling | doaj.art-70c4679520954fabb6c965ff8f31b5152022-12-22T04:10:04ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852007-08-0158e19210.1371/journal.pbio.0050192Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.Cameron S OsborneLyubomira ChakalovaJennifer A MitchellAlice HortonAndrew L WoodDaniel J BollandAnne E CorcoranPeter FraserTranscription in mammalian nuclei is highly compartmentalized in RNA polymerase II-enriched nuclear foci known as transcription factories. Genes in cis and trans can share the same factory, suggesting that genes migrate to preassembled transcription sites. We used fluorescent in situ hybridization to investigate the dynamics of gene association with transcription factories during immediate early (IE) gene induction in mouse B lymphocytes. Here, we show that induction involves rapid gene relocation to transcription factories. Importantly, we find that the Myc proto-oncogene on Chromosome 15 is preferentially recruited to the same transcription factory as the highly transcribed Igh gene located on Chromosome 12. Myc and Igh are the most frequent translocation partners in plasmacytoma and Burkitt lymphoma. Our results show that transcriptional activation of IE genes involves rapid relocation to preassembled transcription factories. Furthermore, the data imply a direct link between the nonrandom interchromosomal organization of transcribed genes at transcription factories and the incidence of specific chromosomal translocations.https://doi.org/10.1371/journal.pbio.0050192 |
spellingShingle | Cameron S Osborne Lyubomira Chakalova Jennifer A Mitchell Alice Horton Andrew L Wood Daniel J Bolland Anne E Corcoran Peter Fraser Myc dynamically and preferentially relocates to a transcription factory occupied by Igh. PLoS Biology |
title | Myc dynamically and preferentially relocates to a transcription factory occupied by Igh. |
title_full | Myc dynamically and preferentially relocates to a transcription factory occupied by Igh. |
title_fullStr | Myc dynamically and preferentially relocates to a transcription factory occupied by Igh. |
title_full_unstemmed | Myc dynamically and preferentially relocates to a transcription factory occupied by Igh. |
title_short | Myc dynamically and preferentially relocates to a transcription factory occupied by Igh. |
title_sort | myc dynamically and preferentially relocates to a transcription factory occupied by igh |
url | https://doi.org/10.1371/journal.pbio.0050192 |
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