Promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol-oxidase-mediated crosslinking of interfacial protein in emulsions
Previously, polyunsaturated fatty acids (PUFA) from linseed oil were effectively protected (>80%) against biohydrogenation through polyphenol-oxidase-mediated protein crosslinking of an emulsion, prepared with polyphenol oxidase (PPO) extract from potato tuber peelings. However, until now, emulsi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Animal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731118000423 |
| _version_ | 1818928744629272576 |
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| author | N. De Neve B. Vlaeminck F. Gadeyne E. Claeys P. Van der Meeren V. Fievez |
| author_facet | N. De Neve B. Vlaeminck F. Gadeyne E. Claeys P. Van der Meeren V. Fievez |
| author_sort | N. De Neve |
| collection | DOAJ |
| description | Previously, polyunsaturated fatty acids (PUFA) from linseed oil were effectively protected (>80%) against biohydrogenation through polyphenol-oxidase-mediated protein crosslinking of an emulsion, prepared with polyphenol oxidase (PPO) extract from potato tuber peelings. However, until now, emulsions of only 2 wt% oil have been successfully protected, which implies serious limitations both from a research perspective (e.g. in vivo trials) as well as for further upscaling toward practical applications. Therefore, the aim of this study was to increase the oil/PPO ratio. In the original protocol, the PPO extract served both an emulsifying function as well as a crosslinking function. Here, it was first evaluated whether alternative protein sources could replace the emulsifying function of the PPO extract, with addition of PPO extract and 4-methylcatechol (4MC) to induce crosslinking after emulsion preparation. This approach was then further used to evaluate protection of emulsions with higher oil content. Five candidate emulsifiers (soy glycinin, gelatin, whey protein isolate (WPI), bovine serum albumin and sodium caseinate) were used to prepare 10 wt% oil emulsions, which were diluted five times (w/w) with PPO extract (experiment 1). As a positive control, 2 wt% oil emulsions were prepared directly with PPO extract according to the original protocol. Further, emulsions of 2, 4, 6, 8 and 10 wt% oil were prepared, with 80 wt% PPO extract (experiment 2), or with 90, 80, 70, 60 and 50 wt% PPO extract, respectively (experiment 3) starting from WPI-stabilized emulsions. Enzymatic crosslinking was induced by 24-h incubation with 4MC. Ruminal protection efficiency was evaluated by 24-h in vitro batch simulation of the rumen metabolism. In experiment 1, protection efficiencies were equal or higher than the control (85.5% to 92.5% v. 81.3%). In both experiments 2 and 3, high protection efficiencies (>80%) were achieved, except for emulsions containing 10 wt% oil emulsions (<50% protection), which showed oiling-off after enzymatic crosslinking. This study demonstrated that alternative emulsifier proteins can be used in combination with PPO extract to protect emulsified PUFA-rich oils against ruminal biohydrogenation. By applying the new protocol, 6.5 times less PPO extract was required. |
| first_indexed | 2024-12-20T03:33:47Z |
| format | Article |
| id | doaj.art-70c61dfd8ee84f52b3535e45c7bff67b |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-20T03:33:47Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-70c61dfd8ee84f52b3535e45c7bff67b2022-12-21T19:54:55ZengElsevierAnimal1751-73112018-01-01121225392550Promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol-oxidase-mediated crosslinking of interfacial protein in emulsionsN. De Neve0B. Vlaeminck1F. Gadeyne2E. Claeys3P. Van der Meeren4V. Fievez5Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Coupure Links 653, Block F, 9000 Ghent, BelgiumLaboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Coupure Links 653, Block F, 9000 Ghent, BelgiumLaboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Coupure Links 653, Block F, 9000 Ghent, BelgiumLaboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Coupure Links 653, Block F, 9000 Ghent, BelgiumParticle and Interfacial Technology Group, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Block B, 9000 Ghent, BelgiumLaboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Coupure Links 653, Block F, 9000 Ghent, BelgiumPreviously, polyunsaturated fatty acids (PUFA) from linseed oil were effectively protected (>80%) against biohydrogenation through polyphenol-oxidase-mediated protein crosslinking of an emulsion, prepared with polyphenol oxidase (PPO) extract from potato tuber peelings. However, until now, emulsions of only 2 wt% oil have been successfully protected, which implies serious limitations both from a research perspective (e.g. in vivo trials) as well as for further upscaling toward practical applications. Therefore, the aim of this study was to increase the oil/PPO ratio. In the original protocol, the PPO extract served both an emulsifying function as well as a crosslinking function. Here, it was first evaluated whether alternative protein sources could replace the emulsifying function of the PPO extract, with addition of PPO extract and 4-methylcatechol (4MC) to induce crosslinking after emulsion preparation. This approach was then further used to evaluate protection of emulsions with higher oil content. Five candidate emulsifiers (soy glycinin, gelatin, whey protein isolate (WPI), bovine serum albumin and sodium caseinate) were used to prepare 10 wt% oil emulsions, which were diluted five times (w/w) with PPO extract (experiment 1). As a positive control, 2 wt% oil emulsions were prepared directly with PPO extract according to the original protocol. Further, emulsions of 2, 4, 6, 8 and 10 wt% oil were prepared, with 80 wt% PPO extract (experiment 2), or with 90, 80, 70, 60 and 50 wt% PPO extract, respectively (experiment 3) starting from WPI-stabilized emulsions. Enzymatic crosslinking was induced by 24-h incubation with 4MC. Ruminal protection efficiency was evaluated by 24-h in vitro batch simulation of the rumen metabolism. In experiment 1, protection efficiencies were equal or higher than the control (85.5% to 92.5% v. 81.3%). In both experiments 2 and 3, high protection efficiencies (>80%) were achieved, except for emulsions containing 10 wt% oil emulsions (<50% protection), which showed oiling-off after enzymatic crosslinking. This study demonstrated that alternative emulsifier proteins can be used in combination with PPO extract to protect emulsified PUFA-rich oils against ruminal biohydrogenation. By applying the new protocol, 6.5 times less PPO extract was required.http://www.sciencedirect.com/science/article/pii/S1751731118000423rumen-bypasssodium dodecyl sulfate polyacrylamide gel electrophoresiszymographyin vitrotyrosinase |
| spellingShingle | N. De Neve B. Vlaeminck F. Gadeyne E. Claeys P. Van der Meeren V. Fievez Promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol-oxidase-mediated crosslinking of interfacial protein in emulsions Animal rumen-bypass sodium dodecyl sulfate polyacrylamide gel electrophoresis zymography in vitro tyrosinase |
| title | Promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol-oxidase-mediated crosslinking of interfacial protein in emulsions |
| title_full | Promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol-oxidase-mediated crosslinking of interfacial protein in emulsions |
| title_fullStr | Promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol-oxidase-mediated crosslinking of interfacial protein in emulsions |
| title_full_unstemmed | Promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol-oxidase-mediated crosslinking of interfacial protein in emulsions |
| title_short | Promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol-oxidase-mediated crosslinking of interfacial protein in emulsions |
| title_sort | promising perspectives for ruminal protection of polyunsaturated fatty acids through polyphenol oxidase mediated crosslinking of interfacial protein in emulsions |
| topic | rumen-bypass sodium dodecyl sulfate polyacrylamide gel electrophoresis zymography in vitro tyrosinase |
| url | http://www.sciencedirect.com/science/article/pii/S1751731118000423 |
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