Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15
Chemosensory genes play important roles in insect behaviors and have thus become potential molecular targets for pest control based on the manipulation of chemoreception-driven behaviors. The great gray weevil Sympiezomias velatus (Chevrolat) (Coleoptera: Curculionidae) is an important agricultural...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.907667/full |
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| author | Xiao Li Jian-Wen Li Jian-Wen Li Wen-Xiu Sun Wei Li Hua-Yuan Gao Tong-Xian Liu Ming-Jing Qu |
| author_facet | Xiao Li Jian-Wen Li Jian-Wen Li Wen-Xiu Sun Wei Li Hua-Yuan Gao Tong-Xian Liu Ming-Jing Qu |
| author_sort | Xiao Li |
| collection | DOAJ |
| description | Chemosensory genes play important roles in insect behaviors and have thus become potential molecular targets for pest control based on the manipulation of chemoreception-driven behaviors. The great gray weevil Sympiezomias velatus (Chevrolat) (Coleoptera: Curculionidae) is an important agricultural pest that causes serious economic losses to many crops in China, but its chemosensory genes have not been reported. Here we assembled the antennal transcriptomes of female and male adult S. velatus and revealed the major chemosensory genes necessary for olfaction. A total of 138 candidate chemosensory genes in six families were identified, including 41 encoding odorant-binding proteins (OBPs), 11 encoding chemosensory proteins (CSPs), 62 encoding odorant receptors (ORs), 15 encoding gustatory receptors (GRs), six encoding ionotropic receptors (IRs), and three encoding sensory neuron membrane proteins (SNMPs). We analyzed their phylogenetic relationship based on the amino acid sequences of these chemosensory-related protein families in S. velatus and other insects, and the expression profiles based on their antennal transcriptomes. Chemosensory genes that show antenna-abundant/specific or sex-biased expression were observed, suggesting that these genes might have functions in olfaction. Furthermore, we chose an antenna-abundant OBP belonging to ABPX subfamily, SvelOBP15, to investigate its binding property. The results showed that among 33 tested compounds, SvelOBP15 displayed high binding affinities (Ki = 7.36–12.94 μmol/L) with farnesol, nerolidol, limonene and diisobutyl phthalate, indicating that SvelOBP15 plays olfactory roles by binding and transporting specific plant volatiles. These findings will help us better understand the olfactory systems of S. velatus, and provide a basis for functional elucidation of these chemosensory genes. |
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| id | doaj.art-70c67f914a9e428ea93c61ed4f1af8ce |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-14T05:15:28Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-70c67f914a9e428ea93c61ed4f1af8ce2022-12-22T02:10:22ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-05-011310.3389/fphys.2022.907667907667Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15Xiao Li0Jian-Wen Li1Jian-Wen Li2Wen-Xiu Sun3Wei Li4Hua-Yuan Gao5Tong-Xian Liu6Ming-Jing Qu7Shandong Peanut Research Institute, Qingdao, ChinaCollege of Life Sciences, Yangtze University, Jingzhou, ChinaWeinan Product Quality Supervision and Inspection Institute, Weinan, ChinaCollege of Life Sciences, Yangtze University, Jingzhou, ChinaCollege of Life Sciences, Yangtze University, Jingzhou, ChinaPeanut Research Institute, Jilin Academy of Agricultural Sciences, Changchun, ChinaCollege of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, ChinaShandong Peanut Research Institute, Qingdao, ChinaChemosensory genes play important roles in insect behaviors and have thus become potential molecular targets for pest control based on the manipulation of chemoreception-driven behaviors. The great gray weevil Sympiezomias velatus (Chevrolat) (Coleoptera: Curculionidae) is an important agricultural pest that causes serious economic losses to many crops in China, but its chemosensory genes have not been reported. Here we assembled the antennal transcriptomes of female and male adult S. velatus and revealed the major chemosensory genes necessary for olfaction. A total of 138 candidate chemosensory genes in six families were identified, including 41 encoding odorant-binding proteins (OBPs), 11 encoding chemosensory proteins (CSPs), 62 encoding odorant receptors (ORs), 15 encoding gustatory receptors (GRs), six encoding ionotropic receptors (IRs), and three encoding sensory neuron membrane proteins (SNMPs). We analyzed their phylogenetic relationship based on the amino acid sequences of these chemosensory-related protein families in S. velatus and other insects, and the expression profiles based on their antennal transcriptomes. Chemosensory genes that show antenna-abundant/specific or sex-biased expression were observed, suggesting that these genes might have functions in olfaction. Furthermore, we chose an antenna-abundant OBP belonging to ABPX subfamily, SvelOBP15, to investigate its binding property. The results showed that among 33 tested compounds, SvelOBP15 displayed high binding affinities (Ki = 7.36–12.94 μmol/L) with farnesol, nerolidol, limonene and diisobutyl phthalate, indicating that SvelOBP15 plays olfactory roles by binding and transporting specific plant volatiles. These findings will help us better understand the olfactory systems of S. velatus, and provide a basis for functional elucidation of these chemosensory genes.https://www.frontiersin.org/articles/10.3389/fphys.2022.907667/fullSympiezomias velatuschemosensory genestranscriptome analysisexpression patternodorant-binding proteinfluorescence competition binding assay |
| spellingShingle | Xiao Li Jian-Wen Li Jian-Wen Li Wen-Xiu Sun Wei Li Hua-Yuan Gao Tong-Xian Liu Ming-Jing Qu Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15 Frontiers in Physiology Sympiezomias velatus chemosensory genes transcriptome analysis expression pattern odorant-binding protein fluorescence competition binding assay |
| title | Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15 |
| title_full | Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15 |
| title_fullStr | Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15 |
| title_full_unstemmed | Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15 |
| title_short | Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15 |
| title_sort | candidate chemosensory genes identified in the adult antennae of sympiezomias velatus and binding property of odorant binding protein 15 |
| topic | Sympiezomias velatus chemosensory genes transcriptome analysis expression pattern odorant-binding protein fluorescence competition binding assay |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.907667/full |
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